Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora

Abstract The existence and function of neurons remain largely unexplored in scleractinian corals. To gain a better understanding of neuronal functions in coral physiology, this study focused on Glycine-Leucine-Tryptophan-amide family neuropeptides (GLWamides), which have been shown to induce muscle...

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Autores principales:	Shinya Shikina, Yi-Ling Chiu, Yan Zhang, Yi-ChenYao, Tai-Yu Liu, Pin-Hsuan Tsai, Céline Zatylny-Gaudin, Ching-Fong Chang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/916aa739dff248008eaf28d41aae3d96
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spelling	oai:doaj.org-article:916aa739dff248008eaf28d41aae3d962021-12-02T17:52:42ZInvolvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora10.1038/s41598-020-66438-32045-2322https://doaj.org/article/916aa739dff248008eaf28d41aae3d962020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66438-3https://doaj.org/toc/2045-2322Abstract The existence and function of neurons remain largely unexplored in scleractinian corals. To gain a better understanding of neuronal functions in coral physiology, this study focused on Glycine-Leucine-Tryptophan-amide family neuropeptides (GLWamides), which have been shown to induce muscle contraction and larval metamorphosis in other cnidarians. Molecular identification and functional characterization of GLWamides in the adult stony coral Euphyllia ancora were performed. We successfully elucidated the full-length cDNA of GLWamide preprohormone in E. ancora (named EaGLW preprohormone). The deduced amino acid sequence was predicted to contain six potential GLWamide peptides. Tissue distribution analysis demonstrated that transcripts of EaGLW preprohormone were mainly expressed in the mouth (including the pharynx) and tentacles of the polyps. Immunodetection with an anti-GLWamide monoclonal antibody revealed that GLWamide neurons were mainly distributed in the epidermis of the mouth region and tentacle, in agreement with the distribution patterns of the transcripts. Treatment of the isolated mouth and tentacles with synthetic GLWamide peptides induced the contraction of these isolated tissues. Treatment of polyps with synthetic GLWamide peptides induced the contraction of polyps. These results suggest that GLWamides are involved in polyp contraction (myoactivity) in adult scleractinians. Our data provide new information on the physiological function of neuropeptides in scleractinians.Shinya ShikinaYi-Ling ChiuYan ZhangYi-ChenYaoTai-Yu LiuPin-Hsuan TsaiCéline Zatylny-GaudinChing-Fong ChangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shinya Shikina Yi-Ling Chiu Yan Zhang Yi-ChenYao Tai-Yu Liu Pin-Hsuan Tsai Céline Zatylny-Gaudin Ching-Fong Chang Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora
description	Abstract The existence and function of neurons remain largely unexplored in scleractinian corals. To gain a better understanding of neuronal functions in coral physiology, this study focused on Glycine-Leucine-Tryptophan-amide family neuropeptides (GLWamides), which have been shown to induce muscle contraction and larval metamorphosis in other cnidarians. Molecular identification and functional characterization of GLWamides in the adult stony coral Euphyllia ancora were performed. We successfully elucidated the full-length cDNA of GLWamide preprohormone in E. ancora (named EaGLW preprohormone). The deduced amino acid sequence was predicted to contain six potential GLWamide peptides. Tissue distribution analysis demonstrated that transcripts of EaGLW preprohormone were mainly expressed in the mouth (including the pharynx) and tentacles of the polyps. Immunodetection with an anti-GLWamide monoclonal antibody revealed that GLWamide neurons were mainly distributed in the epidermis of the mouth region and tentacle, in agreement with the distribution patterns of the transcripts. Treatment of the isolated mouth and tentacles with synthetic GLWamide peptides induced the contraction of these isolated tissues. Treatment of polyps with synthetic GLWamide peptides induced the contraction of polyps. These results suggest that GLWamides are involved in polyp contraction (myoactivity) in adult scleractinians. Our data provide new information on the physiological function of neuropeptides in scleractinians.
format	article
author	Shinya Shikina Yi-Ling Chiu Yan Zhang Yi-ChenYao Tai-Yu Liu Pin-Hsuan Tsai Céline Zatylny-Gaudin Ching-Fong Chang
author_facet	Shinya Shikina Yi-Ling Chiu Yan Zhang Yi-ChenYao Tai-Yu Liu Pin-Hsuan Tsai Céline Zatylny-Gaudin Ching-Fong Chang
author_sort	Shinya Shikina
title	Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora
title_short	Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora
title_full	Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora
title_fullStr	Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora
title_full_unstemmed	Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora
title_sort	involvement of glwamide neuropeptides in polyp contraction of the adult stony coral euphyllia ancora
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/916aa739dff248008eaf28d41aae3d96
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Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora

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