High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes

Abstract In vitro high-throughput non-depletive quantitation of chemicals in biofluids is of growing interest in many areas. Some of the challenges facing researchers include the limited volume of biofluids, rapid and high-throughput sampling requirements, and the lack of reliable methods. Coupled t...

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Autores principales:	Ezel Boyacı, Barbara Bojko, Nathaly Reyes-Garcés, Justen J. Poole, Germán Augusto Gómez-Ríos, Alexandre Teixeira, Beate Nicol, Janusz Pawliszyn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9171e418dd804e34b31ece1dafae6cc6
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spelling	oai:doaj.org-article:9171e418dd804e34b31ece1dafae6cc62021-12-02T15:08:48ZHigh-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes10.1038/s41598-018-19313-12045-2322https://doaj.org/article/9171e418dd804e34b31ece1dafae6cc62018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19313-1https://doaj.org/toc/2045-2322Abstract In vitro high-throughput non-depletive quantitation of chemicals in biofluids is of growing interest in many areas. Some of the challenges facing researchers include the limited volume of biofluids, rapid and high-throughput sampling requirements, and the lack of reliable methods. Coupled to the above, growing interest in the monitoring of kinetics and dynamics of miniaturized biosystems has spurred the demand for development of novel and revolutionary methodologies for analysis of biofluids. The applicability of solid-phase microextraction (SPME) is investigated as a potential technology to fulfill the aforementioned requirements. As analytes with sufficient diversity in their physicochemical features, nicotine, N,N-Diethyl-meta-toluamide, and diclofenac were selected as test compounds for the study. The objective was to develop methodologies that would allow repeated non-depletive sampling from 96-well plates, using 100 µL of sample. Initially, thin film-SPME was investigated. Results revealed substantial depletion and consequent disruption in the system. Therefore, new ultra-thin coated fibers were developed. The applicability of this device to the described sampling scenario was tested by determining the protein binding of the analytes. Results showed good agreement with rapid equilibrium dialysis. The presented method allows high-throughput analysis using small volumes, enabling fast reliable free and total concentration determinations without disruption of system equilibrium.Ezel BoyacıBarbara BojkoNathaly Reyes-GarcésJusten J. PooleGermán Augusto Gómez-RíosAlexandre TeixeiraBeate NicolJanusz PawliszynNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ezel Boyacı Barbara Bojko Nathaly Reyes-Garcés Justen J. Poole Germán Augusto Gómez-Ríos Alexandre Teixeira Beate Nicol Janusz Pawliszyn High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes
description	Abstract In vitro high-throughput non-depletive quantitation of chemicals in biofluids is of growing interest in many areas. Some of the challenges facing researchers include the limited volume of biofluids, rapid and high-throughput sampling requirements, and the lack of reliable methods. Coupled to the above, growing interest in the monitoring of kinetics and dynamics of miniaturized biosystems has spurred the demand for development of novel and revolutionary methodologies for analysis of biofluids. The applicability of solid-phase microextraction (SPME) is investigated as a potential technology to fulfill the aforementioned requirements. As analytes with sufficient diversity in their physicochemical features, nicotine, N,N-Diethyl-meta-toluamide, and diclofenac were selected as test compounds for the study. The objective was to develop methodologies that would allow repeated non-depletive sampling from 96-well plates, using 100 µL of sample. Initially, thin film-SPME was investigated. Results revealed substantial depletion and consequent disruption in the system. Therefore, new ultra-thin coated fibers were developed. The applicability of this device to the described sampling scenario was tested by determining the protein binding of the analytes. Results showed good agreement with rapid equilibrium dialysis. The presented method allows high-throughput analysis using small volumes, enabling fast reliable free and total concentration determinations without disruption of system equilibrium.
format	article
author	Ezel Boyacı Barbara Bojko Nathaly Reyes-Garcés Justen J. Poole Germán Augusto Gómez-Ríos Alexandre Teixeira Beate Nicol Janusz Pawliszyn
author_facet	Ezel Boyacı Barbara Bojko Nathaly Reyes-Garcés Justen J. Poole Germán Augusto Gómez-Ríos Alexandre Teixeira Beate Nicol Janusz Pawliszyn
author_sort	Ezel Boyacı
title	High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes
title_short	High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes
title_full	High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes
title_fullStr	High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes
title_full_unstemmed	High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes
title_sort	high-throughput analysis using non-depletive spme: challenges and applications to the determination of free and total concentrations in small sample volumes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9171e418dd804e34b31ece1dafae6cc6
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High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes

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