Effect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry

Platelets are functionally versatile blood cells involved in thrombosis, hemostasis, atherosclerosis, and immune response. Platelet interaction with the immediate microenvironment in blood, vasculature, and tissues alters platelet morphology. The quantification of platelet morphodynamics by geometri...

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Autores principales:	Jan Seifert, Hendrik von Eysmondt, Madhumita Chatterjee, Meinrad Gawaz, Tilman E. Schäffer
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	platelet deep learning neural network oxLDL platelet shape platelet migration Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9177a910c501435c9903ea692d1e550f
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spelling	oai:doaj.org-article:9177a910c501435c9903ea692d1e550f2021-11-25T17:09:11ZEffect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry10.3390/cells101129322073-4409https://doaj.org/article/9177a910c501435c9903ea692d1e550f2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2932https://doaj.org/toc/2073-4409Platelets are functionally versatile blood cells involved in thrombosis, hemostasis, atherosclerosis, and immune response. Platelet interaction with the immediate microenvironment in blood, vasculature, and tissues alters platelet morphology. The quantification of platelet morphodynamics by geometrical parameters (morphometry) can provide important insights into how platelets sense and respond to stimulatory cues in their vicinity. However, the extraction of platelet shapes from phase contrast microscopy images by conventional image processing is difficult. Here, we used a convolutional neural network (CNN) to develop a deep-learning-based approach for the unbiased extraction of information on platelet morphodynamics by phase contrast microscopy. We then investigated the effect of normal and oxidized low-density lipoproteins (LDL, oxLDL) on platelet morphodynamics, spreading, and haptotactic migration. Exposure of platelets to oxLDL led to a decreased spreading area and rate on fibrinogen, accompanied by increased formation of filopodia and impaired formation of lamellipodia. Haptotactic platelet migration was affected by both LDL and oxLDL in terms of decreased migration velocity and reduced directional persistence. Our results demonstrate the use of deep learning in investigating platelet morphodynamics and reveal differential effects of LDL and oxLDL on platelet morphology and platelet–matrix interaction.Jan SeifertHendrik von EysmondtMadhumita ChatterjeeMeinrad GawazTilman E. SchäfferMDPI AGarticleplateletdeep learningneural networkoxLDLplatelet shapeplatelet migrationBiology (General)QH301-705.5ENCells, Vol 10, Iss 2932, p 2932 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	platelet deep learning neural network oxLDL platelet shape platelet migration Biology (General) QH301-705.5
spellingShingle	platelet deep learning neural network oxLDL platelet shape platelet migration Biology (General) QH301-705.5 Jan Seifert Hendrik von Eysmondt Madhumita Chatterjee Meinrad Gawaz Tilman E. Schäffer Effect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry
description	Platelets are functionally versatile blood cells involved in thrombosis, hemostasis, atherosclerosis, and immune response. Platelet interaction with the immediate microenvironment in blood, vasculature, and tissues alters platelet morphology. The quantification of platelet morphodynamics by geometrical parameters (morphometry) can provide important insights into how platelets sense and respond to stimulatory cues in their vicinity. However, the extraction of platelet shapes from phase contrast microscopy images by conventional image processing is difficult. Here, we used a convolutional neural network (CNN) to develop a deep-learning-based approach for the unbiased extraction of information on platelet morphodynamics by phase contrast microscopy. We then investigated the effect of normal and oxidized low-density lipoproteins (LDL, oxLDL) on platelet morphodynamics, spreading, and haptotactic migration. Exposure of platelets to oxLDL led to a decreased spreading area and rate on fibrinogen, accompanied by increased formation of filopodia and impaired formation of lamellipodia. Haptotactic platelet migration was affected by both LDL and oxLDL in terms of decreased migration velocity and reduced directional persistence. Our results demonstrate the use of deep learning in investigating platelet morphodynamics and reveal differential effects of LDL and oxLDL on platelet morphology and platelet–matrix interaction.
format	article
author	Jan Seifert Hendrik von Eysmondt Madhumita Chatterjee Meinrad Gawaz Tilman E. Schäffer
author_facet	Jan Seifert Hendrik von Eysmondt Madhumita Chatterjee Meinrad Gawaz Tilman E. Schäffer
author_sort	Jan Seifert
title	Effect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry
title_short	Effect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry
title_full	Effect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry
title_fullStr	Effect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry
title_full_unstemmed	Effect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry
title_sort	effect of oxidized ldl on platelet shape, spreading, and migration investigated with deep learning platelet morphometry
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9177a910c501435c9903ea692d1e550f
work_keys_str_mv	AT janseifert effectofoxidizedldlonplateletshapespreadingandmigrationinvestigatedwithdeeplearningplateletmorphometry AT hendrikvoneysmondt effectofoxidizedldlonplateletshapespreadingandmigrationinvestigatedwithdeeplearningplateletmorphometry AT madhumitachatterjee effectofoxidizedldlonplateletshapespreadingandmigrationinvestigatedwithdeeplearningplateletmorphometry AT meinradgawaz effectofoxidizedldlonplateletshapespreadingandmigrationinvestigatedwithdeeplearningplateletmorphometry AT tilmaneschaffer effectofoxidizedldlonplateletshapespreadingandmigrationinvestigatedwithdeeplearningplateletmorphometry
_version_	1718412665213157376

Effect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry

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