Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses

With the outbreak of the recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019, coronaviruses have become a global research hotspot in the field of virology. Coronaviruses mainly cause respiratory and digestive tract diseases, several coronaviruses are responsible for porcine d...

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Autores principales:	Zhongzhou Pan, Jiaxuan Lu, Ningning Wang, Wan-Ting He, Letian Zhang, Wen Zhao, Shuo Su
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	real-time pcr swine diarrhea virus coronavirus detection Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/91712ce7612244f7b8928da0fac1ea4f
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spelling	oai:doaj.org-article:91712ce7612244f7b8928da0fac1ea4f2021-11-17T14:21:58ZDevelopment of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses2150-55942150-560810.1080/21505594.2020.1771980https://doaj.org/article/91712ce7612244f7b8928da0fac1ea4f2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1771980https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608With the outbreak of the recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019, coronaviruses have become a global research hotspot in the field of virology. Coronaviruses mainly cause respiratory and digestive tract diseases, several coronaviruses are responsible for porcine diarrhea, such as porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and emerging swine acute diarrhea syndrome coronavirus (SADS-CoV). Those viruses have caused huge economic losses and are considered as potential public health threats. Porcine torovirus (PToV) and coronaviruses, sharing similar genomic structure and replication strategy, belong to the same order Nidovirales. Here, we developed a multiplex TaqMan-probe-based real-time PCR for the simultaneous detection of PEDV, PDCoV, PToV, and SADS-CoV for the first time. Specific primers and TaqMan fluorescent probes were designed targeting the ORF1a region of PDEV, PToV, and SADS-CoV and the ORF1b region of PDCoV. The method showed high sensitivity and specificity, with a detection limit of 1 × 102 copies/μL for each pathogen. A total of 101 clinical swine samples with signs of diarrhea were analyzed using this method, and the result showed good consistency with conventional reverse transcription PCR (RT-PCR). This method improves the efficiency for surveillance of these emerging and reemerging swine enteric viruses and can help reduce economic losses to the pig industry, which also benefits animal and public health.Zhongzhou PanJiaxuan LuNingning WangWan-Ting HeLetian ZhangWen ZhaoShuo SuTaylor & Francis Grouparticlereal-time pcrswine diarrhea viruscoronavirusdetectionInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 707-718 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	real-time pcr swine diarrhea virus coronavirus detection Infectious and parasitic diseases RC109-216
spellingShingle	real-time pcr swine diarrhea virus coronavirus detection Infectious and parasitic diseases RC109-216 Zhongzhou Pan Jiaxuan Lu Ningning Wang Wan-Ting He Letian Zhang Wen Zhao Shuo Su Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses
description	With the outbreak of the recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019, coronaviruses have become a global research hotspot in the field of virology. Coronaviruses mainly cause respiratory and digestive tract diseases, several coronaviruses are responsible for porcine diarrhea, such as porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and emerging swine acute diarrhea syndrome coronavirus (SADS-CoV). Those viruses have caused huge economic losses and are considered as potential public health threats. Porcine torovirus (PToV) and coronaviruses, sharing similar genomic structure and replication strategy, belong to the same order Nidovirales. Here, we developed a multiplex TaqMan-probe-based real-time PCR for the simultaneous detection of PEDV, PDCoV, PToV, and SADS-CoV for the first time. Specific primers and TaqMan fluorescent probes were designed targeting the ORF1a region of PDEV, PToV, and SADS-CoV and the ORF1b region of PDCoV. The method showed high sensitivity and specificity, with a detection limit of 1 × 102 copies/μL for each pathogen. A total of 101 clinical swine samples with signs of diarrhea were analyzed using this method, and the result showed good consistency with conventional reverse transcription PCR (RT-PCR). This method improves the efficiency for surveillance of these emerging and reemerging swine enteric viruses and can help reduce economic losses to the pig industry, which also benefits animal and public health.
format	article
author	Zhongzhou Pan Jiaxuan Lu Ningning Wang Wan-Ting He Letian Zhang Wen Zhao Shuo Su
author_facet	Zhongzhou Pan Jiaxuan Lu Ningning Wang Wan-Ting He Letian Zhang Wen Zhao Shuo Su
author_sort	Zhongzhou Pan
title	Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses
title_short	Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses
title_full	Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses
title_fullStr	Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses
title_full_unstemmed	Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses
title_sort	development of a taqman-probe-based multiplex real-time pcr for the simultaneous detection of emerging and reemerging swine coronaviruses
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/91712ce7612244f7b8928da0fac1ea4f
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Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses

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