Intermittent hypoxia inhibits mandibular cartilage growth with reduced TGF-β and SOX9 expressions in neonatal rats

Abstract Intermittent hypoxia (IH) has been associated with skeletal growth. However, the influence of IH on cartilage growth and metabolism is unknown. We compared the effects of IH on chondrocyte proliferation and maturation in the mandibular condyle fibrocartilage and tibial hyaline cartilage of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kochakorn Lekvijittada, Jun Hosomichi, Hideyuki Maeda, Haixin Hong, Chidsanu Changsiripun, Yo-ichiro Kuma, Shuji Oishi, Jun-ichi Suzuki, Ken-ichi Yoshida, Takashi Ono
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/910ee5c5a43240dfb76d0c871c2ff6a7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:910ee5c5a43240dfb76d0c871c2ff6a7
record_format	dspace
spelling	oai:doaj.org-article:910ee5c5a43240dfb76d0c871c2ff6a72021-12-02T14:01:23ZIntermittent hypoxia inhibits mandibular cartilage growth with reduced TGF-β and SOX9 expressions in neonatal rats10.1038/s41598-020-80303-32045-2322https://doaj.org/article/910ee5c5a43240dfb76d0c871c2ff6a72021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80303-3https://doaj.org/toc/2045-2322Abstract Intermittent hypoxia (IH) has been associated with skeletal growth. However, the influence of IH on cartilage growth and metabolism is unknown. We compared the effects of IH on chondrocyte proliferation and maturation in the mandibular condyle fibrocartilage and tibial hyaline cartilage of 1-week-old male Sprague–Dawley rats. The rats were exposed to normoxic air (n = 9) or IH at 20 cycles/h (nadir, 4% O2; peak, 21% O2; 0% CO2) (n = 9) for 8 h each day. IH impeded body weight gain, but not tibial elongation. IH also increased cancellous bone mineral and volumetric bone mineral densities in the mandibular condylar head. The mandibular condylar became thinner, but the tibial cartilage did not. IH reduced maturative and increased hypertrophic chondrocytic layers of the middle and posterior mandibular cartilage. PCR showed that IH shifted proliferation and maturation in mandibular condyle fibrocartilage toward hypertrophic differentiation and ossification by downregulating TGF-β and SOX9, and upregulating collagen X. These effects were absent in the tibial growth plate hyaline cartilage. Our results showed that neonatal rats exposed to IH displayed underdeveloped mandibular ramus/condyles, while suppression of chondrogenesis marker expression was detected in the growth-restricted condylar cartilage.Kochakorn LekvijittadaJun HosomichiHideyuki MaedaHaixin HongChidsanu ChangsiripunYo-ichiro KumaShuji OishiJun-ichi SuzukiKen-ichi YoshidaTakashi OnoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kochakorn Lekvijittada Jun Hosomichi Hideyuki Maeda Haixin Hong Chidsanu Changsiripun Yo-ichiro Kuma Shuji Oishi Jun-ichi Suzuki Ken-ichi Yoshida Takashi Ono Intermittent hypoxia inhibits mandibular cartilage growth with reduced TGF-β and SOX9 expressions in neonatal rats
description	Abstract Intermittent hypoxia (IH) has been associated with skeletal growth. However, the influence of IH on cartilage growth and metabolism is unknown. We compared the effects of IH on chondrocyte proliferation and maturation in the mandibular condyle fibrocartilage and tibial hyaline cartilage of 1-week-old male Sprague–Dawley rats. The rats were exposed to normoxic air (n = 9) or IH at 20 cycles/h (nadir, 4% O2; peak, 21% O2; 0% CO2) (n = 9) for 8 h each day. IH impeded body weight gain, but not tibial elongation. IH also increased cancellous bone mineral and volumetric bone mineral densities in the mandibular condylar head. The mandibular condylar became thinner, but the tibial cartilage did not. IH reduced maturative and increased hypertrophic chondrocytic layers of the middle and posterior mandibular cartilage. PCR showed that IH shifted proliferation and maturation in mandibular condyle fibrocartilage toward hypertrophic differentiation and ossification by downregulating TGF-β and SOX9, and upregulating collagen X. These effects were absent in the tibial growth plate hyaline cartilage. Our results showed that neonatal rats exposed to IH displayed underdeveloped mandibular ramus/condyles, while suppression of chondrogenesis marker expression was detected in the growth-restricted condylar cartilage.
format	article
author	Kochakorn Lekvijittada Jun Hosomichi Hideyuki Maeda Haixin Hong Chidsanu Changsiripun Yo-ichiro Kuma Shuji Oishi Jun-ichi Suzuki Ken-ichi Yoshida Takashi Ono
author_facet	Kochakorn Lekvijittada Jun Hosomichi Hideyuki Maeda Haixin Hong Chidsanu Changsiripun Yo-ichiro Kuma Shuji Oishi Jun-ichi Suzuki Ken-ichi Yoshida Takashi Ono
author_sort	Kochakorn Lekvijittada
title	Intermittent hypoxia inhibits mandibular cartilage growth with reduced TGF-β and SOX9 expressions in neonatal rats
title_short	Intermittent hypoxia inhibits mandibular cartilage growth with reduced TGF-β and SOX9 expressions in neonatal rats
title_full	Intermittent hypoxia inhibits mandibular cartilage growth with reduced TGF-β and SOX9 expressions in neonatal rats
title_fullStr	Intermittent hypoxia inhibits mandibular cartilage growth with reduced TGF-β and SOX9 expressions in neonatal rats
title_full_unstemmed	Intermittent hypoxia inhibits mandibular cartilage growth with reduced TGF-β and SOX9 expressions in neonatal rats
title_sort	intermittent hypoxia inhibits mandibular cartilage growth with reduced tgf-β and sox9 expressions in neonatal rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/910ee5c5a43240dfb76d0c871c2ff6a7
work_keys_str_mv	AT kochakornlekvijittada intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats AT junhosomichi intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats AT hideyukimaeda intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats AT haixinhong intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats AT chidsanuchangsiripun intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats AT yoichirokuma intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats AT shujioishi intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats AT junichisuzuki intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats AT kenichiyoshida intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats AT takashiono intermittenthypoxiainhibitsmandibularcartilagegrowthwithreducedtgfbandsox9expressionsinneonatalrats
_version_	1718392156149776384

Intermittent hypoxia inhibits mandibular cartilage growth with reduced TGF-β and SOX9 expressions in neonatal rats

Ejemplares similares