Two-color nanoscopy of organelles for extended times with HIDE probes
High density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods.
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Nature Portfolio
2020
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oai:doaj.org-article:91511d84bdfc4dfb9363382cbb7798312021-12-02T16:35:13ZTwo-color nanoscopy of organelles for extended times with HIDE probes10.1038/s41467-020-17859-12041-1723https://doaj.org/article/91511d84bdfc4dfb9363382cbb7798312020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17859-1https://doaj.org/toc/2041-1723High density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods.Ling ChuJonathan TysonJuliana E. ShawFelix Rivera-MolinaAnthony J. KoleskeAlanna SchepartzDerek K. ToomreNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020) |
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DOAJ |
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Science Q |
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Science Q Ling Chu Jonathan Tyson Juliana E. Shaw Felix Rivera-Molina Anthony J. Koleske Alanna Schepartz Derek K. Toomre Two-color nanoscopy of organelles for extended times with HIDE probes |
description |
High density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods. |
format |
article |
author |
Ling Chu Jonathan Tyson Juliana E. Shaw Felix Rivera-Molina Anthony J. Koleske Alanna Schepartz Derek K. Toomre |
author_facet |
Ling Chu Jonathan Tyson Juliana E. Shaw Felix Rivera-Molina Anthony J. Koleske Alanna Schepartz Derek K. Toomre |
author_sort |
Ling Chu |
title |
Two-color nanoscopy of organelles for extended times with HIDE probes |
title_short |
Two-color nanoscopy of organelles for extended times with HIDE probes |
title_full |
Two-color nanoscopy of organelles for extended times with HIDE probes |
title_fullStr |
Two-color nanoscopy of organelles for extended times with HIDE probes |
title_full_unstemmed |
Two-color nanoscopy of organelles for extended times with HIDE probes |
title_sort |
two-color nanoscopy of organelles for extended times with hide probes |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doaj.org/article/91511d84bdfc4dfb9363382cbb779831 |
work_keys_str_mv |
AT lingchu twocolornanoscopyoforganellesforextendedtimeswithhideprobes AT jonathantyson twocolornanoscopyoforganellesforextendedtimeswithhideprobes AT julianaeshaw twocolornanoscopyoforganellesforextendedtimeswithhideprobes AT felixriveramolina twocolornanoscopyoforganellesforextendedtimeswithhideprobes AT anthonyjkoleske twocolornanoscopyoforganellesforextendedtimeswithhideprobes AT alannaschepartz twocolornanoscopyoforganellesforextendedtimeswithhideprobes AT derekktoomre twocolornanoscopyoforganellesforextendedtimeswithhideprobes |
_version_ |
1718383705864536064 |