Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1

Abstract Shigellosis, caused by Shigella bacterial spp., is one of the leading causes of diarrheal morbidity and mortality. An increasing prevalence of multidrug-resistant Shigella species has revived the importance of bacteriophages as an alternative therapy to antibiotics. In this study, a novel b...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bani Mallick, Payel Mondal, Moumita Dutta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/919eb97f6f4d4d29bccfe90efb952910
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:919eb97f6f4d4d29bccfe90efb952910
record_format	dspace
spelling	oai:doaj.org-article:919eb97f6f4d4d29bccfe90efb9529102021-12-02T17:37:24ZMorphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae110.1038/s41598-021-98910-z2045-2322https://doaj.org/article/919eb97f6f4d4d29bccfe90efb9529102021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98910-zhttps://doaj.org/toc/2045-2322Abstract Shigellosis, caused by Shigella bacterial spp., is one of the leading causes of diarrheal morbidity and mortality. An increasing prevalence of multidrug-resistant Shigella species has revived the importance of bacteriophages as an alternative therapy to antibiotics. In this study, a novel bacteriophage, Sfk20, has been isolated from water bodies of a diarrheal outbreak area in Kolkata (India) with lytic activity against many Shigella spp. Phage Sfk20 showed a latent period of 20 min and a large burst size of 123 pfu per infected cell in a one-step growth analysis. Phage-host interaction and lytic activity confirmed by phage attachment, intracellular phage development, and bacterial cell burst using ultrathin sectioning and TEM analysis. The genomic analysis revealed that the double-stranded DNA genome of Sfk20 contains 164,878 bp with 35.62% G + C content and 241 ORFs. Results suggested phage Sfk20 to include as a member of the T4 myoviridae bacteriophage group. Phage Sfk20 has shown anti-biofilm potential against Shigella species. The results of this study imply that Sfk20 has good possibilities to be used as a biocontrol agent.Bani MallickPayel MondalMoumita DuttaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bani Mallick Payel Mondal Moumita Dutta Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1
description	Abstract Shigellosis, caused by Shigella bacterial spp., is one of the leading causes of diarrheal morbidity and mortality. An increasing prevalence of multidrug-resistant Shigella species has revived the importance of bacteriophages as an alternative therapy to antibiotics. In this study, a novel bacteriophage, Sfk20, has been isolated from water bodies of a diarrheal outbreak area in Kolkata (India) with lytic activity against many Shigella spp. Phage Sfk20 showed a latent period of 20 min and a large burst size of 123 pfu per infected cell in a one-step growth analysis. Phage-host interaction and lytic activity confirmed by phage attachment, intracellular phage development, and bacterial cell burst using ultrathin sectioning and TEM analysis. The genomic analysis revealed that the double-stranded DNA genome of Sfk20 contains 164,878 bp with 35.62% G + C content and 241 ORFs. Results suggested phage Sfk20 to include as a member of the T4 myoviridae bacteriophage group. Phage Sfk20 has shown anti-biofilm potential against Shigella species. The results of this study imply that Sfk20 has good possibilities to be used as a biocontrol agent.
format	article
author	Bani Mallick Payel Mondal Moumita Dutta
author_facet	Bani Mallick Payel Mondal Moumita Dutta
author_sort	Bani Mallick
title	Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1
title_short	Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1
title_full	Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1
title_fullStr	Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1
title_full_unstemmed	Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1
title_sort	morphological, biological, and genomic characterization of a newly isolated lytic phage sfk20 infecting shigella flexneri, shigella sonnei, and shigella dysenteriae1
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/919eb97f6f4d4d29bccfe90efb952910
work_keys_str_mv	AT banimallick morphologicalbiologicalandgenomiccharacterizationofanewlyisolatedlyticphagesfk20infectingshigellaflexnerishigellasonneiandshigelladysenteriae1 AT payelmondal morphologicalbiologicalandgenomiccharacterizationofanewlyisolatedlyticphagesfk20infectingshigellaflexnerishigellasonneiandshigelladysenteriae1 AT moumitadutta morphologicalbiologicalandgenomiccharacterizationofanewlyisolatedlyticphagesfk20infectingshigellaflexnerishigellasonneiandshigelladysenteriae1
_version_	1718379895827988480

Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1

Ejemplares similares