Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing

The continuous discharge of toxic materials into the environment has been an alarming issue faced around the globe. Hence, matching the effort of monitoring activity is vital to coping with the overwhelming amount of metal ions. Along with the significant current research being conducted, this study...

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Autores principales:	Noreen Nordin, Rahmath Abdulla, Siti Aqlima Ahmad, Mohd Khalizan Sabullah
Formato:	article
Lenguaje:	EN
Publicado:	Tamkang University Press 2021
Materias:	acetylcholinesterase diodon hystrix heavy metals pollution Engineering (General). Civil engineering (General) TA1-2040 Chemical engineering TP155-156 Physics QC1-999
Acceso en línea:	https://doaj.org/article/91ae6d2985ff40b08be944e480bd9502
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spelling	oai:doaj.org-article:91ae6d2985ff40b08be944e480bd95022021-11-27T10:26:48ZAcetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing10.6180/jase.202206_25(3).00142708-99672708-9975https://doaj.org/article/91ae6d2985ff40b08be944e480bd95022021-11-01T00:00:00Zhttp://jase.tku.edu.tw/articles/jase-202206-25-3-0014https://doaj.org/toc/2708-9967https://doaj.org/toc/2708-9975The continuous discharge of toxic materials into the environment has been an alarming issue faced around the globe. Hence, matching the effort of monitoring activity is vital to coping with the overwhelming amount of metal ions. Along with the significant current research being conducted, this study aims to investigate the sensitivity of acetylcholinesterase (AChE) of Sabah porcupine fish, Diodon hystrix as an alternative biosensor in the detection of heavy metals. The enzyme was precipitated followed by the purification using ammonium sulfate precipitation and procainamide-affinity chromatography, respectively, with a total recovery of 66.67% with the specific activity of 2297.50 U/mg. The enzyme works optimally at pH 9 with the best incubation temperature of 30°C. The Michaelis constant (Km) and maximal velocity (Vmax) of 1.171 mM and 879257 mol/min/mg denotes the highest catalytic efficiency (Vmax/Km) of acetylthiocholine iodide (ATC) as its preferable substrate. Inhibition study tested on 10 metal ions resulted in increasing toxicity order of Cr6+ < Co2+ < Ag2+ < Cu2+ < Pb2+ < As5+ < Cd2+ < Zn2+ < Ni2+ < Hg2+, with only Hg2+ exhibited the half-maximal inhibitory concentration (IC50) of 0.48 mg/L. From the study, it suggests that the D. hystrix AChE as the potential conventional biosensor for heavy metals detection.Noreen NordinRahmath AbdullaSiti Aqlima AhmadMohd Khalizan SabullahTamkang University Pressarticleacetylcholinesterasediodon hystrixheavy metalspollutionEngineering (General). Civil engineering (General)TA1-2040Chemical engineeringTP155-156PhysicsQC1-999ENJournal of Applied Science and Engineering, Vol 25, Iss 3, Pp 473-480 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acetylcholinesterase diodon hystrix heavy metals pollution Engineering (General). Civil engineering (General) TA1-2040 Chemical engineering TP155-156 Physics QC1-999
spellingShingle	acetylcholinesterase diodon hystrix heavy metals pollution Engineering (General). Civil engineering (General) TA1-2040 Chemical engineering TP155-156 Physics QC1-999 Noreen Nordin Rahmath Abdulla Siti Aqlima Ahmad Mohd Khalizan Sabullah Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing
description	The continuous discharge of toxic materials into the environment has been an alarming issue faced around the globe. Hence, matching the effort of monitoring activity is vital to coping with the overwhelming amount of metal ions. Along with the significant current research being conducted, this study aims to investigate the sensitivity of acetylcholinesterase (AChE) of Sabah porcupine fish, Diodon hystrix as an alternative biosensor in the detection of heavy metals. The enzyme was precipitated followed by the purification using ammonium sulfate precipitation and procainamide-affinity chromatography, respectively, with a total recovery of 66.67% with the specific activity of 2297.50 U/mg. The enzyme works optimally at pH 9 with the best incubation temperature of 30°C. The Michaelis constant (Km) and maximal velocity (Vmax) of 1.171 mM and 879257 mol/min/mg denotes the highest catalytic efficiency (Vmax/Km) of acetylthiocholine iodide (ATC) as its preferable substrate. Inhibition study tested on 10 metal ions resulted in increasing toxicity order of Cr6+ < Co2+ < Ag2+ < Cu2+ < Pb2+ < As5+ < Cd2+ < Zn2+ < Ni2+ < Hg2+, with only Hg2+ exhibited the half-maximal inhibitory concentration (IC50) of 0.48 mg/L. From the study, it suggests that the D. hystrix AChE as the potential conventional biosensor for heavy metals detection.
format	article
author	Noreen Nordin Rahmath Abdulla Siti Aqlima Ahmad Mohd Khalizan Sabullah
author_facet	Noreen Nordin Rahmath Abdulla Siti Aqlima Ahmad Mohd Khalizan Sabullah
author_sort	Noreen Nordin
title	Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing
title_short	Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing
title_full	Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing
title_fullStr	Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing
title_full_unstemmed	Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing
title_sort	acetylcholinesterase (ache) of diodon hystrix brain as an alternative biomolecule in heavy metals biosensing
publisher	Tamkang University Press
publishDate	2021
url	https://doaj.org/article/91ae6d2985ff40b08be944e480bd9502
work_keys_str_mv	AT noreennordin acetylcholinesteraseacheofdiodonhystrixbrainasanalternativebiomoleculeinheavymetalsbiosensing AT rahmathabdulla acetylcholinesteraseacheofdiodonhystrixbrainasanalternativebiomoleculeinheavymetalsbiosensing AT sitiaqlimaahmad acetylcholinesteraseacheofdiodonhystrixbrainasanalternativebiomoleculeinheavymetalsbiosensing AT mohdkhalizansabullah acetylcholinesteraseacheofdiodonhystrixbrainasanalternativebiomoleculeinheavymetalsbiosensing
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Acetylcholinesterase (AChE) of Diodon hystrix brain as an alternative biomolecule in heavy metals biosensing

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