Species-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein

Species-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein

Joana S Maziero,1 Velaphi C Thipe,1,2 Sizue O Rogero,1 Adriana K Cavalcante,1 Kelme C Damasceno,1 Matheus B Ormenio,1 Gisela A Martini,1 Jorge GS Batista,3 William Viveiros,4 Kavita K Katti,2 Alice Raphael Karikachery,2 DarshaKumar Dhurvas Mohandoss,5 Rashmi DarshaKumar Dhurvas,5 Mohanavelu Nappinna...

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Autores principales: Maziero JS, Thipe VC, Rogero SO, Cavalcante AK, Damasceno KC, Ormenio MB, Martini GA, Batista JGS, Viveiros W, Katti KK, Raphael Karikachery A, Dhurvas Mohandoss D, Dhurvas RD, Nappinnai M, Rogero JR, Lugão AB, Katti KV
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2020
Materias:
nanoparticles
zebrafish
cytotoxicity
ecotoxicity
daphnia similis
sprague dawley.
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/9160eef401c54afb9e71e8566bddcbc5
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id oai:doaj.org-article:9160eef401c54afb9e71e8566bddcbc5
record_format dspace
institution DOAJ
collection DOAJ
language EN
topic nanoparticles
zebrafish
cytotoxicity
ecotoxicity
daphnia similis
sprague dawley.
Medicine (General)
R5-920
spellingShingle nanoparticles
zebrafish
cytotoxicity
ecotoxicity
daphnia similis
sprague dawley.
Medicine (General)
R5-920
Maziero JS
Thipe VC
Rogero SO
Cavalcante AK
Damasceno KC
Ormenio MB
Martini GA
Batista JGS
Viveiros W
Katti KK
Raphael Karikachery A
Dhurvas Mohandoss D
Dhurvas RD
Nappinnai M
Rogero JR
Lugão AB
Katti KV
Species-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein
description Joana S Maziero,1 Velaphi C Thipe,1,2 Sizue O Rogero,1 Adriana K Cavalcante,1 Kelme C Damasceno,1 Matheus B Ormenio,1 Gisela A Martini,1 Jorge GS Batista,3 William Viveiros,4 Kavita K Katti,2 Alice Raphael Karikachery,2 DarshaKumar Dhurvas Mohandoss,5 Rashmi DarshaKumar Dhurvas,5 Mohanavelu Nappinnai,5 José R Rogero,3 Ademar B Lugão,1 Kattesh V Katti2 1Laboratório de Ecotoxicologia, Centro de Química e Meio Ambiente, Instituto de Pesquisas Energéticas e Nucleares (IPEN), Comissão Nacional de Energia Nuclear- IPEN/CNEN-SP, São Paulo, SP, Brazil; 2Institute of Green Nanotechnology, Department of Radiology, University of Missouri Columbia, Columbia, MO, USA; 3Laboratório de Biomateriais Poliméricos e Nanotheranóstica, Centro de Química e Meio Ambiente, Instituto de Pesquisas Energéticas e Nucleares (IPEN), Comissão Nacional de Energia Nuclear- IPEN/CNEN-SP, São Paulo, SP, Brazil; 4Laboratório de Ecotoxicologia, Companhia Ambiental do Estado de São Paulo (CETESB), São Paulo, SP, Brazil; 5Dhanvantari Nano Ayushadi Pvt Ltd, Chennai, Tamil Nadu, IndiaCorrespondence: Kattesh V Katti; Ademar B Lugão Email kattik@health.missouri.edu; ablugao@gmail.comIntroduction: We report, herein, in vitro, and in vivo toxicity evaluation of silver nanoparticles stabilized with gum arabic protein (AgNP-GP) in Daphnia similis, Danio rerio embryos and in Sprague Dawley rats.Purpose: The objective of this investigation was to evaluate in vitro and in vivo toxicity of silver nanoparticles stabilized with gum arabic protein (AgNP-GP), in multispecies due to the recognition that toxicity evaluations beyond a single species reflect the environmental realism. In the present study, AgNP-GP was synthesized through the reduction of silver salt using the tri-alanine-phosphine peptide (commonly referred to as “Katti Peptide”) and stabilized using gum arabic protein.Methods: In vitro cytotoxicity tests were performed according to ISO 10993– 5 protocols to assess cytotoxicity index (IC50) values. Acute ecotoxicity (EC50) studies were performed using Daphnia similis, according to the ABNT NBR 15088 protocols. In vivo toxicity also included evaluation of acute embryotoxicity using Danio rerio (zebrafish) embryos following the OECD No. 236 guidelines. We also used Sprague Dawley rats to assess the toxicity of AgNP-GP in doses from 2.5 to 10.0 mg kg− 1 body weight.Results: AgNP-GP nanoparticles were characterized through UV (405 nm), core size (20± 5 nm through TEM), hydrodynamic size (70– 80 nm), Zeta (ζ) potential (- 26 mV) using DLS and Powder X ray diffraction (PXRD) and EDS. PXRD showed pattern consistent with the Ag (1 1 1) peak. EC50 in Daphnia similis was 4.40 (3.59– 5.40) μg L− 1. In the zebrafish species, LC50 was 177 μg L− 1. Oral administration of AgNP-GP in Sprague Dawley rats for a period of 28 days revealed no adverse effects in doses of up to 10.0 mg kg− 1 b.w. in both male and female animals.Conclusion: The non-toxicity of AgNP-GP in rats offers a myriad of applications of AgNP-GP in health and hygiene for use as antibiotics, antimicrobial and antifungal agents.Keywords: nanoparticles, zebrafish, cytotoxicity, ecotoxicity, Daphnia similis, Sprague Dawley
format article
author Maziero JS
Thipe VC
Rogero SO
Cavalcante AK
Damasceno KC
Ormenio MB
Martini GA
Batista JGS
Viveiros W
Katti KK
Raphael Karikachery A
Dhurvas Mohandoss D
