Himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities

Bishnu Prasad Neupane, Dinesh Chaudhary, Sanjita Paudel, Sangita Timsina, Bipin Chapagain, Nirmala Jamarkattel, Bishnu Raj TiwariSchool of Health and Allied Sciences, Faculty of Health Sciences, Pokhara University, Pokhara, Kaski, NepalBackground: Himalayan honey, a natural product of wild honey bee...

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Autores principales:	Neupane BP, Chaudhary D, Paudel S, Timsina S, Chapagain B, Jamarkattel N, Tiwari BR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	honey biological activities nanoparticles cliff bee Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/91511521e3fd440f8544b5382513a048
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spelling	oai:doaj.org-article:91511521e3fd440f8544b5382513a0482021-12-02T04:45:03ZHimalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities1178-2013https://doaj.org/article/91511521e3fd440f8544b5382513a0482019-05-01T00:00:00Zhttps://www.dovepress.com/himalayan-honey-loaded-iron-oxide-nanoparticles-synthesis-characteriza-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Bishnu Prasad Neupane, Dinesh Chaudhary, Sanjita Paudel, Sangita Timsina, Bipin Chapagain, Nirmala Jamarkattel, Bishnu Raj TiwariSchool of Health and Allied Sciences, Faculty of Health Sciences, Pokhara University, Pokhara, Kaski, NepalBackground: Himalayan honey, a natural product of wild honey bees found in the Himalayan mountains of Nepal, has been used in medicine for many years. The successful development of nanotechnology and beneficial effects of honey would bring a new opportunity to synthesize hybrid nanomaterials for biomedical applications. Thus, the purpose of this study was to load Himalayan honey onto iron oxide nanoparticles (IO-NPs) and study their antioxidant and antimicrobial activities.Methods: Himalayan honey loaded iron oxide nanoparticles (HHLIO-NPs) were synthesized and X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses were performed for characterization. UV-VIS spectra confirmed the loading of honey onto nanoparticles. The antioxidant activity of these nanoparticles was studied against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical system. We also tested antimicrobial activity of HHLIO-NPs using well diffusion method towards both Gram-positive and Gram-negative bacterial strains of Staphylococus aureus & Escherichia coli.Results: From XRD analysis, the average particle size was found to be 33–40 nm. The SEM images show needle shape porous structures of HHLIO-NPs compared to free IO-NPs indicating the surfactant-like behaviour of honey. In DPPH radical system, the scavenging activities of Himalayan honey (HH), free IO-NPs and HHLIO-NPs ranged 7.93-35.99%, 11.02-52.02% and 16.10-80.52% respectively, with corresponding IC50 values of 1.36 mg/mL, 1.09 mg/mL and 0.52 mg/mL. The antimicrobial property of all test samples showed a noteworthy inhibition on both bacterial strains. However, the HH and HHLIO-NPs exhibited strong antibacterial activity against E. coli.Conclusion: This work reveals that the biological activity of HH is enhanced significantly after loading into IO-NPs. Thus, the HHLIO-NPs would be a promising alternative for antioxidant and antimicrobial agents.Keywords: honey, biological activities, nanoparticles, cliff beeNeupane BPChaudhary DPaudel STimsina SChapagain BJamarkattel NTiwari BRDove Medical Pressarticlehoneybiological activitiesnanoparticlescliff beeMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3533-3541 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	honey biological activities nanoparticles cliff bee Medicine (General) R5-920
spellingShingle	honey biological activities nanoparticles cliff bee Medicine (General) R5-920 Neupane BP Chaudhary D Paudel S Timsina S Chapagain B Jamarkattel N Tiwari BR Himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities
description	Bishnu Prasad Neupane, Dinesh Chaudhary, Sanjita Paudel, Sangita Timsina, Bipin Chapagain, Nirmala Jamarkattel, Bishnu Raj TiwariSchool of Health and Allied Sciences, Faculty of Health Sciences, Pokhara University, Pokhara, Kaski, NepalBackground: Himalayan honey, a natural product of wild honey bees found in the Himalayan mountains of Nepal, has been used in medicine for many years. The successful development of nanotechnology and beneficial effects of honey would bring a new opportunity to synthesize hybrid nanomaterials for biomedical applications. Thus, the purpose of this study was to load Himalayan honey onto iron oxide nanoparticles (IO-NPs) and study their antioxidant and antimicrobial activities.Methods: Himalayan honey loaded iron oxide nanoparticles (HHLIO-NPs) were synthesized and X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses were performed for characterization. UV-VIS spectra confirmed the loading of honey onto nanoparticles. The antioxidant activity of these nanoparticles was studied against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical system. We also tested antimicrobial activity of HHLIO-NPs using well diffusion method towards both Gram-positive and Gram-negative bacterial strains of Staphylococus aureus & Escherichia coli.Results: From XRD analysis, the average particle size was found to be 33–40 nm. The SEM images show needle shape porous structures of HHLIO-NPs compared to free IO-NPs indicating the surfactant-like behaviour of honey. In DPPH radical system, the scavenging activities of Himalayan honey (HH), free IO-NPs and HHLIO-NPs ranged 7.93-35.99%, 11.02-52.02% and 16.10-80.52% respectively, with corresponding IC50 values of 1.36 mg/mL, 1.09 mg/mL and 0.52 mg/mL. The antimicrobial property of all test samples showed a noteworthy inhibition on both bacterial strains. However, the HH and HHLIO-NPs exhibited strong antibacterial activity against E. coli.Conclusion: This work reveals that the biological activity of HH is enhanced significantly after loading into IO-NPs. Thus, the HHLIO-NPs would be a promising alternative for antioxidant and antimicrobial agents.Keywords: honey, biological activities, nanoparticles, cliff bee
format	article
author	Neupane BP Chaudhary D Paudel S Timsina S Chapagain B Jamarkattel N Tiwari BR
author_facet	Neupane BP Chaudhary D Paudel S Timsina S Chapagain B Jamarkattel N Tiwari BR
author_sort	Neupane BP
title	Himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities
title_short	Himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities
title_full	Himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities
title_fullStr	Himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities
title_full_unstemmed	Himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities
title_sort	himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/91511521e3fd440f8544b5382513a048
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Himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities

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