The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays

Introduction: Green tea and tocopherol are potent antioxidants used to treat melasma. However, whether green tea or tocopherol is superior remains unclear. Objective: To compare the effectiveness between green tea and tocopherol in decreasing the amount of melanin and tyrosinase level in wistar rats...

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Autores principales:	Anwar Riyanto, Taufiqurrachman Nasihun, Titiek Sumarawati
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2020
Materias:	green tea tocopherol amount of melanin tyrosinase level Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/91399995aef3413bbff824c63e64c22d
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spelling	oai:doaj.org-article:91399995aef3413bbff824c63e64c22d2021-11-25T13:16:36ZThe Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays2085-15452339-093X10.30659/sainsmed.v11i1.4347https://doaj.org/article/91399995aef3413bbff824c63e64c22d2020-07-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/4347https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XIntroduction: Green tea and tocopherol are potent antioxidants used to treat melasma. However, whether green tea or tocopherol is superior remains unclear. Objective: To compare the effectiveness between green tea and tocopherol in decreasing the amount of melanin and tyrosinase level in wistar rats exposed to UVB rays. Methods: The experimental study took 30 male Wistar rats randomly and divided them into 3 groups. The control group (C-G) was given basic topical cream, GT-G was given green tea topical cream and TC-G was given tocopherol topical cream. All rats were exposed to UVB every Monday, Wednesday and Friday for 4 weeks, whereas topical creams were smeared every day. Topical creams smearing on the same day with UVB exposure was performed 20 minutes before exposure and 4 hours after UVB exposure. The doses of UVB were 50 mJ/cm2 in first week, 70mJ/cm2 in the second week and 80mJ/cm2 in the third and fourth weeks. The amount of melanin was measured using pixel method and the tyrosinase level was measured using ELISA. Results: Anova analysis indicates that the amount of melanin and tyrosinase level are significantly different between groups, p<0.05. Post Hoc LSD analysis indicates that the amount of melanin in GT-G and TC-G are significantly lower than that of C-G, p<0.05. The amount of melanin in GT-G is lower than that of TC-G, p<0.05. The tyrosinase level in GT-G is significantly lower than that of C-G and TC-G, p<0.05. Meanwhile, the tyrosinase level in TC-G is lower than that of C-G but insignificantly, p>0.05. Conclusion: Green tea topical treatment is significantly capable of decreasing the amount of melanin and tyrosinase level better than tocopherol.Anwar RiyantoTaufiqurrachman NasihunTitiek SumarawatiUniversitas Sultan Agung Semarangarticlegreen teatocopherolamount of melanintyrosinase levelMedicine (General)R5-920ENSains Medika, Vol 11, Iss 1, Pp 20-26 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	green tea tocopherol amount of melanin tyrosinase level Medicine (General) R5-920
spellingShingle	green tea tocopherol amount of melanin tyrosinase level Medicine (General) R5-920 Anwar Riyanto Taufiqurrachman Nasihun Titiek Sumarawati The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays
description	Introduction: Green tea and tocopherol are potent antioxidants used to treat melasma. However, whether green tea or tocopherol is superior remains unclear. Objective: To compare the effectiveness between green tea and tocopherol in decreasing the amount of melanin and tyrosinase level in wistar rats exposed to UVB rays. Methods: The experimental study took 30 male Wistar rats randomly and divided them into 3 groups. The control group (C-G) was given basic topical cream, GT-G was given green tea topical cream and TC-G was given tocopherol topical cream. All rats were exposed to UVB every Monday, Wednesday and Friday for 4 weeks, whereas topical creams were smeared every day. Topical creams smearing on the same day with UVB exposure was performed 20 minutes before exposure and 4 hours after UVB exposure. The doses of UVB were 50 mJ/cm2 in first week, 70mJ/cm2 in the second week and 80mJ/cm2 in the third and fourth weeks. The amount of melanin was measured using pixel method and the tyrosinase level was measured using ELISA. Results: Anova analysis indicates that the amount of melanin and tyrosinase level are significantly different between groups, p<0.05. Post Hoc LSD analysis indicates that the amount of melanin in GT-G and TC-G are significantly lower than that of C-G, p<0.05. The amount of melanin in GT-G is lower than that of TC-G, p<0.05. The tyrosinase level in GT-G is significantly lower than that of C-G and TC-G, p<0.05. Meanwhile, the tyrosinase level in TC-G is lower than that of C-G but insignificantly, p>0.05. Conclusion: Green tea topical treatment is significantly capable of decreasing the amount of melanin and tyrosinase level better than tocopherol.
format	article
author	Anwar Riyanto Taufiqurrachman Nasihun Titiek Sumarawati
author_facet	Anwar Riyanto Taufiqurrachman Nasihun Titiek Sumarawati
author_sort	Anwar Riyanto
title	The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays
title_short	The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays
title_full	The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays
title_fullStr	The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays
title_full_unstemmed	The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays
title_sort	difference between the effect of green tea cream and tocopherol on decreasing level of tyrosinase enzyme and amount of melanin in rattus norvegicus exposed to uvb rays
publisher	Universitas Sultan Agung Semarang
publishDate	2020
url	https://doaj.org/article/91399995aef3413bbff824c63e64c22d
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The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays

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