Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection

Dengue virus infection mainly causes dengue hemorrhagic fever (DHF) and/or dengue shock syndrome (DSS). However, ADE (antibody-dependent enhancement) is one of the main pathogenic factors, and its pathogenic mechanism has not been fully elucidated. Recently, with the development of high-throughput s...

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Autores principales:	Mingwang Long, Yue Pan, Junying Chen, Fan Jia, Han Wang, Daiying Li, Kai Feng, Lingmei Yan, Xiaodan Wang, Xuelei Ning, Lijuan Qiu, Juan Zhang, Qiangming Sun
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	denv-3 (dengue virus serotype 3) ade (antibody-dependent enhancement) high-throughput sequencing rna-seq differentially expressed rnas (de rnas) rnas expression profile denv-host interactions immune system viral infectious diseases interferon-stimulated genes Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/9133276a38e04f5098c48ae38d814305
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spelling	oai:doaj.org-article:9133276a38e04f5098c48ae38d8143052021-11-11T14:23:42ZSweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection2150-55942150-560810.1080/21505594.2021.1996072https://doaj.org/article/9133276a38e04f5098c48ae38d8143052021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2021.1996072https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Dengue virus infection mainly causes dengue hemorrhagic fever (DHF) and/or dengue shock syndrome (DSS). However, ADE (antibody-dependent enhancement) is one of the main pathogenic factors, and its pathogenic mechanism has not been fully elucidated. Recently, with the development of high-throughput sequencing, an increased number of RNAs have been confirmed to play a vital regulatory role in the process of virus infection. However, there is a lack of research on dengue virus infection and ADE. In this study, we used RNA-Seq to detect differentially expressed RNAs (DE RNAs) profiles in mock-infected, DENV-3-infected, and ADE-infected THP-1 cells. Firstly, we found 69 circRNAs, 259 miRNAs, and 18 mRNAs were differentially expressed in THP-1 vs DENV-3. In THP-1 vs ADE, 94 circRNAs, 263 miRNAs, and 111 mRNAs were differentially expressed. In DENV-3 vs ADE, 68 circRNAs, 105 miRNAs, and 94 mRNAs were differentially expressed. Functional enrichment analysis of these DE RNAs mainly focused on immune system, viral infectious diseases, cytokine-cytokine receptor interactions, and NOD/RIG-I-like receptor signaling pathways. In DENV-3 vs ADE, notably, the expression of HBB was up-regulated, which was a Fcγ Receptor-mediated phagocytosis protein. Additionally, we predicted the encoding ability of DE circRNAs, and it was found that a small peptide was encoded by novel_circ_001562 and that its amino acid sequence was consistent with that of DDX60L, which is a class of interferon-stimulated genes. Finally, we constructed the ceRNA regulatory network pathway. Therefore, our study provides a new strategy for further investigation on DENV-host interactions.Mingwang LongYue PanJunying ChenFan JiaHan WangDaiying LiKai FengLingmei YanXiaodan WangXuelei NingLijuan QiuJuan ZhangQiangming SunTaylor & Francis Grouparticledenv-3 (dengue virus serotype 3)ade (antibody-dependent enhancement)high-throughput sequencingrna-seqdifferentially expressed rnas (de rnas)rnas expression profiledenv-host interactionsimmune systemviral infectious diseasesinterferon-stimulated genesInfectious and parasitic diseasesRC109-216ENVirulence, Vol 12, Iss 1, Pp 2764-2776 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	denv-3 (dengue virus serotype 3) ade (antibody-dependent enhancement) high-throughput sequencing rna-seq differentially expressed rnas (de rnas) rnas expression profile denv-host interactions immune system viral infectious diseases interferon-stimulated genes Infectious and parasitic diseases RC109-216
spellingShingle	denv-3 (dengue virus serotype 3) ade (antibody-dependent enhancement) high-throughput sequencing rna-seq differentially expressed rnas (de rnas) rnas expression profile denv-host interactions immune system viral infectious diseases interferon-stimulated genes Infectious and parasitic diseases RC109-216 Mingwang Long Yue Pan Junying Chen Fan Jia Han Wang Daiying Li Kai Feng Lingmei Yan Xiaodan Wang Xuelei Ning Lijuan Qiu Juan Zhang Qiangming Sun Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection
description	Dengue virus infection mainly causes dengue hemorrhagic fever (DHF) and/or dengue shock syndrome (DSS). However, ADE (antibody-dependent enhancement) is one of the main pathogenic factors, and its pathogenic mechanism has not been fully elucidated. Recently, with the development of high-throughput sequencing, an increased number of RNAs have been confirmed to play a vital regulatory role in the process of virus infection. However, there is a lack of research on dengue virus infection and ADE. In this study, we used RNA-Seq to detect differentially expressed RNAs (DE RNAs) profiles in mock-infected, DENV-3-infected, and ADE-infected THP-1 cells. Firstly, we found 69 circRNAs, 259 miRNAs, and 18 mRNAs were differentially expressed in THP-1 vs DENV-3. In THP-1 vs ADE, 94 circRNAs, 263 miRNAs, and 111 mRNAs were differentially expressed. In DENV-3 vs ADE, 68 circRNAs, 105 miRNAs, and 94 mRNAs were differentially expressed. Functional enrichment analysis of these DE RNAs mainly focused on immune system, viral infectious diseases, cytokine-cytokine receptor interactions, and NOD/RIG-I-like receptor signaling pathways. In DENV-3 vs ADE, notably, the expression of HBB was up-regulated, which was a Fcγ Receptor-mediated phagocytosis protein. Additionally, we predicted the encoding ability of DE circRNAs, and it was found that a small peptide was encoded by novel_circ_001562 and that its amino acid sequence was consistent with that of DDX60L, which is a class of interferon-stimulated genes. Finally, we constructed the ceRNA regulatory network pathway. Therefore, our study provides a new strategy for further investigation on DENV-host interactions.
format	article
author	Mingwang Long Yue Pan Junying Chen Fan Jia Han Wang Daiying Li Kai Feng Lingmei Yan Xiaodan Wang Xuelei Ning Lijuan Qiu Juan Zhang Qiangming Sun
author_facet	Mingwang Long Yue Pan Junying Chen Fan Jia Han Wang Daiying Li Kai Feng Lingmei Yan Xiaodan Wang Xuelei Ning Lijuan Qiu Juan Zhang Qiangming Sun
author_sort	Mingwang Long
title	Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection
title_short	Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection
title_full	Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection
title_fullStr	Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection
title_full_unstemmed	Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection
title_sort	sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/9133276a38e04f5098c48ae38d814305
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Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection

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