LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion

Abstract The mechanism of action of various viruses has been the primary focus of many studies. Yet, the data on RNA modifications in any type of virus are scarce. Methods for the sensitive analysis of RNA modifications have been developed only recently and they have not been applied to viruses. In...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anna Šimonová, Barbora Svojanovská, Jana Trylčová, Martin Hubálek, Ondřej Moravčík, Martin Zavřel, Marcela Pávová, Jan Hodek, Jan Weber, Josef Cvačka, Jan Pačes, Hana Cahová
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/91713de77eb849e6b8dc30726e6d9355
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:91713de77eb849e6b8dc30726e6d9355
record_format	dspace
spelling	oai:doaj.org-article:91713de77eb849e6b8dc30726e6d93552021-12-02T15:09:31ZLC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion10.1038/s41598-019-45079-12045-2322https://doaj.org/article/91713de77eb849e6b8dc30726e6d93552019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45079-1https://doaj.org/toc/2045-2322Abstract The mechanism of action of various viruses has been the primary focus of many studies. Yet, the data on RNA modifications in any type of virus are scarce. Methods for the sensitive analysis of RNA modifications have been developed only recently and they have not been applied to viruses. In particular, the RNA composition of HIV-1 virions has never been determined with sufficiently exact methods. Here, we reveal that the RNA of HIV-1 virions contains surprisingly high amount of the 1-methyladenosine. We are the first to use a liquid chromatography-mass spectrometry analysis (LC/MS) of virion RNA, which we combined with m1A profiling and deep sequencing. We found that m1A was present in the tRNA, but not in the genomic HIV-1 RNA and the abundant 7SL RNA. We were able to calculate that an HIV-1 virion contains per 2 copies of genomic RNA and 14 copies of 7SL RNA also 770 copies of tRNA, which is approximately 10 times more than thus far expected. These new insights into the composition of the HIV-1 virion can help in future studies to identify the role of nonprimer tRNAs in retroviruses. Moreover, we present a promising new tool for studying the compositions of virions.Anna ŠimonováBarbora SvojanovskáJana TrylčováMartin HubálekOndřej MoravčíkMartin ZavřelMarcela PávováJan HodekJan WeberJosef CvačkaJan PačesHana CahováNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Šimonová Barbora Svojanovská Jana Trylčová Martin Hubálek Ondřej Moravčík Martin Zavřel Marcela Pávová Jan Hodek Jan Weber Josef Cvačka Jan Pačes Hana Cahová LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion
description	Abstract The mechanism of action of various viruses has been the primary focus of many studies. Yet, the data on RNA modifications in any type of virus are scarce. Methods for the sensitive analysis of RNA modifications have been developed only recently and they have not been applied to viruses. In particular, the RNA composition of HIV-1 virions has never been determined with sufficiently exact methods. Here, we reveal that the RNA of HIV-1 virions contains surprisingly high amount of the 1-methyladenosine. We are the first to use a liquid chromatography-mass spectrometry analysis (LC/MS) of virion RNA, which we combined with m1A profiling and deep sequencing. We found that m1A was present in the tRNA, but not in the genomic HIV-1 RNA and the abundant 7SL RNA. We were able to calculate that an HIV-1 virion contains per 2 copies of genomic RNA and 14 copies of 7SL RNA also 770 copies of tRNA, which is approximately 10 times more than thus far expected. These new insights into the composition of the HIV-1 virion can help in future studies to identify the role of nonprimer tRNAs in retroviruses. Moreover, we present a promising new tool for studying the compositions of virions.
format	article
author	Anna Šimonová Barbora Svojanovská Jana Trylčová Martin Hubálek Ondřej Moravčík Martin Zavřel Marcela Pávová Jan Hodek Jan Weber Josef Cvačka Jan Pačes Hana Cahová
author_facet	Anna Šimonová Barbora Svojanovská Jana Trylčová Martin Hubálek Ondřej Moravčík Martin Zavřel Marcela Pávová Jan Hodek Jan Weber Josef Cvačka Jan Pačes Hana Cahová
author_sort	Anna Šimonová
title	LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion
title_short	LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion
title_full	LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion
title_fullStr	LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion
title_full_unstemmed	LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion
title_sort	lc/ms analysis and deep sequencing reveal the accurate rna composition in the hiv-1 virion
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/91713de77eb849e6b8dc30726e6d9355
work_keys_str_mv	AT annasimonova lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT barborasvojanovska lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT janatrylcova lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT martinhubalek lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT ondrejmoravcik lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT martinzavrel lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT marcelapavova lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT janhodek lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT janweber lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT josefcvacka lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT janpaces lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion AT hanacahova lcmsanalysisanddeepsequencingrevealtheaccuraternacompositioninthehiv1virion
_version_	1718387828524580864

LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion

Ejemplares similares