Furry is required for cell movements during gastrulation and functionally interacts with NDR1

Abstract Gastrulation is a key event in animal embryogenesis during which germ layer precursors are rearranged and the embryonic axes are established. Cell polarization is essential during gastrulation, driving asymmetric cell division, cell movements, and cell shape changes. The furry (fry) gene en...

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Autores principales:	Ailen S. Cervino, Bruno Moretti, Carsten Stuckenholz, Hernán E. Grecco, Lance A. Davidson, M. Cecilia Cirio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/91b0460199284f21af1acc202b4c1e9e
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spelling	oai:doaj.org-article:91b0460199284f21af1acc202b4c1e9e2021-12-02T16:36:04ZFurry is required for cell movements during gastrulation and functionally interacts with NDR110.1038/s41598-021-86153-x2045-2322https://doaj.org/article/91b0460199284f21af1acc202b4c1e9e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86153-xhttps://doaj.org/toc/2045-2322Abstract Gastrulation is a key event in animal embryogenesis during which germ layer precursors are rearranged and the embryonic axes are established. Cell polarization is essential during gastrulation, driving asymmetric cell division, cell movements, and cell shape changes. The furry (fry) gene encodes an evolutionarily conserved protein with a wide variety of cellular functions, including cell polarization and morphogenesis in invertebrates. However, little is known about its function in vertebrate development. Here, we show that in Xenopus, Fry plays a role in morphogenetic processes during gastrulation, in addition to its previously described function in the regulation of dorsal mesoderm gene expression. Using morpholino knock-down, we demonstrate a distinct role for Fry in blastopore closure and dorsal axis elongation. Loss of Fry function drastically affects the movement and morphological polarization of cells during gastrulation and disrupts dorsal mesoderm convergent extension, responsible for head-to-tail elongation. Finally, we evaluate a functional interaction between Fry and NDR1 kinase, providing evidence of an evolutionarily conserved complex required for morphogenesis.Ailen S. CervinoBruno MorettiCarsten StuckenholzHernán E. GreccoLance A. DavidsonM. Cecilia CirioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ailen S. Cervino Bruno Moretti Carsten Stuckenholz Hernán E. Grecco Lance A. Davidson M. Cecilia Cirio Furry is required for cell movements during gastrulation and functionally interacts with NDR1
description	Abstract Gastrulation is a key event in animal embryogenesis during which germ layer precursors are rearranged and the embryonic axes are established. Cell polarization is essential during gastrulation, driving asymmetric cell division, cell movements, and cell shape changes. The furry (fry) gene encodes an evolutionarily conserved protein with a wide variety of cellular functions, including cell polarization and morphogenesis in invertebrates. However, little is known about its function in vertebrate development. Here, we show that in Xenopus, Fry plays a role in morphogenetic processes during gastrulation, in addition to its previously described function in the regulation of dorsal mesoderm gene expression. Using morpholino knock-down, we demonstrate a distinct role for Fry in blastopore closure and dorsal axis elongation. Loss of Fry function drastically affects the movement and morphological polarization of cells during gastrulation and disrupts dorsal mesoderm convergent extension, responsible for head-to-tail elongation. Finally, we evaluate a functional interaction between Fry and NDR1 kinase, providing evidence of an evolutionarily conserved complex required for morphogenesis.
format	article
author	Ailen S. Cervino Bruno Moretti Carsten Stuckenholz Hernán E. Grecco Lance A. Davidson M. Cecilia Cirio
author_facet	Ailen S. Cervino Bruno Moretti Carsten Stuckenholz Hernán E. Grecco Lance A. Davidson M. Cecilia Cirio
author_sort	Ailen S. Cervino
title	Furry is required for cell movements during gastrulation and functionally interacts with NDR1
title_short	Furry is required for cell movements during gastrulation and functionally interacts with NDR1
title_full	Furry is required for cell movements during gastrulation and functionally interacts with NDR1
title_fullStr	Furry is required for cell movements during gastrulation and functionally interacts with NDR1
title_full_unstemmed	Furry is required for cell movements during gastrulation and functionally interacts with NDR1
title_sort	furry is required for cell movements during gastrulation and functionally interacts with ndr1
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/91b0460199284f21af1acc202b4c1e9e
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Furry is required for cell movements during gastrulation and functionally interacts with NDR1

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