Gene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots

Yanjie Yang, Peisen Li, Yao Lin, Ziqi Li, Tianyi Cui, Zhenhua Song, Weixia Wu, Shuangyu Lv, Shaoping Ji Henan Provincial Engineering Centre for Tumor Molecular Medicine, Institute of Molecular Medicine, School of Basic Medical Sciences, Henan University, Kaifeng 475004, People’s Republic o...

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Autores principales:	Yang Y, Li P, Lin Y, Li Z, Cui T, Song Z, Wu W, Lv S, Ji S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	zno qds transcriptome differentially expressed genes Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/919e2ffac0ec44e699bb67009dba801a
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spelling	oai:doaj.org-article:919e2ffac0ec44e699bb67009dba801a2021-12-02T09:46:00ZGene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots1178-2013https://doaj.org/article/919e2ffac0ec44e699bb67009dba801a2020-04-01T00:00:00Zhttps://www.dovepress.com/gene-expression-profiling-of-the-liver-and-lung-in-mice-after-exposure-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yanjie Yang, Peisen Li, Yao Lin, Ziqi Li, Tianyi Cui, Zhenhua Song, Weixia Wu, Shuangyu Lv, Shaoping Ji Henan Provincial Engineering Centre for Tumor Molecular Medicine, Institute of Molecular Medicine, School of Basic Medical Sciences, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Shuangyu Lv; Shaoping JiSchool of Basic Medical Sciences, Henan University, Kaifeng 475004, People’s Republic of ChinaTel/Fax +86 371 2388 0585Email shuangyulv@henu.edu.cn; shaopingji@henu.edu.cnIntroduction: ZnO quantum dots (QDs) have drawn much attention recently as they are Cd-free, low-cost, and have excellent optical properties. With the expanded production and application of ZnO nanoparticles, concerns about their potential toxicity have also been raised.Materials and Methods: We used RNA sequencing (RNA-seq) to analyze the global gene expression of liver and lung tissues after ZnO QDs treatment. Differentially expressed genes (DEGs) were screened, with a fold change > 1.5 and padj < 0.05. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed, and padj < 0.05 was considered significantly enriched. The RNA-seq results were validated by quantitative real-time polymerase chain reaction (qRT-PCR).Results: A total of 47 and 218 genes were significantly differentially expressed in the liver and lung. Eight GO terms were enriched in the liver and lung, and retinol metabolism and the peroxisome proliferator-activated receptor (PPAR) signaling pathway were shared in different tissues.Discussion: According to DEGs and pathway enrichment analyses, inflammation might be induced in liver and lung tissues after intravenous injection of ZnO QDs. These findings will be helpful for future research and application of ZnO QDs.Keywords: ZnO QDs, transcriptome, differentially expressed genesYang YLi PLin YLi ZCui TSong ZWu WLv SJi SDove Medical Pressarticlezno qdstranscriptomedifferentially expressed genesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2947-2955 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	zno qds transcriptome differentially expressed genes Medicine (General) R5-920
spellingShingle	zno qds transcriptome differentially expressed genes Medicine (General) R5-920 Yang Y Li P Lin Y Li Z Cui T Song Z Wu W Lv S Ji S Gene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots
description	Yanjie Yang, Peisen Li, Yao Lin, Ziqi Li, Tianyi Cui, Zhenhua Song, Weixia Wu, Shuangyu Lv, Shaoping Ji Henan Provincial Engineering Centre for Tumor Molecular Medicine, Institute of Molecular Medicine, School of Basic Medical Sciences, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Shuangyu Lv; Shaoping JiSchool of Basic Medical Sciences, Henan University, Kaifeng 475004, People’s Republic of ChinaTel/Fax +86 371 2388 0585Email shuangyulv@henu.edu.cn; shaopingji@henu.edu.cnIntroduction: ZnO quantum dots (QDs) have drawn much attention recently as they are Cd-free, low-cost, and have excellent optical properties. With the expanded production and application of ZnO nanoparticles, concerns about their potential toxicity have also been raised.Materials and Methods: We used RNA sequencing (RNA-seq) to analyze the global gene expression of liver and lung tissues after ZnO QDs treatment. Differentially expressed genes (DEGs) were screened, with a fold change > 1.5 and padj < 0.05. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed, and padj < 0.05 was considered significantly enriched. The RNA-seq results were validated by quantitative real-time polymerase chain reaction (qRT-PCR).Results: A total of 47 and 218 genes were significantly differentially expressed in the liver and lung. Eight GO terms were enriched in the liver and lung, and retinol metabolism and the peroxisome proliferator-activated receptor (PPAR) signaling pathway were shared in different tissues.Discussion: According to DEGs and pathway enrichment analyses, inflammation might be induced in liver and lung tissues after intravenous injection of ZnO QDs. These findings will be helpful for future research and application of ZnO QDs.Keywords: ZnO QDs, transcriptome, differentially expressed genes
format	article
author	Yang Y Li P Lin Y Li Z Cui T Song Z Wu W Lv S Ji S
author_facet	Yang Y Li P Lin Y Li Z Cui T Song Z Wu W Lv S Ji S
author_sort	Yang Y
title	Gene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots
title_short	Gene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots
title_full	Gene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots
title_fullStr	Gene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots
title_full_unstemmed	Gene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots
title_sort	gene expression profiling of the liver and lung in mice after exposure to zno quantum dots
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/919e2ffac0ec44e699bb67009dba801a
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Gene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots

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