Immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity

Abstract Using a ground-based model to simulate spaceflight [21-days of single-housed, hindlimb unloading (HLU) combined with continuous low-dose gamma irradiation (LDR, total dose of 0.04 Gy)], an in-depth survey of the immune and hematological systems of mice at 7-days post-exposure was performed....

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Autores principales:	Amber M. Paul, Eliah G. Overbey, Willian A. da Silveira, Nathaniel Szewczyk, Nina C. Nishiyama, Michael J. Pecaut, Sulekha Anand, Jonathan M. Galazka, Xiao Wen Mao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/91a26c1151db4a87aec85ba385b2cd84
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spelling	oai:doaj.org-article:91a26c1151db4a87aec85ba385b2cd842021-12-02T18:25:03ZImmunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity10.1038/s41598-021-90439-52045-2322https://doaj.org/article/91a26c1151db4a87aec85ba385b2cd842021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90439-5https://doaj.org/toc/2045-2322Abstract Using a ground-based model to simulate spaceflight [21-days of single-housed, hindlimb unloading (HLU) combined with continuous low-dose gamma irradiation (LDR, total dose of 0.04 Gy)], an in-depth survey of the immune and hematological systems of mice at 7-days post-exposure was performed. Collected blood was profiled with a hematology analyzer and spleens were analyzed by whole transcriptome shotgun sequencing (RNA-sequencing). The results revealed negligible differences in immune differentials. However, hematological system analyses of whole blood indicated large disparities in red blood cell differentials and morphology, suggestive of anemia. Murine Reactome networks indicated majority of spleen cells displayed differentially expressed genes (DEG) involved in signal transduction, metabolism, cell cycle, chromatin organization, and DNA repair. Although immune differentials were not changed, DEG analysis of the spleen revealed expression profiles associated with inflammation and dysregulated immune function persist to 1-week post-simulated spaceflight. Additionally, specific regulation pathways associated with human blood disease gene orthologs, such as blood pressure regulation, transforming growth factor-β receptor signaling, and B cell differentiation were noted. Collectively, this study revealed differential immune and hematological outcomes 1-week post-simulated spaceflight conditions, suggesting recovery from spaceflight is an unremitting process.Amber M. PaulEliah G. OverbeyWillian A. da SilveiraNathaniel SzewczykNina C. NishiyamaMichael J. PecautSulekha AnandJonathan M. GalazkaXiao Wen MaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amber M. Paul Eliah G. Overbey Willian A. da Silveira Nathaniel Szewczyk Nina C. Nishiyama Michael J. Pecaut Sulekha Anand Jonathan M. Galazka Xiao Wen Mao Immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity
description	Abstract Using a ground-based model to simulate spaceflight [21-days of single-housed, hindlimb unloading (HLU) combined with continuous low-dose gamma irradiation (LDR, total dose of 0.04 Gy)], an in-depth survey of the immune and hematological systems of mice at 7-days post-exposure was performed. Collected blood was profiled with a hematology analyzer and spleens were analyzed by whole transcriptome shotgun sequencing (RNA-sequencing). The results revealed negligible differences in immune differentials. However, hematological system analyses of whole blood indicated large disparities in red blood cell differentials and morphology, suggestive of anemia. Murine Reactome networks indicated majority of spleen cells displayed differentially expressed genes (DEG) involved in signal transduction, metabolism, cell cycle, chromatin organization, and DNA repair. Although immune differentials were not changed, DEG analysis of the spleen revealed expression profiles associated with inflammation and dysregulated immune function persist to 1-week post-simulated spaceflight. Additionally, specific regulation pathways associated with human blood disease gene orthologs, such as blood pressure regulation, transforming growth factor-β receptor signaling, and B cell differentiation were noted. Collectively, this study revealed differential immune and hematological outcomes 1-week post-simulated spaceflight conditions, suggesting recovery from spaceflight is an unremitting process.
format	article
author	Amber M. Paul Eliah G. Overbey Willian A. da Silveira Nathaniel Szewczyk Nina C. Nishiyama Michael J. Pecaut Sulekha Anand Jonathan M. Galazka Xiao Wen Mao
author_facet	Amber M. Paul Eliah G. Overbey Willian A. da Silveira Nathaniel Szewczyk Nina C. Nishiyama Michael J. Pecaut Sulekha Anand Jonathan M. Galazka Xiao Wen Mao
author_sort	Amber M. Paul
title	Immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity
title_short	Immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity
title_full	Immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity
title_fullStr	Immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity
title_full_unstemmed	Immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity
title_sort	immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/91a26c1151db4a87aec85ba385b2cd84
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Immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity

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