Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls

Abstract Tape stripping is a promising technique for assessment of epidermal biomarkers in inflammatory skin diseases. However, to facilitate its implementation in the clinical practice, a thorough validation regarding sampling strategy is needed. Knowledge of biomarkers variation in concentration a...

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Autores principales:	Maja-Lisa Clausen, S. Kezic, C. M. Olesen, T. Agner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9199900d75a042b681ce56cad6f8d5f5
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spelling	oai:doaj.org-article:9199900d75a042b681ce56cad6f8d5f52021-12-02T13:58:11ZCytokine concentration across the stratum corneum in atopic dermatitis and healthy controls10.1038/s41598-020-78943-62045-2322https://doaj.org/article/9199900d75a042b681ce56cad6f8d5f52020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78943-6https://doaj.org/toc/2045-2322Abstract Tape stripping is a promising technique for assessment of epidermal biomarkers in inflammatory skin diseases. However, to facilitate its implementation in the clinical practice, a thorough validation regarding sampling strategy is needed. Knowledge of biomarkers variation in concentration across stratum corneum is scarce. Therefore, this study aimed to assess the variability of cytokines across stratum corneum using tape stripping technique by consecutive application of 21 adhesive tapes (D-squame) to lesional and non-lesional skin from 15 patients with atopic dermatitis (AD) and 16 healthy controls. Concentration of cytokines (IL-1α, IL-1b, IL-5, IL-18, IFN-γ, CCL17, CCL22, CCL27, CXCL8, CXCL10, TNF-α, TSLP, VEGFA) was determined in five different depths, using multiplex immunoassay. Comparing tape 4 with tape 21, no cytokine changed significantly in concentration in AD lesional skin. In AD non-lesional skin a small decrease was found for CCL17, CXCL8 and CXCL10. For healthy controls, a decrease was found for IL-1a, IL-1b, VEGFA and an increase for IL-18. Differences were found between AD skin and healthy control skin. Concentration of cytokines was stable across stratum corneum, indicating that sampling of only one tape from the stratum corneum is reliable in reflecting the overall cytokine milieu. Differences between AD and healthy skin confirm robustness of tape stripping for measuring cytokine levels.Maja-Lisa ClausenS. KezicC. M. OlesenT. AgnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maja-Lisa Clausen S. Kezic C. M. Olesen T. Agner Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls
description	Abstract Tape stripping is a promising technique for assessment of epidermal biomarkers in inflammatory skin diseases. However, to facilitate its implementation in the clinical practice, a thorough validation regarding sampling strategy is needed. Knowledge of biomarkers variation in concentration across stratum corneum is scarce. Therefore, this study aimed to assess the variability of cytokines across stratum corneum using tape stripping technique by consecutive application of 21 adhesive tapes (D-squame) to lesional and non-lesional skin from 15 patients with atopic dermatitis (AD) and 16 healthy controls. Concentration of cytokines (IL-1α, IL-1b, IL-5, IL-18, IFN-γ, CCL17, CCL22, CCL27, CXCL8, CXCL10, TNF-α, TSLP, VEGFA) was determined in five different depths, using multiplex immunoassay. Comparing tape 4 with tape 21, no cytokine changed significantly in concentration in AD lesional skin. In AD non-lesional skin a small decrease was found for CCL17, CXCL8 and CXCL10. For healthy controls, a decrease was found for IL-1a, IL-1b, VEGFA and an increase for IL-18. Differences were found between AD skin and healthy control skin. Concentration of cytokines was stable across stratum corneum, indicating that sampling of only one tape from the stratum corneum is reliable in reflecting the overall cytokine milieu. Differences between AD and healthy skin confirm robustness of tape stripping for measuring cytokine levels.
format	article
author	Maja-Lisa Clausen S. Kezic C. M. Olesen T. Agner
author_facet	Maja-Lisa Clausen S. Kezic C. M. Olesen T. Agner
author_sort	Maja-Lisa Clausen
title	Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls
title_short	Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls
title_full	Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls
title_fullStr	Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls
title_full_unstemmed	Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls
title_sort	cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9199900d75a042b681ce56cad6f8d5f5
work_keys_str_mv	AT majalisaclausen cytokineconcentrationacrossthestratumcorneuminatopicdermatitisandhealthycontrols AT skezic cytokineconcentrationacrossthestratumcorneuminatopicdermatitisandhealthycontrols AT cmolesen cytokineconcentrationacrossthestratumcorneuminatopicdermatitisandhealthycontrols AT tagner cytokineconcentrationacrossthestratumcorneuminatopicdermatitisandhealthycontrols
_version_	1718392212983644160

Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls

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