Exploring the Antimicrobial Action of Quaternary Amines against <italic toggle="yes">Acinetobacter baumannii</italic>

ABSTRACT Quaternary amine compounds (QAC) are potent antimicrobials used to prevent the spread of pathogenic bacteria. While they are known for their membrane-damaging properties, QAC action has been suggested to extend beyond the surface to intracellular targets. Here we characterize the range of a...

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Autores principales:	Gregory A. Knauf, Ashley L. Cunningham, Misha I. Kazi, Ian M. Riddington, Alexander A. Crofts, Vincent Cattoir, M. Stephen Trent, Bryan W. Davies
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Acinetobacter baumannii BZK antimicrobial benzalkonium chloride biocide clostridium Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/91278b41ccf8409788ff83a319fd4d25
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spelling	oai:doaj.org-article:91278b41ccf8409788ff83a319fd4d252021-11-15T15:53:26ZExploring the Antimicrobial Action of Quaternary Amines against <italic toggle="yes">Acinetobacter baumannii</italic>10.1128/mBio.02394-172150-7511https://doaj.org/article/91278b41ccf8409788ff83a319fd4d252018-03-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02394-17https://doaj.org/toc/2150-7511ABSTRACT Quaternary amine compounds (QAC) are potent antimicrobials used to prevent the spread of pathogenic bacteria. While they are known for their membrane-damaging properties, QAC action has been suggested to extend beyond the surface to intracellular targets. Here we characterize the range of action of the QAC biocide benzalkonium chloride (BZK) against the bacterial pathogen Acinetobacter baumannii. At high concentrations, BZK acts through membrane disruption, but at low concentrations we show that wide-spread protein aggregation is associated with BZK-induced cell death. Resistance to BZK is found to develop through ribosomal protein mutations that protect A. baumannii against BZK-induced protein aggregation. The multifunctional impact of BZK led us to discover that alternative QAC structures, with low human toxicity, retain potent action against multidrug-resistant A. baumannii, Staphylococcus aureus, and Clostridium difficile and present opportunities for their development as antibiotics. IMPORTANCE Quaternary amine compounds (QACs) are widely used to prevent the spread of bacterial pathogens, but our understanding of their mode of action is incomplete. Here we describe disruption of bacterial proteostasis as an unrecognized action of QAC antimicrobial action and uncover the potential of diverse QAC structures to act as multitarget antibiotics.Gregory A. KnaufAshley L. CunninghamMisha I. KaziIan M. RiddingtonAlexander A. CroftsVincent CattoirM. Stephen TrentBryan W. DaviesAmerican Society for MicrobiologyarticleAcinetobacter baumanniiBZKantimicrobialbenzalkonium chloridebiocideclostridiumMicrobiologyQR1-502ENmBio, Vol 9, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acinetobacter baumannii BZK antimicrobial benzalkonium chloride biocide clostridium Microbiology QR1-502
spellingShingle	Acinetobacter baumannii BZK antimicrobial benzalkonium chloride biocide clostridium Microbiology QR1-502 Gregory A. Knauf Ashley L. Cunningham Misha I. Kazi Ian M. Riddington Alexander A. Crofts Vincent Cattoir M. Stephen Trent Bryan W. Davies Exploring the Antimicrobial Action of Quaternary Amines against <italic toggle="yes">Acinetobacter baumannii</italic>
description	ABSTRACT Quaternary amine compounds (QAC) are potent antimicrobials used to prevent the spread of pathogenic bacteria. While they are known for their membrane-damaging properties, QAC action has been suggested to extend beyond the surface to intracellular targets. Here we characterize the range of action of the QAC biocide benzalkonium chloride (BZK) against the bacterial pathogen Acinetobacter baumannii. At high concentrations, BZK acts through membrane disruption, but at low concentrations we show that wide-spread protein aggregation is associated with BZK-induced cell death. Resistance to BZK is found to develop through ribosomal protein mutations that protect A. baumannii against BZK-induced protein aggregation. The multifunctional impact of BZK led us to discover that alternative QAC structures, with low human toxicity, retain potent action against multidrug-resistant A. baumannii, Staphylococcus aureus, and Clostridium difficile and present opportunities for their development as antibiotics. IMPORTANCE Quaternary amine compounds (QACs) are widely used to prevent the spread of bacterial pathogens, but our understanding of their mode of action is incomplete. Here we describe disruption of bacterial proteostasis as an unrecognized action of QAC antimicrobial action and uncover the potential of diverse QAC structures to act as multitarget antibiotics.
format	article
author	Gregory A. Knauf Ashley L. Cunningham Misha I. Kazi Ian M. Riddington Alexander A. Crofts Vincent Cattoir M. Stephen Trent Bryan W. Davies
author_facet	Gregory A. Knauf Ashley L. Cunningham Misha I. Kazi Ian M. Riddington Alexander A. Crofts Vincent Cattoir M. Stephen Trent Bryan W. Davies
author_sort	Gregory A. Knauf
title	Exploring the Antimicrobial Action of Quaternary Amines against <italic toggle="yes">Acinetobacter baumannii</italic>
title_short	Exploring the Antimicrobial Action of Quaternary Amines against <italic toggle="yes">Acinetobacter baumannii</italic>
title_full	Exploring the Antimicrobial Action of Quaternary Amines against <italic toggle="yes">Acinetobacter baumannii</italic>
title_fullStr	Exploring the Antimicrobial Action of Quaternary Amines against <italic toggle="yes">Acinetobacter baumannii</italic>
title_full_unstemmed	Exploring the Antimicrobial Action of Quaternary Amines against <italic toggle="yes">Acinetobacter baumannii</italic>
title_sort	exploring the antimicrobial action of quaternary amines against <italic toggle="yes">acinetobacter baumannii</italic>
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/91278b41ccf8409788ff83a319fd4d25
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Exploring the Antimicrobial Action of Quaternary Amines against <italic toggle="yes">Acinetobacter baumannii</italic>

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