The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear

Cholesteatoma of the middle ear is a kind of cystic disease with clear boundary formed by the abnormal growth of keratosquamous epithelium in temporal bone. Cholesteatoma otitis caused by it is a common disease in otorhinolaryngology. The EPR effect promotes the selective distribution of macromolecu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hongwei Zheng, Wenlun Wang, Shichang Li, Lin Han
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	nano delivery vector cholesteatoma in middle ear of children apoptosis pten/pi3k/akt Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/91874ce7f8584ec7a8c7b827836a1e57
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:91874ce7f8584ec7a8c7b827836a1e57
record_format	dspace
spelling	oai:doaj.org-article:91874ce7f8584ec7a8c7b827836a1e572021-11-04T15:51:53ZThe Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear2165-59792165-598710.1080/21655979.2021.1984718https://doaj.org/article/91874ce7f8584ec7a8c7b827836a1e572021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1984718https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Cholesteatoma of the middle ear is a kind of cystic disease with clear boundary formed by the abnormal growth of keratosquamous epithelium in temporal bone. Cholesteatoma otitis caused by it is a common disease in otorhinolaryngology. The EPR effect promotes the selective distribution of macromolecular substances in tumor tissues, which can increase drug efficacy. The purpose of this paper is to prepare and deliver the mir34a small molecule regulator, rubine, by nanotechnology, and to deliver it to the cells successfully. It can passively target tumor tissue through EPR effect, and play its regulatory role on miR-34a, thus inhibiting the growth of cholesteatoma cells. The effects of nano delivery on apoptosis and PIEN/P13K/AKt of children with middle ear choledochoma were tested in this paper. The experimental results were conducted on cholesteatoma cells as cell lines and balb/c nude mice as experimental objects. The expression of PTEN/PI3K/AKT in experimental group and control group was detected by immunohistochemistry. Apoptosis was discussed by cell activity detection. The physical and chemical properties, encapsulation efficiency, drug release ability in vitro and antitumor activity of nanoparticles in vitro and in vivo were studied. The results of cell level experiments in vitro showed that free RUBINE caused about 15% apoptosis, which was not different from RC NPs. The results showed that the nanoparticles could improve the expression of miR-34 in the cells, and then regulate the expression of Bcl-2, Cdk6 and CyclinD1, and play the inhibitory effect of miR-34a on the proliferation and migration of tumor cells.Hongwei ZhengWenlun WangShichang LiLin HanTaylor & Francis Grouparticlenano delivery vectorcholesteatoma in middle ear of childrenapoptosispten/pi3k/aktBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8809-8821 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nano delivery vector cholesteatoma in middle ear of children apoptosis pten/pi3k/akt Biotechnology TP248.13-248.65
spellingShingle	nano delivery vector cholesteatoma in middle ear of children apoptosis pten/pi3k/akt Biotechnology TP248.13-248.65 Hongwei Zheng Wenlun Wang Shichang Li Lin Han The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear
description	Cholesteatoma of the middle ear is a kind of cystic disease with clear boundary formed by the abnormal growth of keratosquamous epithelium in temporal bone. Cholesteatoma otitis caused by it is a common disease in otorhinolaryngology. The EPR effect promotes the selective distribution of macromolecular substances in tumor tissues, which can increase drug efficacy. The purpose of this paper is to prepare and deliver the mir34a small molecule regulator, rubine, by nanotechnology, and to deliver it to the cells successfully. It can passively target tumor tissue through EPR effect, and play its regulatory role on miR-34a, thus inhibiting the growth of cholesteatoma cells. The effects of nano delivery on apoptosis and PIEN/P13K/AKt of children with middle ear choledochoma were tested in this paper. The experimental results were conducted on cholesteatoma cells as cell lines and balb/c nude mice as experimental objects. The expression of PTEN/PI3K/AKT in experimental group and control group was detected by immunohistochemistry. Apoptosis was discussed by cell activity detection. The physical and chemical properties, encapsulation efficiency, drug release ability in vitro and antitumor activity of nanoparticles in vitro and in vivo were studied. The results of cell level experiments in vitro showed that free RUBINE caused about 15% apoptosis, which was not different from RC NPs. The results showed that the nanoparticles could improve the expression of miR-34 in the cells, and then regulate the expression of Bcl-2, Cdk6 and CyclinD1, and play the inhibitory effect of miR-34a on the proliferation and migration of tumor cells.
format	article
author	Hongwei Zheng Wenlun Wang Shichang Li Lin Han
author_facet	Hongwei Zheng Wenlun Wang Shichang Li Lin Han
author_sort	Hongwei Zheng
title	The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear
title_short	The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear
title_full	The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear
title_fullStr	The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear
title_full_unstemmed	The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear
title_sort	effect of zbxz23ir-21 nano(nanomaterials) delivery vector on apoptosis and pten(phosphatase and tensin homolog deleted on chromosome ten)/pi3k(intracellular phosphatidylinositol kinase)/akt(related to the a and c kinase) in children with cholesteatoma in middle ear
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/91874ce7f8584ec7a8c7b827836a1e57
work_keys_str_mv	AT hongweizheng theeffectofzbxz23ir21nanonanomaterialsdeliveryvectoronapoptosisandptenphosphataseandtensinhomologdeletedonchromosometenpi3kintracellularphosphatidylinositolkinaseaktrelatedtotheaandckinaseinchildrenwithcholesteatomainmiddleear AT wenlunwang theeffectofzbxz23ir21nanonanomaterialsdeliveryvectoronapoptosisandptenphosphataseandtensinhomologdeletedonchromosometenpi3kintracellularphosphatidylinositolkinaseaktrelatedtotheaandckinaseinchildrenwithcholesteatomainmiddleear AT shichangli theeffectofzbxz23ir21nanonanomaterialsdeliveryvectoronapoptosisandptenphosphataseandtensinhomologdeletedonchromosometenpi3kintracellularphosphatidylinositolkinaseaktrelatedtotheaandckinaseinchildrenwithcholesteatomainmiddleear AT linhan theeffectofzbxz23ir21nanonanomaterialsdeliveryvectoronapoptosisandptenphosphataseandtensinhomologdeletedonchromosometenpi3kintracellularphosphatidylinositolkinaseaktrelatedtotheaandckinaseinchildrenwithcholesteatomainmiddleear AT hongweizheng effectofzbxz23ir21nanonanomaterialsdeliveryvectoronapoptosisandptenphosphataseandtensinhomologdeletedonchromosometenpi3kintracellularphosphatidylinositolkinaseaktrelatedtotheaandckinaseinchildrenwithcholesteatomainmiddleear AT wenlunwang effectofzbxz23ir21nanonanomaterialsdeliveryvectoronapoptosisandptenphosphataseandtensinhomologdeletedonchromosometenpi3kintracellularphosphatidylinositolkinaseaktrelatedtotheaandckinaseinchildrenwithcholesteatomainmiddleear AT shichangli effectofzbxz23ir21nanonanomaterialsdeliveryvectoronapoptosisandptenphosphataseandtensinhomologdeletedonchromosometenpi3kintracellularphosphatidylinositolkinaseaktrelatedtotheaandckinaseinchildrenwithcholesteatomainmiddleear AT linhan effectofzbxz23ir21nanonanomaterialsdeliveryvectoronapoptosisandptenphosphataseandtensinhomologdeletedonchromosometenpi3kintracellularphosphatidylinositolkinaseaktrelatedtotheaandckinaseinchildrenwithcholesteatomainmiddleear
_version_	1718444728191549440

The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear

Ejemplares similares