Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters

Antibiotic persistence is a phenomenon in which rare cells of a clonal bacterial population can survive antibiotic doses that kill their kin, even though the entire population is genetically susceptible. With antibiotic treatment failure on the rise, there is growing interest in understanding the mo...

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Autores principales:	Patricia J. Hare, Travis J. LaGree, Brandon A. Byrd, Angela M. DeMarco, Wendy W. K. Mok
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibiotic persistence phenotypic heterogeneity single-cell analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/91836513ab7f47aa9f892028e37d147b
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spelling	oai:doaj.org-article:91836513ab7f47aa9f892028e37d147b2021-11-25T18:24:47ZSingle-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters10.3390/microorganisms91122772076-2607https://doaj.org/article/91836513ab7f47aa9f892028e37d147b2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2277https://doaj.org/toc/2076-2607Antibiotic persistence is a phenomenon in which rare cells of a clonal bacterial population can survive antibiotic doses that kill their kin, even though the entire population is genetically susceptible. With antibiotic treatment failure on the rise, there is growing interest in understanding the molecular mechanisms underlying bacterial phenotypic heterogeneity and antibiotic persistence. However, elucidating these rare cell states can be technically challenging. The advent of single-cell techniques has enabled us to observe and quantitatively investigate individual cells in complex, phenotypically heterogeneous populations. In this review, we will discuss current technologies for studying persister phenotypes, including fluorescent tags and biosensors used to elucidate cellular processes; advances in flow cytometry, mass spectrometry, Raman spectroscopy, and microfluidics that contribute high-throughput and high-content information; and next-generation sequencing for powerful insights into genetic and transcriptomic programs. We will further discuss existing knowledge gaps, cutting-edge technologies that can address them, and how advances in single-cell microbiology can potentially improve infectious disease treatment outcomes.Patricia J. HareTravis J. LaGreeBrandon A. ByrdAngela M. DeMarcoWendy W. K. MokMDPI AGarticleantibiotic persistencephenotypic heterogeneitysingle-cell analysisBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2277, p 2277 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic persistence phenotypic heterogeneity single-cell analysis Biology (General) QH301-705.5
spellingShingle	antibiotic persistence phenotypic heterogeneity single-cell analysis Biology (General) QH301-705.5 Patricia J. Hare Travis J. LaGree Brandon A. Byrd Angela M. DeMarco Wendy W. K. Mok Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters
description	Antibiotic persistence is a phenomenon in which rare cells of a clonal bacterial population can survive antibiotic doses that kill their kin, even though the entire population is genetically susceptible. With antibiotic treatment failure on the rise, there is growing interest in understanding the molecular mechanisms underlying bacterial phenotypic heterogeneity and antibiotic persistence. However, elucidating these rare cell states can be technically challenging. The advent of single-cell techniques has enabled us to observe and quantitatively investigate individual cells in complex, phenotypically heterogeneous populations. In this review, we will discuss current technologies for studying persister phenotypes, including fluorescent tags and biosensors used to elucidate cellular processes; advances in flow cytometry, mass spectrometry, Raman spectroscopy, and microfluidics that contribute high-throughput and high-content information; and next-generation sequencing for powerful insights into genetic and transcriptomic programs. We will further discuss existing knowledge gaps, cutting-edge technologies that can address them, and how advances in single-cell microbiology can potentially improve infectious disease treatment outcomes.
format	article
author	Patricia J. Hare Travis J. LaGree Brandon A. Byrd Angela M. DeMarco Wendy W. K. Mok
author_facet	Patricia J. Hare Travis J. LaGree Brandon A. Byrd Angela M. DeMarco Wendy W. K. Mok
author_sort	Patricia J. Hare
title	Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters
title_short	Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters
title_full	Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters
title_fullStr	Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters
title_full_unstemmed	Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters
title_sort	single-cell technologies to study phenotypic heterogeneity and bacterial persisters
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/91836513ab7f47aa9f892028e37d147b
work_keys_str_mv	AT patriciajhare singlecelltechnologiestostudyphenotypicheterogeneityandbacterialpersisters AT travisjlagree singlecelltechnologiestostudyphenotypicheterogeneityandbacterialpersisters AT brandonabyrd singlecelltechnologiestostudyphenotypicheterogeneityandbacterialpersisters AT angelamdemarco singlecelltechnologiestostudyphenotypicheterogeneityandbacterialpersisters AT wendywkmok singlecelltechnologiestostudyphenotypicheterogeneityandbacterialpersisters
_version_	1718411212129042432

Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters

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