Dhurvas RD
Nappinnai M
Rogero JR
Lugão AB
Katti KV
author_facet Maziero JS
Thipe VC
Rogero SO
Cavalcante AK
Damasceno KC
Ormenio MB
Martini GA
Batista JGS
Viveiros W
Katti KK
Raphael Karikachery A
Dhurvas Mohandoss D
Dhurvas RD
Nappinnai M
Rogero JR
Lugão AB
Katti KV
author_sort Maziero JS
title Species-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein
title_short Species-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein
title_full Species-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein
title_fullStr Species-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein
title_full_unstemmed Species-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein
title_sort species-specific in vitro and in vivo evaluation of toxicity of silver nanoparticles stabilized with gum arabic protein
publisher Dove Medical Press
publishDate 2020
url https://doaj.org/article/9160eef401c54afb9e71e8566bddcbc5
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spelling oai:doaj.org-article:9160eef401c54afb9e71e8566bddcbc52021-12-02T11:16:05ZSpecies-Specific in vitro and in vivo Evaluation of Toxicity of Silver Nanoparticles Stabilized with Gum Arabic Protein1178-2013https://doaj.org/article/9160eef401c54afb9e71e8566bddcbc52020-10-01T00:00:00Zhttps://www.dovepress.com/species-specific-in-vitro-and-in-vivo-evaluation-of-toxicity-of-silver-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Joana S Maziero,1 Velaphi C Thipe,1,2 Sizue O Rogero,1 Adriana K Cavalcante,1 Kelme C Damasceno,1 Matheus B Ormenio,1 Gisela A Martini,1 Jorge GS Batista,3 William Viveiros,4 Kavita K Katti,2 Alice Raphael Karikachery,2 DarshaKumar Dhurvas Mohandoss,5 Rashmi DarshaKumar Dhurvas,5 Mohanavelu Nappinnai,5 José R Rogero,3 Ademar B Lugão,1 Kattesh V Katti2 1Laboratório de Ecotoxicologia, Centro de Química e Meio Ambiente, Instituto de Pesquisas Energéticas e Nucleares (IPEN), Comissão Nacional de Energia Nuclear- IPEN/CNEN-SP, São Paulo, SP, Brazil; 2Institute of Green Nanotechnology, Department of Radiology, University of Missouri Columbia, Columbia, MO, USA; 3Laboratório de Biomateriais Poliméricos e Nanotheranóstica, Centro de Química e Meio Ambiente, Instituto de Pesquisas Energéticas e Nucleares (IPEN), Comissão Nacional de Energia Nuclear- IPEN/CNEN-SP, São Paulo, SP, Brazil; 4Laboratório de Ecotoxicologia, Companhia Ambiental do Estado de São Paulo (CETESB), São Paulo, SP, Brazil; 5Dhanvantari Nano Ayushadi Pvt Ltd, Chennai, Tamil Nadu, IndiaCorrespondence: Kattesh V Katti; Ademar B Lugão Email kattik@health.missouri.edu; ablugao@gmail.comIntroduction: We report, herein, in vitro, and in vivo toxicity evaluation of silver nanoparticles stabilized with gum arabic protein (AgNP-GP) in Daphnia similis, Danio rerio embryos and in Sprague Dawley rats.Purpose: The objective of this investigation was to evaluate in vitro and in vivo toxicity of silver nanoparticles stabilized with gum arabic protein (AgNP-GP), in multispecies due to the recognition that toxicity evaluations beyond a single species reflect the environmental realism. In the present study, AgNP-GP was synthesized through the reduction of silver salt using the tri-alanine-phosphine peptide (commonly referred to as “Katti Peptide”) and stabilized using gum arabic protein.Methods: In vitro cytotoxicity tests were performed according to ISO 10993– 5 protocols to assess cytotoxicity index (IC50) values. Acute ecotoxicity (EC50) studies were performed using Daphnia similis, according to the ABNT NBR 15088 protocols. In vivo toxicity also included evaluation of acute embryotoxicity using Danio rerio (zebrafish) embryos following the OECD No. 236 guidelines. We also used Sprague Dawley rats to assess the toxicity of AgNP-GP in doses from 2.5 to 10.0 mg kg− 1 body weight.Results: AgNP-GP nanoparticles were characterized through UV (405 nm), core size (20± 5 nm through TEM), hydrodynamic size (70– 80 nm), Zeta (ζ) potential (- 26 mV) using DLS and Powder X ray diffraction (PXRD) and EDS. PXRD showed pattern consistent with the Ag (1 1 1) peak. EC50 in Daphnia similis was 4.40 (3.59– 5.40) μg L− 1. In the zebrafish species, LC50 was 177 μg L− 1. Oral administration of AgNP-GP in Sprague Dawley rats for a period of 28 days revealed no adverse effects in doses of up to 10.0 mg kg− 1 b.w. in both male and female animals.Conclusion: The non-toxicity of AgNP-GP in rats offers a myriad of applications of AgNP-GP in health and hygiene for use as antibiotics, antimicrobial and antifungal agents.Keywords: nanoparticles, zebrafish, cytotoxicity, ecotoxicity, Daphnia similis, Sprague DawleyMaziero JSThipe VCRogero SOCavalcante AKDamasceno KCOrmenio MBMartini GABatista JGSViveiros WKatti KKRaphael Karikachery ADhurvas Mohandoss DDhurvas RDNappinnai MRogero JRLugão ABKatti KVDove Medical Pressarticlenanoparticleszebrafishcytotoxicityecotoxicitydaphnia similissprague dawley.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 7359-7376 (2020)

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