Genome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance

Abiotic stresses are increasingly harmful to crop yield and quality. Calcium and its signaling pathway play an important role in modulating plant stress tolerance. As specific Ca<sup>2+</sup> sensors, calcineurin B-like (CBL) proteins play vital roles in plant stress response and calcium...

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Autores principales:	Peihong Chen, Jie Yang, Quanlin Mei, Huayu Liu, Yunpeng Cheng, Fengwang Ma, Ke Mao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Malus domestica</i> calcium calcineurin B-like proteins Na<sup>+</sup> accumulation salt tolerance Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/9183f5e8f11345d7bec23ebb0e35f782
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spelling	oai:doaj.org-article:9183f5e8f11345d7bec23ebb0e35f7822021-11-25T17:56:33ZGenome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance10.3390/ijms2222124301422-00671661-6596https://doaj.org/article/9183f5e8f11345d7bec23ebb0e35f7822021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12430https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Abiotic stresses are increasingly harmful to crop yield and quality. Calcium and its signaling pathway play an important role in modulating plant stress tolerance. As specific Ca<sup>2+</sup> sensors, calcineurin B-like (CBL) proteins play vital roles in plant stress response and calcium signaling. The CBL family has been identified in many plant species; however, the characterization of the CBL family and the functional study of apple MdCBL proteins in salt response have yet to be conducted in apple. In this study, 11 <i>MdCBL</i> genes were identified from the apple genome. The coding sequences of these <i>MdCBL</i> genes were cloned, and the gene structure and conserved motifs were analyzed in detail. The phylogenetic analysis indicated that these MdCBL proteins could be divided into four groups. The functional identification in Na<sup>+</sup>-sensitive yeast mutant showed that the overexpression of seven <i>MdCBL</i> genes could confer enhanced salt stress resistance in transgenic yeast. The function of <i>MdCBL10.1</i> in regulating salt tolerance was also verified in cisgenic apple calli and apple plants. These results provided valuable insights for future research examining the function and mechanism of CBL proteins in regulating apple salt tolerance.Peihong ChenJie YangQuanlin MeiHuayu LiuYunpeng ChengFengwang MaKe MaoMDPI AGarticle<i>Malus domestica</i>calciumcalcineurin B-like proteinsNa<sup>+</sup> accumulationsalt toleranceBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12430, p 12430 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Malus domestica</i> calcium calcineurin B-like proteins Na<sup>+</sup> accumulation salt tolerance Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Malus domestica</i> calcium calcineurin B-like proteins Na<sup>+</sup> accumulation salt tolerance Biology (General) QH301-705.5 Chemistry QD1-999 Peihong Chen Jie Yang Quanlin Mei Huayu Liu Yunpeng Cheng Fengwang Ma Ke Mao Genome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance
description	Abiotic stresses are increasingly harmful to crop yield and quality. Calcium and its signaling pathway play an important role in modulating plant stress tolerance. As specific Ca<sup>2+</sup> sensors, calcineurin B-like (CBL) proteins play vital roles in plant stress response and calcium signaling. The CBL family has been identified in many plant species; however, the characterization of the CBL family and the functional study of apple MdCBL proteins in salt response have yet to be conducted in apple. In this study, 11 <i>MdCBL</i> genes were identified from the apple genome. The coding sequences of these <i>MdCBL</i> genes were cloned, and the gene structure and conserved motifs were analyzed in detail. The phylogenetic analysis indicated that these MdCBL proteins could be divided into four groups. The functional identification in Na<sup>+</sup>-sensitive yeast mutant showed that the overexpression of seven <i>MdCBL</i> genes could confer enhanced salt stress resistance in transgenic yeast. The function of <i>MdCBL10.1</i> in regulating salt tolerance was also verified in cisgenic apple calli and apple plants. These results provided valuable insights for future research examining the function and mechanism of CBL proteins in regulating apple salt tolerance.
format	article
author	Peihong Chen Jie Yang Quanlin Mei Huayu Liu Yunpeng Cheng Fengwang Ma Ke Mao
author_facet	Peihong Chen Jie Yang Quanlin Mei Huayu Liu Yunpeng Cheng Fengwang Ma Ke Mao
author_sort	Peihong Chen
title	Genome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance
title_short	Genome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance
title_full	Genome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance
title_fullStr	Genome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance
title_full_unstemmed	Genome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance
title_sort	genome-wide analysis of the apple cbl family reveals that mdcbl10.1 functions positively in modulating apple salt tolerance
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9183f5e8f11345d7bec23ebb0e35f782
work_keys_str_mv	AT peihongchen genomewideanalysisoftheapplecblfamilyrevealsthatmdcbl101functionspositivelyinmodulatingapplesalttolerance AT jieyang genomewideanalysisoftheapplecblfamilyrevealsthatmdcbl101functionspositivelyinmodulatingapplesalttolerance AT quanlinmei genomewideanalysisoftheapplecblfamilyrevealsthatmdcbl101functionspositivelyinmodulatingapplesalttolerance AT huayuliu genomewideanalysisoftheapplecblfamilyrevealsthatmdcbl101functionspositivelyinmodulatingapplesalttolerance AT yunpengcheng genomewideanalysisoftheapplecblfamilyrevealsthatmdcbl101functionspositivelyinmodulatingapplesalttolerance AT fengwangma genomewideanalysisoftheapplecblfamilyrevealsthatmdcbl101functionspositivelyinmodulatingapplesalttolerance AT kemao genomewideanalysisoftheapplecblfamilyrevealsthatmdcbl101functionspositivelyinmodulatingapplesalttolerance
_version_	1718411816492597248

Genome-Wide Analysis of the Apple CBL Family Reveals That Mdcbl10.1 Functions Positively in Modulating Apple Salt Tolerance

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