Linear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA delivery in vitro

Julio C Fernandes,1 Xingping Qiu,2 Françoise M Winnik,2,5 Mohamed Benderdour,1 Xiaoling Zhang,3,4 Kerong Dai,3,4 Qin Shi1 1Orthopaedics Research Laboratory, Research Centre, Sacré-Coeur Hospital, 2Faculty of Pharmacy and Chemistry, University of Montreal, Montreal, Quebec, Cana...

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Autores principales:	Fernandes JC, Qiu X, Winnik FM, Benderdour M, Zhang X, Dai K, Shi Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9189c389735541d190c6fb4c8f6d5f5a
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spelling	oai:doaj.org-article:9189c389735541d190c6fb4c8f6d5f5a2021-12-02T02:17:55ZLinear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA delivery in vitro1176-91141178-2013https://doaj.org/article/9189c389735541d190c6fb4c8f6d5f5a2013-10-01T00:00:00Zhttp://www.dovepress.com/linear-polyethylenimine-produced-by-partial-acid-hydrolysis-of-poly2-e-a14838https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Julio C Fernandes,1 Xingping Qiu,2 Françoise M Winnik,2,5 Mohamed Benderdour,1 Xiaoling Zhang,3,4 Kerong Dai,3,4 Qin Shi1 1Orthopaedics Research Laboratory, Research Centre, Sacré-Coeur Hospital, 2Faculty of Pharmacy and Chemistry, University of Montreal, Montreal, Quebec, Canada; 3Orthopaedic Cellular and Molecular Biology Laboratories, Institute of Health Sciences, Chinese Academy of Sciences, 4Department of Orthopaedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; 5WPI International Center for Materials Nanoarchitectonics, National Institute for Materials Science, Ibaraki, Tsukuba, Japan Abstract: Polyethylenimines (PEIs) are the most efficient synthetic vectors for gene delivery available to date. With its high charge density and strong proton-buffering effect, PEI has an ability to condense DNA and small interfering RNA at physiologic pH. However, the polymer suffers from the disadvantage of high cellular toxicity. To reduce its cellular toxicity, we synthesized linear PEIs by partial hydrolysis of poly(2-ethyl-2-oxazoline). Three linear PEIs with different hydrolysis percentages (30%, 70%, and 96%, respectively) were produced as PEI30, PEI70, and PEI96. PEI30 and PEI96 cannot be considered as suitable transfection agents because of low transfection efficiency (PEI30) or high cellular toxicity (PEI96). PEI70 displayed very weak cell toxicity. The charge density of this polymer (PEI70) was strong enough to condense DNA and small interfering RNA at a physiologic pH of 7.4. Our results also show that PEI70 was highly efficient in DNA delivery and small interfering RNA-mediated knockdown of target genes. Thus, polymers such as PEI70 appear to be very promising vectors for gene delivery. Keywords: nonviral vector, polyethylenimine, gene delivery, DNA, small interfering RNAFernandes JCQiu XWinnik FMBenderdour MZhang XDai KShi QDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 4091-4102 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Fernandes JC Qiu X Winnik FM Benderdour M Zhang X Dai K Shi Q Linear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA delivery in vitro
description	Julio C Fernandes,1 Xingping Qiu,2 Françoise M Winnik,2,5 Mohamed Benderdour,1 Xiaoling Zhang,3,4 Kerong Dai,3,4 Qin Shi1 1Orthopaedics Research Laboratory, Research Centre, Sacré-Coeur Hospital, 2Faculty of Pharmacy and Chemistry, University of Montreal, Montreal, Quebec, Canada; 3Orthopaedic Cellular and Molecular Biology Laboratories, Institute of Health Sciences, Chinese Academy of Sciences, 4Department of Orthopaedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; 5WPI International Center for Materials Nanoarchitectonics, National Institute for Materials Science, Ibaraki, Tsukuba, Japan Abstract: Polyethylenimines (PEIs) are the most efficient synthetic vectors for gene delivery available to date. With its high charge density and strong proton-buffering effect, PEI has an ability to condense DNA and small interfering RNA at physiologic pH. However, the polymer suffers from the disadvantage of high cellular toxicity. To reduce its cellular toxicity, we synthesized linear PEIs by partial hydrolysis of poly(2-ethyl-2-oxazoline). Three linear PEIs with different hydrolysis percentages (30%, 70%, and 96%, respectively) were produced as PEI30, PEI70, and PEI96. PEI30 and PEI96 cannot be considered as suitable transfection agents because of low transfection efficiency (PEI30) or high cellular toxicity (PEI96). PEI70 displayed very weak cell toxicity. The charge density of this polymer (PEI70) was strong enough to condense DNA and small interfering RNA at a physiologic pH of 7.4. Our results also show that PEI70 was highly efficient in DNA delivery and small interfering RNA-mediated knockdown of target genes. Thus, polymers such as PEI70 appear to be very promising vectors for gene delivery. Keywords: nonviral vector, polyethylenimine, gene delivery, DNA, small interfering RNA
format	article
author	Fernandes JC Qiu X Winnik FM Benderdour M Zhang X Dai K Shi Q
author_facet	Fernandes JC Qiu X Winnik FM Benderdour M Zhang X Dai K Shi Q
author_sort	Fernandes JC
title	Linear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA delivery in vitro
title_short	Linear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA delivery in vitro
title_full	Linear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA delivery in vitro
title_fullStr	Linear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA delivery in vitro
title_full_unstemmed	Linear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA delivery in vitro
title_sort	linear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for dna and sirna delivery in vitro
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/9189c389735541d190c6fb4c8f6d5f5a
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Linear polyethylenimine produced by partial acid hydrolysis of poly(2-ethyl-2-oxazoline) for DNA and siRNA delivery in vitro

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