Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates

Abstract Cell development and behavior are driven by internal genetic programming, but the external microenvironment is increasingly recognized as a significant factor in cell differentiation, migration, and in the case of cancer, metastatic progression. Yet it remains unclear how the microenvironme...

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Autores principales:	Szu-Yuan Chou, Chang-You Lin, Theresa Cassino, Li Wan, Philip R. LeDuc
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9115f51dcda446679347c17cd1169ff2
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spelling	oai:doaj.org-article:9115f51dcda446679347c17cd1169ff22021-12-02T15:10:05ZProbing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates10.1038/s41598-020-74575-y2045-2322https://doaj.org/article/9115f51dcda446679347c17cd1169ff22020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-74575-yhttps://doaj.org/toc/2045-2322Abstract Cell development and behavior are driven by internal genetic programming, but the external microenvironment is increasingly recognized as a significant factor in cell differentiation, migration, and in the case of cancer, metastatic progression. Yet it remains unclear how the microenvironment influences cell processes, especially when examining cell motility. One factor that affects cell motility is cell mechanics, which is known to be related to substrate stiffness. Examining how cells interact with each other in response to mechanically differential substrates would allow an increased understanding of their coordinated cell motility. In order to probe the effect of substrate stiffness on tumor related cells in greater detail, we created hard–soft–hard (HSH) polydimethylsiloxane (PDMS) substrates with alternating regions of different stiffness (200 and 800 kPa). We then cultured WI-38 fibroblasts and A549 epithelial cells to probe their motile response to the substrates. We found that when the 2 cell types were exposed simultaneously to the same substrate, fibroblasts moved at an increased speed over epithelial cells. Furthermore, the HSH substrate allowed us to physically guide and separate the different cell types based on their relative motile speed. We believe that this method and results will be important in a diversity of areas including mechanical microenvironment, cell motility, and cancer biology.Szu-Yuan ChouChang-You LinTheresa CassinoLi WanPhilip R. LeDucNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Szu-Yuan Chou Chang-You Lin Theresa Cassino Li Wan Philip R. LeDuc Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
description	Abstract Cell development and behavior are driven by internal genetic programming, but the external microenvironment is increasingly recognized as a significant factor in cell differentiation, migration, and in the case of cancer, metastatic progression. Yet it remains unclear how the microenvironment influences cell processes, especially when examining cell motility. One factor that affects cell motility is cell mechanics, which is known to be related to substrate stiffness. Examining how cells interact with each other in response to mechanically differential substrates would allow an increased understanding of their coordinated cell motility. In order to probe the effect of substrate stiffness on tumor related cells in greater detail, we created hard–soft–hard (HSH) polydimethylsiloxane (PDMS) substrates with alternating regions of different stiffness (200 and 800 kPa). We then cultured WI-38 fibroblasts and A549 epithelial cells to probe their motile response to the substrates. We found that when the 2 cell types were exposed simultaneously to the same substrate, fibroblasts moved at an increased speed over epithelial cells. Furthermore, the HSH substrate allowed us to physically guide and separate the different cell types based on their relative motile speed. We believe that this method and results will be important in a diversity of areas including mechanical microenvironment, cell motility, and cancer biology.
format	article
author	Szu-Yuan Chou Chang-You Lin Theresa Cassino Li Wan Philip R. LeDuc
author_facet	Szu-Yuan Chou Chang-You Lin Theresa Cassino Li Wan Philip R. LeDuc
author_sort	Szu-Yuan Chou
title	Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_short	Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_full	Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_fullStr	Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_full_unstemmed	Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_sort	probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9115f51dcda446679347c17cd1169ff2
work_keys_str_mv	AT szuyuanchou probingcoordinatedcoculturecancerrelatedmotilitythroughdifferentialmicrocompartmentalizedelasticsubstrates AT changyoulin probingcoordinatedcoculturecancerrelatedmotilitythroughdifferentialmicrocompartmentalizedelasticsubstrates AT theresacassino probingcoordinatedcoculturecancerrelatedmotilitythroughdifferentialmicrocompartmentalizedelasticsubstrates AT liwan probingcoordinatedcoculturecancerrelatedmotilitythroughdifferentialmicrocompartmentalizedelasticsubstrates AT philiprleduc probingcoordinatedcoculturecancerrelatedmotilitythroughdifferentialmicrocompartmentalizedelasticsubstrates
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Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates

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