Lung and Gut Microbiota Changes Associated with <i>Pseudomonas aeruginosa</i> Infection in Mouse Models of Cystic Fibrosis

Cystic fibrosis (CF) disease leads to altered lung and gut microbiomes compared to healthy subjects. The magnitude of this dysbiosis is influenced by organ-specific microenvironmental conditions at different stages of the disease. However, how this gut-lung dysbiosis is influenced by <i>Pseudo...

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Autores principales:	Giovanni Bacci, Alice Rossi, Federica Armanini, Lisa Cangioli, Ida De Fino, Nicola Segata, Alessio Mengoni, Alessandra Bragonzi, Annamaria Bevivino
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cystic fibrosis lung gut microbiome gut-lung axis animal models Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/912967eee91345e895c0a57e24294f96
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spelling	oai:doaj.org-article:912967eee91345e895c0a57e24294f962021-11-25T17:53:57ZLung and Gut Microbiota Changes Associated with <i>Pseudomonas aeruginosa</i> Infection in Mouse Models of Cystic Fibrosis10.3390/ijms2222121691422-00671661-6596https://doaj.org/article/912967eee91345e895c0a57e24294f962021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12169https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cystic fibrosis (CF) disease leads to altered lung and gut microbiomes compared to healthy subjects. The magnitude of this dysbiosis is influenced by organ-specific microenvironmental conditions at different stages of the disease. However, how this gut-lung dysbiosis is influenced by <i>Pseudomonas aeruginosa</i> chronic infection is unclear. To test the relationship between CFTR dysfunction and gut-lung microbiome under chronic infection, we established a model of <i>P. aeruginosa</i> infection in wild-type (WT) and gut-corrected CF mice. Using 16S ribosomal RNA gene, we compared lung, stool, and gut microbiota of C57Bl/6 <i>Cftr <sup>tm1UNC</sup></i>TgN(FABP<i>CFTR</i>) or WT mice at the naïve state or infected with <i>P. aeruginosa.</i> <i>P. aeruginosa</i> infection influences murine health significantly changing body weight both in CF and WT mice. Both stool and gut microbiota revealed significantly higher values of alpha diversity in WT mice than in CF mice, while lung microbiota showed similar values. Infection with <i>P. aeruginosa</i> did not changed the diversity of the stool and gut microbiota, while a drop of diversity of the lung microbiota was observed compared to non-infected mice. However, the taxonomic composition of gut microbiota was shown to be influenced by <i>P. aeruginosa</i> infection in CF mice but not in WT mice. This finding indicates that <i>P. aeruginosa</i> chronic infection has a major impact on microbiota diversity and composition in the lung. In the gut, CFTR genotype and <i>P. aeruginosa</i> infection affected the overall diversity and taxonomic microbiota composition, respectively. Overall, our results suggest a cross-talk between lung and gut microbiota in relation to <i>P. aeruginosa</i> chronic infection and CFTR mutation.Giovanni BacciAlice RossiFederica ArmaniniLisa CangioliIda De FinoNicola SegataAlessio MengoniAlessandra BragonziAnnamaria BevivinoMDPI AGarticlecystic fibrosislunggutmicrobiomegut-lung axisanimal modelsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12169, p 12169 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cystic fibrosis lung gut microbiome gut-lung axis animal models Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	cystic fibrosis lung gut microbiome gut-lung axis animal models Biology (General) QH301-705.5 Chemistry QD1-999 Giovanni Bacci Alice Rossi Federica Armanini Lisa Cangioli Ida De Fino Nicola Segata Alessio Mengoni Alessandra Bragonzi Annamaria Bevivino Lung and Gut Microbiota Changes Associated with <i>Pseudomonas aeruginosa</i> Infection in Mouse Models of Cystic Fibrosis
description	Cystic fibrosis (CF) disease leads to altered lung and gut microbiomes compared to healthy subjects. The magnitude of this dysbiosis is influenced by organ-specific microenvironmental conditions at different stages of the disease. However, how this gut-lung dysbiosis is influenced by <i>Pseudomonas aeruginosa</i> chronic infection is unclear. To test the relationship between CFTR dysfunction and gut-lung microbiome under chronic infection, we established a model of <i>P. aeruginosa</i> infection in wild-type (WT) and gut-corrected CF mice. Using 16S ribosomal RNA gene, we compared lung, stool, and gut microbiota of C57Bl/6 <i>Cftr <sup>tm1UNC</sup></i>TgN(FABP<i>CFTR</i>) or WT mice at the naïve state or infected with <i>P. aeruginosa.</i> <i>P. aeruginosa</i> infection influences murine health significantly changing body weight both in CF and WT mice. Both stool and gut microbiota revealed significantly higher values of alpha diversity in WT mice than in CF mice, while lung microbiota showed similar values. Infection with <i>P. aeruginosa</i> did not changed the diversity of the stool and gut microbiota, while a drop of diversity of the lung microbiota was observed compared to non-infected mice. However, the taxonomic composition of gut microbiota was shown to be influenced by <i>P. aeruginosa</i> infection in CF mice but not in WT mice. This finding indicates that <i>P. aeruginosa</i> chronic infection has a major impact on microbiota diversity and composition in the lung. In the gut, CFTR genotype and <i>P. aeruginosa</i> infection affected the overall diversity and taxonomic microbiota composition, respectively. Overall, our results suggest a cross-talk between lung and gut microbiota in relation to <i>P. aeruginosa</i> chronic infection and CFTR mutation.
format	article
author	Giovanni Bacci Alice Rossi Federica Armanini Lisa Cangioli Ida De Fino Nicola Segata Alessio Mengoni Alessandra Bragonzi Annamaria Bevivino
author_facet	Giovanni Bacci Alice Rossi Federica Armanini Lisa Cangioli Ida De Fino Nicola Segata Alessio Mengoni Alessandra Bragonzi Annamaria Bevivino
author_sort	Giovanni Bacci
title	Lung and Gut Microbiota Changes Associated with <i>Pseudomonas aeruginosa</i> Infection in Mouse Models of Cystic Fibrosis
title_short	Lung and Gut Microbiota Changes Associated with <i>Pseudomonas aeruginosa</i> Infection in Mouse Models of Cystic Fibrosis
title_full	Lung and Gut Microbiota Changes Associated with <i>Pseudomonas aeruginosa</i> Infection in Mouse Models of Cystic Fibrosis
title_fullStr	Lung and Gut Microbiota Changes Associated with <i>Pseudomonas aeruginosa</i> Infection in Mouse Models of Cystic Fibrosis
title_full_unstemmed	Lung and Gut Microbiota Changes Associated with <i>Pseudomonas aeruginosa</i> Infection in Mouse Models of Cystic Fibrosis
title_sort	lung and gut microbiota changes associated with <i>pseudomonas aeruginosa</i> infection in mouse models of cystic fibrosis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/912967eee91345e895c0a57e24294f96
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Lung and Gut Microbiota Changes Associated with <i>Pseudomonas aeruginosa</i> Infection in Mouse Models of Cystic Fibrosis

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