A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.

Comparative genomics has enabled the identification of genes that potentially evolved de novo from non-coding sequences. Many such genes are expressed in male reproductive tissues, but their functions remain poorly understood. To address this, we conducted a functional genetic screen of over 40 puta...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Emily L Rivard, Andrew G Ludwig, Prajal H Patel, Anna Grandchamp, Sarah E Arnold, Alina Berger, Emilie M Scott, Brendan J Kelly, Grace C Mascha, Erich Bornberg-Bauer, Geoffrey D Findlay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/914a8daee7114d5e9ee0eb6c73a363ec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:914a8daee7114d5e9ee0eb6c73a363ec
record_format	dspace
spelling	oai:doaj.org-article:914a8daee7114d5e9ee0eb6c73a363ec2021-12-02T20:03:22ZA putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.1553-73901553-740410.1371/journal.pgen.1009787https://doaj.org/article/914a8daee7114d5e9ee0eb6c73a363ec2021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009787https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Comparative genomics has enabled the identification of genes that potentially evolved de novo from non-coding sequences. Many such genes are expressed in male reproductive tissues, but their functions remain poorly understood. To address this, we conducted a functional genetic screen of over 40 putative de novo genes with testis-enriched expression in Drosophila melanogaster and identified one gene, atlas, required for male fertility. Detailed genetic and cytological analyses showed that atlas is required for proper chromatin condensation during the final stages of spermatogenesis. Atlas protein is expressed in spermatid nuclei and facilitates the transition from histone- to protamine-based chromatin packaging. Complementary evolutionary analyses revealed the complex evolutionary history of atlas. The protein-coding portion of the gene likely arose at the base of the Drosophila genus on the X chromosome but was unlikely to be essential, as it was then lost in several independent lineages. Within the last ~15 million years, however, the gene moved to an autosome, where it fused with a conserved non-coding RNA and evolved a non-redundant role in male fertility. Altogether, this study provides insight into the integration of novel genes into biological processes, the links between genomic innovation and functional evolution, and the genetic control of a fundamental developmental process, gametogenesis.Emily L RivardAndrew G LudwigPrajal H PatelAnna GrandchampSarah E ArnoldAlina BergerEmilie M ScottBrendan J KellyGrace C MaschaErich Bornberg-BauerGeoffrey D FindlayPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009787 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Emily L Rivard Andrew G Ludwig Prajal H Patel Anna Grandchamp Sarah E Arnold Alina Berger Emilie M Scott Brendan J Kelly Grace C Mascha Erich Bornberg-Bauer Geoffrey D Findlay A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.
description	Comparative genomics has enabled the identification of genes that potentially evolved de novo from non-coding sequences. Many such genes are expressed in male reproductive tissues, but their functions remain poorly understood. To address this, we conducted a functional genetic screen of over 40 putative de novo genes with testis-enriched expression in Drosophila melanogaster and identified one gene, atlas, required for male fertility. Detailed genetic and cytological analyses showed that atlas is required for proper chromatin condensation during the final stages of spermatogenesis. Atlas protein is expressed in spermatid nuclei and facilitates the transition from histone- to protamine-based chromatin packaging. Complementary evolutionary analyses revealed the complex evolutionary history of atlas. The protein-coding portion of the gene likely arose at the base of the Drosophila genus on the X chromosome but was unlikely to be essential, as it was then lost in several independent lineages. Within the last ~15 million years, however, the gene moved to an autosome, where it fused with a conserved non-coding RNA and evolved a non-redundant role in male fertility. Altogether, this study provides insight into the integration of novel genes into biological processes, the links between genomic innovation and functional evolution, and the genetic control of a fundamental developmental process, gametogenesis.
format	article
author	Emily L Rivard Andrew G Ludwig Prajal H Patel Anna Grandchamp Sarah E Arnold Alina Berger Emilie M Scott Brendan J Kelly Grace C Mascha Erich Bornberg-Bauer Geoffrey D Findlay
author_facet	Emily L Rivard Andrew G Ludwig Prajal H Patel Anna Grandchamp Sarah E Arnold Alina Berger Emilie M Scott Brendan J Kelly Grace C Mascha Erich Bornberg-Bauer Geoffrey D Findlay
author_sort	Emily L Rivard
title	A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.
title_short	A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.
title_full	A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.
title_fullStr	A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.
title_full_unstemmed	A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.
title_sort	putative de novo evolved gene required for spermatid chromatin condensation in drosophila melanogaster.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/914a8daee7114d5e9ee0eb6c73a363ec
work_keys_str_mv	AT emilylrivard aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT andrewgludwig aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT prajalhpatel aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT annagrandchamp aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT sarahearnold aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT alinaberger aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT emiliemscott aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT brendanjkelly aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT gracecmascha aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT erichbornbergbauer aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT geoffreydfindlay aputativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT emilylrivard putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT andrewgludwig putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT prajalhpatel putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT annagrandchamp putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT sarahearnold putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT alinaberger putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT emiliemscott putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT brendanjkelly putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT gracecmascha putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT erichbornbergbauer putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster AT geoffreydfindlay putativedenovoevolvedgenerequiredforspermatidchromatincondensationindrosophilamelanogaster
_version_	1718375672457461760

A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.

Ejemplares similares