Two-color nanoscopy of organelles for extended times with HIDE probes

High density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods.

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Autores principales: Ling Chu, Jonathan Tyson, Juliana E. Shaw, Felix Rivera-Molina, Anthony J. Koleske, Alanna Schepartz, Derek K. Toomre
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/91511d84bdfc4dfb9363382cbb779831
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spelling oai:doaj.org-article:91511d84bdfc4dfb9363382cbb7798312021-12-02T16:35:13ZTwo-color nanoscopy of organelles for extended times with HIDE probes10.1038/s41467-020-17859-12041-1723https://doaj.org/article/91511d84bdfc4dfb9363382cbb7798312020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17859-1https://doaj.org/toc/2041-1723High density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods.Ling ChuJonathan TysonJuliana E. ShawFelix Rivera-MolinaAnthony J. KoleskeAlanna SchepartzDerek K. ToomreNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Ling Chu
Jonathan Tyson
Juliana E. Shaw
Felix Rivera-Molina
Anthony J. Koleske
Alanna Schepartz
Derek K. Toomre
Two-color nanoscopy of organelles for extended times with HIDE probes
description High density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods.
format article
author Ling Chu
Jonathan Tyson
Juliana E. Shaw
Felix Rivera-Molina
Anthony J. Koleske
Alanna Schepartz
Derek K. Toomre
author_facet Ling Chu
Jonathan Tyson
Juliana E. Shaw
Felix Rivera-Molina
Anthony J. Koleske
Alanna Schepartz
Derek K. Toomre
author_sort Ling Chu
title Two-color nanoscopy of organelles for extended times with HIDE probes
title_short Two-color nanoscopy of organelles for extended times with HIDE probes
title_full Two-color nanoscopy of organelles for extended times with HIDE probes
title_fullStr Two-color nanoscopy of organelles for extended times with HIDE probes
title_full_unstemmed Two-color nanoscopy of organelles for extended times with HIDE probes
title_sort two-color nanoscopy of organelles for extended times with hide probes
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/91511d84bdfc4dfb9363382cbb779831
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AT jonathantyson twocolornanoscopyoforganellesforextendedtimeswithhideprobes
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AT felixriveramolina twocolornanoscopyoforganellesforextendedtimeswithhideprobes
AT anthonyjkoleske twocolornanoscopyoforganellesforextendedtimeswithhideprobes
AT alannaschepartz twocolornanoscopyoforganellesforextendedtimeswithhideprobes
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