Automated parasitological diagnosis in clinical microbiology laboratories

Abstract Although there is a low prevalence of parasitological infections in Europe, the diagnosis of intestinal parasites is still difficult and laborious for microbiology laboratories. Currently, antigen detection assays and molecular biology allow a more accurate diagnosis, but these techniques h...

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Autores principales:	Gema Fernández-Rivas, Belén Rivaya, Nona Romaní, Jun Hao Wang Wang, Mireya Alcaide, Lurdes Matas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/911f7ba98783447e8efc5e6023f0ee83
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spelling	oai:doaj.org-article:911f7ba98783447e8efc5e6023f0ee832021-12-02T16:05:54ZAutomated parasitological diagnosis in clinical microbiology laboratories10.1038/s41598-021-92441-32045-2322https://doaj.org/article/911f7ba98783447e8efc5e6023f0ee832021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92441-3https://doaj.org/toc/2045-2322Abstract Although there is a low prevalence of parasitological infections in Europe, the diagnosis of intestinal parasites is still difficult and laborious for microbiology laboratories. Currently, antigen detection assays and molecular biology allow a more accurate diagnosis, but these techniques have limitations as they cannot detect all the possible parasites present in the samples. The objective of the study was to evaluate the accuracy and the usefulness of automated microscopy SediMAX2 (77 Elektronika, Budapest, Hungary) in the detection of parasitic infections from feces. A total of 197 formol-fixed stool samples were processed in parallel by wet mount examination and by SediMAX2. Sensitivities, specificities and predictive values were analyzed, reaching a sensitivity of 89.51% and a specificity of 98.15% and a very good positive predictive value (99.22%). SediMAX2 is a good tool for a reliable diagnosis of intestinal parasitic infections. The rapid processing and the flexibilty of storage of images analyzed make its incorporation into the day to day laboratory routine recommendable.Gema Fernández-RivasBelén RivayaNona RomaníJun Hao Wang WangMireya AlcaideLurdes MatasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gema Fernández-Rivas Belén Rivaya Nona Romaní Jun Hao Wang Wang Mireya Alcaide Lurdes Matas Automated parasitological diagnosis in clinical microbiology laboratories
description	Abstract Although there is a low prevalence of parasitological infections in Europe, the diagnosis of intestinal parasites is still difficult and laborious for microbiology laboratories. Currently, antigen detection assays and molecular biology allow a more accurate diagnosis, but these techniques have limitations as they cannot detect all the possible parasites present in the samples. The objective of the study was to evaluate the accuracy and the usefulness of automated microscopy SediMAX2 (77 Elektronika, Budapest, Hungary) in the detection of parasitic infections from feces. A total of 197 formol-fixed stool samples were processed in parallel by wet mount examination and by SediMAX2. Sensitivities, specificities and predictive values were analyzed, reaching a sensitivity of 89.51% and a specificity of 98.15% and a very good positive predictive value (99.22%). SediMAX2 is a good tool for a reliable diagnosis of intestinal parasitic infections. The rapid processing and the flexibilty of storage of images analyzed make its incorporation into the day to day laboratory routine recommendable.
format	article
author	Gema Fernández-Rivas Belén Rivaya Nona Romaní Jun Hao Wang Wang Mireya Alcaide Lurdes Matas
author_facet	Gema Fernández-Rivas Belén Rivaya Nona Romaní Jun Hao Wang Wang Mireya Alcaide Lurdes Matas
author_sort	Gema Fernández-Rivas
title	Automated parasitological diagnosis in clinical microbiology laboratories
title_short	Automated parasitological diagnosis in clinical microbiology laboratories
title_full	Automated parasitological diagnosis in clinical microbiology laboratories
title_fullStr	Automated parasitological diagnosis in clinical microbiology laboratories
title_full_unstemmed	Automated parasitological diagnosis in clinical microbiology laboratories
title_sort	automated parasitological diagnosis in clinical microbiology laboratories
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/911f7ba98783447e8efc5e6023f0ee83
work_keys_str_mv	AT gemafernandezrivas automatedparasitologicaldiagnosisinclinicalmicrobiologylaboratories AT belenrivaya automatedparasitologicaldiagnosisinclinicalmicrobiologylaboratories AT nonaromani automatedparasitologicaldiagnosisinclinicalmicrobiologylaboratories AT junhaowangwang automatedparasitologicaldiagnosisinclinicalmicrobiologylaboratories AT mireyaalcaide automatedparasitologicaldiagnosisinclinicalmicrobiologylaboratories AT lurdesmatas automatedparasitologicaldiagnosisinclinicalmicrobiologylaboratories
_version_	1718385161562750976

Automated parasitological diagnosis in clinical microbiology laboratories

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