Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses

A selective and sensitive assay was developed for colchicine in rat specimens. Colchicine and its deuterated analog (as internal standard, IS) were extracted from rat specimens (minimal 0.1 mL plasma, whole blood, or urine) using liquid-liquid extraction with n-hexane:dichloromethane:isopropanol. Th...

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Autores principales:	Hamdah M. Al Nebaihi, Tyson S. Le, Neal M. Davies, Dion R. Brocks
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	colchicine pharmacokinetics liquid-liquid extraction low dose preclinical small volume Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/914c40e3156f448790646f96eededa7f
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spelling	oai:doaj.org-article:914c40e3156f448790646f96eededa7f2021-11-25T18:51:25ZLiquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses10.3390/pr91120072227-9717https://doaj.org/article/914c40e3156f448790646f96eededa7f2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/2007https://doaj.org/toc/2227-9717A selective and sensitive assay was developed for colchicine in rat specimens. Colchicine and its deuterated analog (as internal standard, IS) were extracted from rat specimens (minimal 0.1 mL plasma, whole blood, or urine) using liquid-liquid extraction with n-hexane:dichloromethane:isopropanol. The mobile phase (formic acid: ammonium acetate: methanol) was pumped with uniform flow through an octadecylsilane analytical column. Detection was carried out by electrospray positive ionization in the multiple-reaction monitoring mode. The assay (total run time <3 min) had excellent linearity over a wide (400–800-fold) concentration range. The mean absolute recovery was >96.8%. The intra- and inter-day coefficients of variation were ˂15%, with lower limits of quantitation of 0.5 ng/mL in 0.1 mL of rat plasma. The method also provided the same lower limits of quantitation in urine and whole blood with 0.1 mL volumes, and 0.1 ng/mL using 0.5 mL of rat plasma. The blood-to-plasma ratio was >1. Rats had measurable colchicine blood concentrations for at least 24 h after intravenous doses of 0.1 mg/kg. The method possessed suitable measures of sensitivity and selectivity for detecting colchicine in several specimen types in rats given low doses.Hamdah M. Al NebaihiTyson S. LeNeal M. DaviesDion R. BrocksMDPI AGarticlecolchicinepharmacokineticsliquid-liquid extractionlow dosepreclinicalsmall volumeChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 2007, p 2007 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	colchicine pharmacokinetics liquid-liquid extraction low dose preclinical small volume Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	colchicine pharmacokinetics liquid-liquid extraction low dose preclinical small volume Chemical technology TP1-1185 Chemistry QD1-999 Hamdah M. Al Nebaihi Tyson S. Le Neal M. Davies Dion R. Brocks Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses
description	A selective and sensitive assay was developed for colchicine in rat specimens. Colchicine and its deuterated analog (as internal standard, IS) were extracted from rat specimens (minimal 0.1 mL plasma, whole blood, or urine) using liquid-liquid extraction with n-hexane:dichloromethane:isopropanol. The mobile phase (formic acid: ammonium acetate: methanol) was pumped with uniform flow through an octadecylsilane analytical column. Detection was carried out by electrospray positive ionization in the multiple-reaction monitoring mode. The assay (total run time <3 min) had excellent linearity over a wide (400–800-fold) concentration range. The mean absolute recovery was >96.8%. The intra- and inter-day coefficients of variation were ˂15%, with lower limits of quantitation of 0.5 ng/mL in 0.1 mL of rat plasma. The method also provided the same lower limits of quantitation in urine and whole blood with 0.1 mL volumes, and 0.1 ng/mL using 0.5 mL of rat plasma. The blood-to-plasma ratio was >1. Rats had measurable colchicine blood concentrations for at least 24 h after intravenous doses of 0.1 mg/kg. The method possessed suitable measures of sensitivity and selectivity for detecting colchicine in several specimen types in rats given low doses.
format	article
author	Hamdah M. Al Nebaihi Tyson S. Le Neal M. Davies Dion R. Brocks
author_facet	Hamdah M. Al Nebaihi Tyson S. Le Neal M. Davies Dion R. Brocks
author_sort	Hamdah M. Al Nebaihi
title	Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses
title_short	Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses
title_full	Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses
title_fullStr	Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses
title_full_unstemmed	Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses
title_sort	liquid chromatography tandem mass spectrometric analytical method for study of colchicine in rats given low doses
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/914c40e3156f448790646f96eededa7f
work_keys_str_mv	AT hamdahmalnebaihi liquidchromatographytandemmassspectrometricanalyticalmethodforstudyofcolchicineinratsgivenlowdoses AT tysonsle liquidchromatographytandemmassspectrometricanalyticalmethodforstudyofcolchicineinratsgivenlowdoses AT nealmdavies liquidchromatographytandemmassspectrometricanalyticalmethodforstudyofcolchicineinratsgivenlowdoses AT dionrbrocks liquidchromatographytandemmassspectrometricanalyticalmethodforstudyofcolchicineinratsgivenlowdoses
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Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses

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