Interchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer
Abstract Virtual microscopy (VM) holds promise to reduce subjectivity as well as intra- and inter-observer variability for the histopathological evaluation of prostate cancer. We evaluated (i) the repeatability (intra-observer agreement) and reproducibility (inter-observer agreement) of the 2014 Gle...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/915495535cdb496195aceb745bef4d3f |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:915495535cdb496195aceb745bef4d3f |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:915495535cdb496195aceb745bef4d3f2021-12-02T14:06:50ZInterchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer10.1038/s41598-021-82911-z2045-2322https://doaj.org/article/915495535cdb496195aceb745bef4d3f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82911-zhttps://doaj.org/toc/2045-2322Abstract Virtual microscopy (VM) holds promise to reduce subjectivity as well as intra- and inter-observer variability for the histopathological evaluation of prostate cancer. We evaluated (i) the repeatability (intra-observer agreement) and reproducibility (inter-observer agreement) of the 2014 Gleason grading system and other selected features using standard light microscopy (LM) and an internally developed VM system, and (ii) the interchangeability of LM and VM. Two uro-pathologists reviewed 413 cores from 60 Swedish men diagnosed with non-metastatic prostate cancer 1998–2014. Reviewer 1 performed two reviews using both LM and VM. Reviewer 2 performed one review using both methods. The intra- and inter-observer agreement within and between LM and VM were assessed using Cohen’s kappa and Bland and Altman’s limits of agreement. We found good repeatability and reproducibility for both LM and VM, as well as interchangeability between LM and VM, for primary and secondary Gleason pattern, Gleason Grade Groups, poorly formed glands, cribriform pattern and comedonecrosis but not for the percentage of Gleason pattern 4. Our findings confirm the non-inferiority of VM compared to LM. The repeatability and reproducibility of percentage of Gleason pattern 4 was poor regardless of method used warranting further investigation and improvement before it is used in clinical practice.Renata ZelicFrancesca GiunchiLuca LianasCecilia MasciaGianluigi ZanettiOve AndrénJonna FridfeldtJessica CarlssonSabina DavidssonLuca MolinaroPer Henrik VincentLorenzo RichiardiOlof AkreMichelangelo FiorentinoAndreas PetterssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Renata Zelic Francesca Giunchi Luca Lianas Cecilia Mascia Gianluigi Zanetti Ove Andrén Jonna Fridfeldt Jessica Carlsson Sabina Davidsson Luca Molinaro Per Henrik Vincent Lorenzo Richiardi Olof Akre Michelangelo Fiorentino Andreas Pettersson Interchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer |
| description |
Abstract Virtual microscopy (VM) holds promise to reduce subjectivity as well as intra- and inter-observer variability for the histopathological evaluation of prostate cancer. We evaluated (i) the repeatability (intra-observer agreement) and reproducibility (inter-observer agreement) of the 2014 Gleason grading system and other selected features using standard light microscopy (LM) and an internally developed VM system, and (ii) the interchangeability of LM and VM. Two uro-pathologists reviewed 413 cores from 60 Swedish men diagnosed with non-metastatic prostate cancer 1998–2014. Reviewer 1 performed two reviews using both LM and VM. Reviewer 2 performed one review using both methods. The intra- and inter-observer agreement within and between LM and VM were assessed using Cohen’s kappa and Bland and Altman’s limits of agreement. We found good repeatability and reproducibility for both LM and VM, as well as interchangeability between LM and VM, for primary and secondary Gleason pattern, Gleason Grade Groups, poorly formed glands, cribriform pattern and comedonecrosis but not for the percentage of Gleason pattern 4. Our findings confirm the non-inferiority of VM compared to LM. The repeatability and reproducibility of percentage of Gleason pattern 4 was poor regardless of method used warranting further investigation and improvement before it is used in clinical practice. |
| format |
article |
| author |
Renata Zelic Francesca Giunchi Luca Lianas Cecilia Mascia Gianluigi Zanetti Ove Andrén Jonna Fridfeldt Jessica Carlsson Sabina Davidsson Luca Molinaro Per Henrik Vincent Lorenzo Richiardi Olof Akre Michelangelo Fiorentino Andreas Pettersson |
| author_facet |
Renata Zelic Francesca Giunchi Luca Lianas Cecilia Mascia Gianluigi Zanetti Ove Andrén Jonna Fridfeldt Jessica Carlsson Sabina Davidsson Luca Molinaro Per Henrik Vincent Lorenzo Richiardi Olof Akre Michelangelo Fiorentino Andreas Pettersson |
| author_sort |
Renata Zelic |
| title |
Interchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer |
| title_short |
Interchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer |
| title_full |
Interchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer |
| title_fullStr |
Interchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer |
| title_full_unstemmed |
Interchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer |
| title_sort |
interchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/915495535cdb496195aceb745bef4d3f |
| work_keys_str_mv |
AT renatazelic interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT francescagiunchi interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT lucalianas interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT ceciliamascia interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT gianluigizanetti interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT oveandren interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT jonnafridfeldt interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT jessicacarlsson interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT sabinadavidsson interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT lucamolinaro interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT perhenrikvincent interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT lorenzorichiardi interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT olofakre interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT michelangelofiorentino interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer AT andreaspettersson interchangeabilityoflightandvirtualmicroscopyforhistopathologicalevaluationofprostatecancer |
| _version_ |
1718391977356034048 |