Identification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping

Abstract Genetic resistance is a successful strategy for management of clubroot (Plasmodiophora brassicae) of brassica crops, but resistance can break down quickly. Identification of novel sources of resistance is especially important when new pathotypes arise. In the current study, the reaction of...

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Autores principales:	Abdulsalam Dakouri, Mebarek Lamara, Md. Masud Karim, Jinghe Wang, Qilin Chen, Bruce D. Gossen, Stephen E. Strelkov, Sheau-Fang Hwang, Gary Peng, Fengqun Yu
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Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/919a8073e3d1466ebf0d229c260706cb
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spelling	oai:doaj.org-article:919a8073e3d1466ebf0d229c260706cb2021-12-02T11:45:01ZIdentification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping10.1038/s41598-021-85836-92045-2322https://doaj.org/article/919a8073e3d1466ebf0d229c260706cb2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85836-9https://doaj.org/toc/2045-2322Abstract Genetic resistance is a successful strategy for management of clubroot (Plasmodiophora brassicae) of brassica crops, but resistance can break down quickly. Identification of novel sources of resistance is especially important when new pathotypes arise. In the current study, the reaction of 177 accessions of Brassica napus to four new, virulent pathotypes of P. brassicae was assessed. Each accession was genotyped using genotyping by sequencing to identify and map novel sources of clubroot resistance using mixed linear model (MLM) analysis. The majority of accessions were highly susceptible (70–100 DSI), but a few accessions exhibited strong resistance (0–20 DSI) to pathotypes 5X (21 accessions), 3A (8), 2B (7), and 3D (15), based on the Canadian Clubroot Differential system. In total, 301,753 SNPs were mapped to 19 chromosomes. Population structure analysis indicated that the 177 accessions belong to seven major populations. SNPs were associated with resistance to each pathotype using MLM. In total, 13 important SNP loci were identified, with 9 SNPs mapped to the A-genome and 4 to the C-genome. The SNPs were associated with resistance to pathotypes 5X (2 SNPs), 3A (4), 2B (5) and 3D (6). A Blast search of 1.6 Mb upstream and downstream from each SNP identified 13 disease-resistance genes or domains. The distance between a SNP locus and the nearest resistance gene ranged from 0.04 to 0.74 Mb. The resistant lines and SNP markers identified in this study can be used to breed for resistance to the most prevalent new pathotypes of P. brassicae in Canada.Abdulsalam DakouriMebarek LamaraMd. Masud KarimJinghe WangQilin ChenBruce D. GossenStephen E. StrelkovSheau-Fang HwangGary PengFengqun YuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abdulsalam Dakouri Mebarek Lamara Md. Masud Karim Jinghe Wang Qilin Chen Bruce D. Gossen Stephen E. Strelkov Sheau-Fang Hwang Gary Peng Fengqun Yu Identification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping
description	Abstract Genetic resistance is a successful strategy for management of clubroot (Plasmodiophora brassicae) of brassica crops, but resistance can break down quickly. Identification of novel sources of resistance is especially important when new pathotypes arise. In the current study, the reaction of 177 accessions of Brassica napus to four new, virulent pathotypes of P. brassicae was assessed. Each accession was genotyped using genotyping by sequencing to identify and map novel sources of clubroot resistance using mixed linear model (MLM) analysis. The majority of accessions were highly susceptible (70–100 DSI), but a few accessions exhibited strong resistance (0–20 DSI) to pathotypes 5X (21 accessions), 3A (8), 2B (7), and 3D (15), based on the Canadian Clubroot Differential system. In total, 301,753 SNPs were mapped to 19 chromosomes. Population structure analysis indicated that the 177 accessions belong to seven major populations. SNPs were associated with resistance to each pathotype using MLM. In total, 13 important SNP loci were identified, with 9 SNPs mapped to the A-genome and 4 to the C-genome. The SNPs were associated with resistance to pathotypes 5X (2 SNPs), 3A (4), 2B (5) and 3D (6). A Blast search of 1.6 Mb upstream and downstream from each SNP identified 13 disease-resistance genes or domains. The distance between a SNP locus and the nearest resistance gene ranged from 0.04 to 0.74 Mb. The resistant lines and SNP markers identified in this study can be used to breed for resistance to the most prevalent new pathotypes of P. brassicae in Canada.
format	article
author	Abdulsalam Dakouri Mebarek Lamara Md. Masud Karim Jinghe Wang Qilin Chen Bruce D. Gossen Stephen E. Strelkov Sheau-Fang Hwang Gary Peng Fengqun Yu
author_facet	Abdulsalam Dakouri Mebarek Lamara Md. Masud Karim Jinghe Wang Qilin Chen Bruce D. Gossen Stephen E. Strelkov Sheau-Fang Hwang Gary Peng Fengqun Yu
author_sort	Abdulsalam Dakouri
title	Identification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping
title_short	Identification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping
title_full	Identification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping
title_fullStr	Identification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping
title_full_unstemmed	Identification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping
title_sort	identification of resistance loci against new pathotypes of plasmodiophora brassicae in brassica napus based on genome-wide association mapping
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/919a8073e3d1466ebf0d229c260706cb
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Identification of resistance loci against new pathotypes of Plasmodiophora brassicae in Brassica napus based on genome-wide association mapping

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