FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION

Subject: to assess a role of factors of innate and adaptive immunity in the development of intrauterine generalized cytomegalovirus infection.The study included 47 newborns with congenital generalized cytomegalovirus infection comprising group I. Based on the data of clinical and laboratory examinat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	M. A. Levkovich, L. V. Kravchenko, A. A. Afonin, I. I. Krukier, A. Yu. Levkovich, A. A. Nikashina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	innate immunity tlr receptors cytokines intrauterine infection cytomegalovirus newborn children Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9194eb9b6ebe441f9d06efaa304afb39
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9194eb9b6ebe441f9d06efaa304afb39
record_format	dspace
spelling	oai:doaj.org-article:9194eb9b6ebe441f9d06efaa304afb392021-11-18T08:03:51ZFACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION1563-06252313-741X10.15789/1563-0625-FOC-2303https://doaj.org/article/9194eb9b6ebe441f9d06efaa304afb392021-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2420https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XSubject: to assess a role of factors of innate and adaptive immunity in the development of intrauterine generalized cytomegalovirus infection.The study included 47 newborns with congenital generalized cytomegalovirus infection comprising group I. Based on the data of clinical and laboratory examination, all newborns studied were divided into two subgroups. Subgroup 1.1 (29 subjects) consisted of newborns with severe CMVI and subgroup 1.2 (18 subjects) – newborns with moderate CMVI. The control group included 26 newborns without herpesvirus infection.Determination of the number of monocytes expressing Toll receptors (TLR) was performed by laser flow cytometry (Beckman Coulter) using Beckman Coulter, HyCultBiotechnology reagents: FITC-CD282+, CD284+, CD286+, and PE-CD14+. The newborn serum concentration of IFNγ, IFNα, IL-6, IL-8 was determined by ELISA using BenderMedsistems test systems.Intrauterine generalized CMVI with complete clinical symptoms in newborns was characterized by a decrease in the number of monocytes expressing TLR-2 and TLR-6, which was associated with a decrease in the level of IFNα, IFNγ, an increase in the level of IL-6, IL-8 and MCP-1. The subgroup with incomplete clinical symptoms CMVI was characterized by a decrease in the level of IFNα, in combination with an increase in the level of IL-6. The identified immune disorders lead to a reduction in the antiviral immune response and determine the severity of the disease in prenatally infected newborns.M. A. LevkovichL. V. KravchenkoA. A. AfoninI. I. KrukierA. Yu. LevkovichA. A. NikashinaSPb RAACIarticleinnate immunitytlr receptorscytokinesintrauterine infectioncytomegalovirusnewborn childrenImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 4, Pp 799-804 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	innate immunity tlr receptors cytokines intrauterine infection cytomegalovirus newborn children Immunologic diseases. Allergy RC581-607
spellingShingle	innate immunity tlr receptors cytokines intrauterine infection cytomegalovirus newborn children Immunologic diseases. Allergy RC581-607 M. A. Levkovich L. V. Kravchenko A. A. Afonin I. I. Krukier A. Yu. Levkovich A. A. Nikashina FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION
description	Subject: to assess a role of factors of innate and adaptive immunity in the development of intrauterine generalized cytomegalovirus infection.The study included 47 newborns with congenital generalized cytomegalovirus infection comprising group I. Based on the data of clinical and laboratory examination, all newborns studied were divided into two subgroups. Subgroup 1.1 (29 subjects) consisted of newborns with severe CMVI and subgroup 1.2 (18 subjects) – newborns with moderate CMVI. The control group included 26 newborns without herpesvirus infection.Determination of the number of monocytes expressing Toll receptors (TLR) was performed by laser flow cytometry (Beckman Coulter) using Beckman Coulter, HyCultBiotechnology reagents: FITC-CD282+, CD284+, CD286+, and PE-CD14+. The newborn serum concentration of IFNγ, IFNα, IL-6, IL-8 was determined by ELISA using BenderMedsistems test systems.Intrauterine generalized CMVI with complete clinical symptoms in newborns was characterized by a decrease in the number of monocytes expressing TLR-2 and TLR-6, which was associated with a decrease in the level of IFNα, IFNγ, an increase in the level of IL-6, IL-8 and MCP-1. The subgroup with incomplete clinical symptoms CMVI was characterized by a decrease in the level of IFNα, in combination with an increase in the level of IL-6. The identified immune disorders lead to a reduction in the antiviral immune response and determine the severity of the disease in prenatally infected newborns.
format	article
author	M. A. Levkovich L. V. Kravchenko A. A. Afonin I. I. Krukier A. Yu. Levkovich A. A. Nikashina
author_facet	M. A. Levkovich L. V. Kravchenko A. A. Afonin I. I. Krukier A. Yu. Levkovich A. A. Nikashina
author_sort	M. A. Levkovich
title	FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION
title_short	FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION
title_full	FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION
title_fullStr	FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION
title_full_unstemmed	FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION
title_sort	factors of congenital and adaptive immunity in the pathogenesis of intrauterine generated cytomegalovirus infection
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/9194eb9b6ebe441f9d06efaa304afb39
work_keys_str_mv	AT malevkovich factorsofcongenitalandadaptiveimmunityinthepathogenesisofintrauterinegeneratedcytomegalovirusinfection AT lvkravchenko factorsofcongenitalandadaptiveimmunityinthepathogenesisofintrauterinegeneratedcytomegalovirusinfection AT aaafonin factorsofcongenitalandadaptiveimmunityinthepathogenesisofintrauterinegeneratedcytomegalovirusinfection AT iikrukier factorsofcongenitalandadaptiveimmunityinthepathogenesisofintrauterinegeneratedcytomegalovirusinfection AT ayulevkovich factorsofcongenitalandadaptiveimmunityinthepathogenesisofintrauterinegeneratedcytomegalovirusinfection AT aanikashina factorsofcongenitalandadaptiveimmunityinthepathogenesisofintrauterinegeneratedcytomegalovirusinfection
_version_	1718422300341043200

FACTORS OF CONGENITAL AND ADAPTIVE IMMUNITY IN THE PATHOGENESIS OF INTRAUTERINE GENERATED CYTOMEGALOVIRUS INFECTION

Ejemplares similares