The regulation landscape of MAPK signaling cascade for thwarting Bacillus thuringiensis infection in an insect host.

Host-pathogen interactions are central components of ecological networks where the MAPK signaling pathways act as central hubs of these complex interactions. We have previously shown that an insect hormone modulated MAPK signaling cascade participates as a general switch to trans-regulate differenti...

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Autores principales:	Zhaojiang Guo, Shi Kang, Qingjun Wu, Shaoli Wang, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Youjun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9181f4957e07491a94e3410dc99edce7
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spelling	oai:doaj.org-article:9181f4957e07491a94e3410dc99edce72021-12-02T20:00:13ZThe regulation landscape of MAPK signaling cascade for thwarting Bacillus thuringiensis infection in an insect host.1553-73661553-737410.1371/journal.ppat.1009917https://doaj.org/article/9181f4957e07491a94e3410dc99edce72021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009917https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Host-pathogen interactions are central components of ecological networks where the MAPK signaling pathways act as central hubs of these complex interactions. We have previously shown that an insect hormone modulated MAPK signaling cascade participates as a general switch to trans-regulate differential expression of diverse midgut genes in the diamondback moth, Plutella xylostella (L.) to cope with the insecticidal action of Cry1Ac toxin, produced by the entomopathogenic bacterium Bacillus thuringiensis (Bt). The relationship between topology and functions of this four-tiered phosphorylation signaling cascade, however, is an uncharted territory. Here, we carried out a genome-wide characterization of all the MAPK orthologs in P. xylostella to define their phylogenetic relationships and to confirm their evolutionary conserved modules. Results from quantitative phosphoproteomic analyses, combined with functional validations studies using specific inhibitors and dsRNAs lead us to establish a MAPK "road map", where p38 and ERK MAPK signaling pathways, in large part, mount a resistance response against Bt toxins through regulating the differential expression of multiple Cry toxin receptors and their non-receptor paralogs in P. xylostella midgut. These data not only advance our understanding of host-pathogen interactions in agricultural pests, but also inform the future development of biopesticides that could suppress Cry resistance phenotypes.Zhaojiang GuoShi KangQingjun WuShaoli WangNeil CrickmoreXuguo ZhouAlejandra BravoMario SoberónYoujun ZhangPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 9, p e1009917 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Zhaojiang Guo Shi Kang Qingjun Wu Shaoli Wang Neil Crickmore Xuguo Zhou Alejandra Bravo Mario Soberón Youjun Zhang The regulation landscape of MAPK signaling cascade for thwarting Bacillus thuringiensis infection in an insect host.
description	Host-pathogen interactions are central components of ecological networks where the MAPK signaling pathways act as central hubs of these complex interactions. We have previously shown that an insect hormone modulated MAPK signaling cascade participates as a general switch to trans-regulate differential expression of diverse midgut genes in the diamondback moth, Plutella xylostella (L.) to cope with the insecticidal action of Cry1Ac toxin, produced by the entomopathogenic bacterium Bacillus thuringiensis (Bt). The relationship between topology and functions of this four-tiered phosphorylation signaling cascade, however, is an uncharted territory. Here, we carried out a genome-wide characterization of all the MAPK orthologs in P. xylostella to define their phylogenetic relationships and to confirm their evolutionary conserved modules. Results from quantitative phosphoproteomic analyses, combined with functional validations studies using specific inhibitors and dsRNAs lead us to establish a MAPK "road map", where p38 and ERK MAPK signaling pathways, in large part, mount a resistance response against Bt toxins through regulating the differential expression of multiple Cry toxin receptors and their non-receptor paralogs in P. xylostella midgut. These data not only advance our understanding of host-pathogen interactions in agricultural pests, but also inform the future development of biopesticides that could suppress Cry resistance phenotypes.
format	article
author	Zhaojiang Guo Shi Kang Qingjun Wu Shaoli Wang Neil Crickmore Xuguo Zhou Alejandra Bravo Mario Soberón Youjun Zhang
author_facet	Zhaojiang Guo Shi Kang Qingjun Wu Shaoli Wang Neil Crickmore Xuguo Zhou Alejandra Bravo Mario Soberón Youjun Zhang
author_sort	Zhaojiang Guo
title	The regulation landscape of MAPK signaling cascade for thwarting Bacillus thuringiensis infection in an insect host.
title_short	The regulation landscape of MAPK signaling cascade for thwarting Bacillus thuringiensis infection in an insect host.
title_full	The regulation landscape of MAPK signaling cascade for thwarting Bacillus thuringiensis infection in an insect host.
title_fullStr	The regulation landscape of MAPK signaling cascade for thwarting Bacillus thuringiensis infection in an insect host.
title_full_unstemmed	The regulation landscape of MAPK signaling cascade for thwarting Bacillus thuringiensis infection in an insect host.
title_sort	regulation landscape of mapk signaling cascade for thwarting bacillus thuringiensis infection in an insect host.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9181f4957e07491a94e3410dc99edce7
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The regulation landscape of MAPK signaling cascade for thwarting Bacillus thuringiensis infection in an insect host.

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