Why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?

Abstract Extraction of DNA and RNA from formalin-fixed paraffin-embedded (FFPE) tissue blocks is a critical process in molecular oncology testing. Using FFPE, it is possible to choose the portion of tissue to study, taking into account the cell morphology, storage stability and storage conditions at...

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Autores principales:	Rafael Parra-Medina, Sandra Ramírez-Clavijo
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Prostate cancer; FFPE Punch Microdissection Molecular Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/918f7b9eff0b4690853d15e2e2fe5dc3
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spelling	oai:doaj.org-article:918f7b9eff0b4690853d15e2e2fe5dc32021-11-28T12:14:30ZWhy not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?10.1186/s12301-021-00257-41110-57041961-9987https://doaj.org/article/918f7b9eff0b4690853d15e2e2fe5dc32021-11-01T00:00:00Zhttps://doi.org/10.1186/s12301-021-00257-4https://doaj.org/toc/1110-5704https://doaj.org/toc/1961-9987Abstract Extraction of DNA and RNA from formalin-fixed paraffin-embedded (FFPE) tissue blocks is a critical process in molecular oncology testing. Using FFPE, it is possible to choose the portion of tissue to study, taking into account the cell morphology, storage stability and storage conditions at room temperature, and make retrospective studies with clinical and pathological information. In prostate cancer tissue, in contrast with macroscopic tumors, it is not easy to identify the tumor; therefore, it is very important to make a microscopic diagnosis. We do not recommend punching this tissue because it can choose normal tissue for molecular analysis. In the present article we review the differences between punch biopsy and microdissection.Rafael Parra-MedinaSandra Ramírez-ClavijoSpringerOpenarticleProstate cancer; FFPEPunchMicrodissectionMolecularDiseases of the genitourinary system. UrologyRC870-923ENAfrican Journal of Urology, Vol 27, Iss 1, Pp 1-5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Prostate cancer; FFPE Punch Microdissection Molecular Diseases of the genitourinary system. Urology RC870-923
spellingShingle	Prostate cancer; FFPE Punch Microdissection Molecular Diseases of the genitourinary system. Urology RC870-923 Rafael Parra-Medina Sandra Ramírez-Clavijo Why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?
description	Abstract Extraction of DNA and RNA from formalin-fixed paraffin-embedded (FFPE) tissue blocks is a critical process in molecular oncology testing. Using FFPE, it is possible to choose the portion of tissue to study, taking into account the cell morphology, storage stability and storage conditions at room temperature, and make retrospective studies with clinical and pathological information. In prostate cancer tissue, in contrast with macroscopic tumors, it is not easy to identify the tumor; therefore, it is very important to make a microscopic diagnosis. We do not recommend punching this tissue because it can choose normal tissue for molecular analysis. In the present article we review the differences between punch biopsy and microdissection.
format	article
author	Rafael Parra-Medina Sandra Ramírez-Clavijo
author_facet	Rafael Parra-Medina Sandra Ramírez-Clavijo
author_sort	Rafael Parra-Medina
title	Why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?
title_short	Why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?
title_full	Why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?
title_fullStr	Why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?
title_full_unstemmed	Why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?
title_sort	why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for dna and rna extraction?
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/918f7b9eff0b4690853d15e2e2fe5dc3
work_keys_str_mv	AT rafaelparramedina whynottousepunchbiopsiesinformalinfixedparaffinembeddedsamplesofprostatecancertissuefordnaandrnaextraction AT sandraramirezclavijo whynottousepunchbiopsiesinformalinfixedparaffinembeddedsamplesofprostatecancertissuefordnaandrnaextraction
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Why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?

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