Identification of Cellular Factors Required for SARS-CoV-2 Replication

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the recently emerged virus responsible for the COVID-19 pandemic. Clinical presentation can range from asymptomatic disease and mild respiratory tract infection to severe disease with lung injury, multiorgan failure, and death. SARS-CoV...

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Autores principales:	Aleksandra Synowiec, Malwina Jedrysik, Wojciech Branicki, Adrianna Klajmon, Jing Lei, Katarzyna Owczarek, Chen Suo, Artur Szczepanski, Jingru Wang, Pengyan Zhang, Pawel P. Labaj, Krzysztof Pyrc
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SARS-CoV-2 coronavirus CRISPR-Cas9 cellular factors mechanisms of infection viral pathogenesis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9128ce59c54442e9a977222ee21f4382
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spelling	oai:doaj.org-article:9128ce59c54442e9a977222ee21f43822021-11-25T17:12:08ZIdentification of Cellular Factors Required for SARS-CoV-2 Replication10.3390/cells101131592073-4409https://doaj.org/article/9128ce59c54442e9a977222ee21f43822021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3159https://doaj.org/toc/2073-4409Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the recently emerged virus responsible for the COVID-19 pandemic. Clinical presentation can range from asymptomatic disease and mild respiratory tract infection to severe disease with lung injury, multiorgan failure, and death. SARS-CoV-2 is the third animal coronavirus to emerge in humans in the 21st century, and coronaviruses appear to possess a unique ability to cross borders between species and infect a wide range of organisms. This is somewhat surprising as, except for the requirement of host cell receptors, cell–pathogen interactions are usually species-specific. Insights into these host–virus interactions will provide a deeper understanding of the process of SARS-CoV-2 infection and provide a means for the design and development of antiviral agents. In this study, we describe a complex analysis of SARS-CoV-2 infection using a genome-wide CRISPR-Cas9 knock-out system in HeLa cells overexpressing entry receptor angiotensin-converting enzyme 2 (ACE2). This platform allows for the identification of factors required for viral replication. This study was designed to include a high number of replicates (48 replicates; 16 biological repeats with 3 technical replicates each) to prevent data instability, remove sources of bias, and allow multifactorial bioinformatic analyses in order to study the resulting interaction network. The results obtained provide an interesting insight into the replication mechanisms of SARS-CoV-2.Aleksandra SynowiecMalwina JedrysikWojciech BranickiAdrianna KlajmonJing LeiKatarzyna OwczarekChen SuoArtur SzczepanskiJingru WangPengyan ZhangPawel P. LabajKrzysztof PyrcMDPI AGarticleSARS-CoV-2coronavirusCRISPR-Cas9cellular factorsmechanisms of infectionviral pathogenesisBiology (General)QH301-705.5ENCells, Vol 10, Iss 3159, p 3159 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 coronavirus CRISPR-Cas9 cellular factors mechanisms of infection viral pathogenesis Biology (General) QH301-705.5
spellingShingle	SARS-CoV-2 coronavirus CRISPR-Cas9 cellular factors mechanisms of infection viral pathogenesis Biology (General) QH301-705.5 Aleksandra Synowiec Malwina Jedrysik Wojciech Branicki Adrianna Klajmon Jing Lei Katarzyna Owczarek Chen Suo Artur Szczepanski Jingru Wang Pengyan Zhang Pawel P. Labaj Krzysztof Pyrc Identification of Cellular Factors Required for SARS-CoV-2 Replication
description	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the recently emerged virus responsible for the COVID-19 pandemic. Clinical presentation can range from asymptomatic disease and mild respiratory tract infection to severe disease with lung injury, multiorgan failure, and death. SARS-CoV-2 is the third animal coronavirus to emerge in humans in the 21st century, and coronaviruses appear to possess a unique ability to cross borders between species and infect a wide range of organisms. This is somewhat surprising as, except for the requirement of host cell receptors, cell–pathogen interactions are usually species-specific. Insights into these host–virus interactions will provide a deeper understanding of the process of SARS-CoV-2 infection and provide a means for the design and development of antiviral agents. In this study, we describe a complex analysis of SARS-CoV-2 infection using a genome-wide CRISPR-Cas9 knock-out system in HeLa cells overexpressing entry receptor angiotensin-converting enzyme 2 (ACE2). This platform allows for the identification of factors required for viral replication. This study was designed to include a high number of replicates (48 replicates; 16 biological repeats with 3 technical replicates each) to prevent data instability, remove sources of bias, and allow multifactorial bioinformatic analyses in order to study the resulting interaction network. The results obtained provide an interesting insight into the replication mechanisms of SARS-CoV-2.
format	article
author	Aleksandra Synowiec Malwina Jedrysik Wojciech Branicki Adrianna Klajmon Jing Lei Katarzyna Owczarek Chen Suo Artur Szczepanski Jingru Wang Pengyan Zhang Pawel P. Labaj Krzysztof Pyrc
author_facet	Aleksandra Synowiec Malwina Jedrysik Wojciech Branicki Adrianna Klajmon Jing Lei Katarzyna Owczarek Chen Suo Artur Szczepanski Jingru Wang Pengyan Zhang Pawel P. Labaj Krzysztof Pyrc
author_sort	Aleksandra Synowiec
title	Identification of Cellular Factors Required for SARS-CoV-2 Replication
title_short	Identification of Cellular Factors Required for SARS-CoV-2 Replication
title_full	Identification of Cellular Factors Required for SARS-CoV-2 Replication
title_fullStr	Identification of Cellular Factors Required for SARS-CoV-2 Replication
title_full_unstemmed	Identification of Cellular Factors Required for SARS-CoV-2 Replication
title_sort	identification of cellular factors required for sars-cov-2 replication
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9128ce59c54442e9a977222ee21f4382
work_keys_str_mv	AT aleksandrasynowiec identificationofcellularfactorsrequiredforsarscov2replication AT malwinajedrysik identificationofcellularfactorsrequiredforsarscov2replication AT wojciechbranicki identificationofcellularfactorsrequiredforsarscov2replication AT adriannaklajmon identificationofcellularfactorsrequiredforsarscov2replication AT jinglei identificationofcellularfactorsrequiredforsarscov2replication AT katarzynaowczarek identificationofcellularfactorsrequiredforsarscov2replication AT chensuo identificationofcellularfactorsrequiredforsarscov2replication AT arturszczepanski identificationofcellularfactorsrequiredforsarscov2replication AT jingruwang identificationofcellularfactorsrequiredforsarscov2replication AT pengyanzhang identificationofcellularfactorsrequiredforsarscov2replication AT pawelplabaj identificationofcellularfactorsrequiredforsarscov2replication AT krzysztofpyrc identificationofcellularfactorsrequiredforsarscov2replication
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Identification of Cellular Factors Required for SARS-CoV-2 Replication

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