Interaction between submicron COD crystals and renal epithelial cells

Hua Peng1,2 Jian-Ming Ouyang1,2 Xiu-Qiong Yao1, Ru-E Yang11Department of Chemistry, Jinan University, 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, ChinaObjectives: This study aims to investigate the adhesion characteristics between submicron calcium oxalate di...

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Autores principales:	Peng H, Ouyang JM, Yao XQ, Yang RE
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9141f22933a14dbfad46786be55485ad
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spelling	oai:doaj.org-article:9141f22933a14dbfad46786be55485ad2021-12-02T02:10:28ZInteraction between submicron COD crystals and renal epithelial cells1176-91141178-2013https://doaj.org/article/9141f22933a14dbfad46786be55485ad2012-08-01T00:00:00Zhttp://www.dovepress.com/interaction-between-submicron-cod-crystals-and-renal-epithelial-cells-a10861https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hua Peng1,2 Jian-Ming Ouyang1,2 Xiu-Qiong Yao1, Ru-E Yang11Department of Chemistry, Jinan University, 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, ChinaObjectives: This study aims to investigate the adhesion characteristics between submicron calcium oxalate dihydrate (COD) with a size of 150 &plusmn; 50 nm and African green monkey kidney epithelial cells (Vero cells) before and after damage, and to discuss the mechanism of kidney stone formation.Methods: Vero cells were oxidatively injured by hydrogen peroxide to establish a model of injured cells. Scanning electron microscopy was used to observe Vero&ndash;COD adhesion. Inductively coupled plasma emission spectrometry was used to quantitatively measure the amount of adhered COD microcrystals. Nanoparticle size analyzer and laser scanning confocal microscopy were performed to measure the change in the zeta potential on the Vero cell surface and the change in osteopontin expression during the adhesion process, respectively. The level of cell injury was evaluated by measuring the changes in malonaldehyde content, and cell viability during the adhesion process.Results: The adhesion capacity of Vero cells in the injury group to COD microcrystals was obviously stronger than that of Vero cells in the control group. After adhesion to COD, cell viability dropped, both malonaldehyde content and cell surface zeta potential increased, and the fluorescence intensity of osteopontin decreased because the osteopontin molecules were successfully covered by COD. Submicron COD further damaged the cells during the adhesion process, especially for Vero cells in the control group, leading to an elevated amount of attached microcrystals.Conclusion: Submicron COD can further damage injured Vero cells during the adhesion process. The amount of attached microcrystals is proportional to the degree of cell damage. The increased amount of microcrystals that adhered to the injured epithelial cells plays an important role in the formation of early-stage kidney stones.Keywords: cell modulation, crystal adhesion, calcium oxalate dihydrate, kidney stone, pathological mineralizationPeng HOuyang JMYao XQYang REDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4727-4737 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Peng H Ouyang JM Yao XQ Yang RE Interaction between submicron COD crystals and renal epithelial cells
description	Hua Peng1,2 Jian-Ming Ouyang1,2 Xiu-Qiong Yao1, Ru-E Yang11Department of Chemistry, Jinan University, 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, ChinaObjectives: This study aims to investigate the adhesion characteristics between submicron calcium oxalate dihydrate (COD) with a size of 150 &plusmn; 50 nm and African green monkey kidney epithelial cells (Vero cells) before and after damage, and to discuss the mechanism of kidney stone formation.Methods: Vero cells were oxidatively injured by hydrogen peroxide to establish a model of injured cells. Scanning electron microscopy was used to observe Vero&ndash;COD adhesion. Inductively coupled plasma emission spectrometry was used to quantitatively measure the amount of adhered COD microcrystals. Nanoparticle size analyzer and laser scanning confocal microscopy were performed to measure the change in the zeta potential on the Vero cell surface and the change in osteopontin expression during the adhesion process, respectively. The level of cell injury was evaluated by measuring the changes in malonaldehyde content, and cell viability during the adhesion process.Results: The adhesion capacity of Vero cells in the injury group to COD microcrystals was obviously stronger than that of Vero cells in the control group. After adhesion to COD, cell viability dropped, both malonaldehyde content and cell surface zeta potential increased, and the fluorescence intensity of osteopontin decreased because the osteopontin molecules were successfully covered by COD. Submicron COD further damaged the cells during the adhesion process, especially for Vero cells in the control group, leading to an elevated amount of attached microcrystals.Conclusion: Submicron COD can further damage injured Vero cells during the adhesion process. The amount of attached microcrystals is proportional to the degree of cell damage. The increased amount of microcrystals that adhered to the injured epithelial cells plays an important role in the formation of early-stage kidney stones.Keywords: cell modulation, crystal adhesion, calcium oxalate dihydrate, kidney stone, pathological mineralization
format	article
author	Peng H Ouyang JM Yao XQ Yang RE
author_facet	Peng H Ouyang JM Yao XQ Yang RE
author_sort	Peng H
title	Interaction between submicron COD crystals and renal epithelial cells
title_short	Interaction between submicron COD crystals and renal epithelial cells
title_full	Interaction between submicron COD crystals and renal epithelial cells
title_fullStr	Interaction between submicron COD crystals and renal epithelial cells
title_full_unstemmed	Interaction between submicron COD crystals and renal epithelial cells
title_sort	interaction between submicron cod crystals and renal epithelial cells
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/9141f22933a14dbfad46786be55485ad
work_keys_str_mv	AT pengh interactionbetweensubmicroncodcrystalsandrenalepithelialcells AT ouyangjm interactionbetweensubmicroncodcrystalsandrenalepithelialcells AT yaoxq interactionbetweensubmicroncodcrystalsandrenalepithelialcells AT yangre interactionbetweensubmicroncodcrystalsandrenalepithelialcells
_version_	1718402661683822592

Interaction between submicron COD crystals and renal epithelial cells

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