Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

AC Popa,1,2 GE Stan,1 MA Husanu,1 I Mercioniu,1 LF Santos,3 HR Fernandes,4 JMF Ferreira4 1National Institute of Materials Physics, Măgurele, 2Army Centre for Medical Research, Bucharest, Romania; 3Centro de Química Estrutural, Instituto Superior Técnico (CQE-IST), University o...

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Autores principales:	Popa AC, Stan GE, Husanu MA, Mercioniu I, Santos LF, Fernandes HR, Ferreira JMF
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	biomaterials bioglass in vitro biomimetic assays proteins. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/91b8bb6f6be845f3801c4859840705a2
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spelling	oai:doaj.org-article:91b8bb6f6be845f3801c4859840705a22021-12-02T02:21:11ZBioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry1178-2013https://doaj.org/article/91b8bb6f6be845f3801c4859840705a22017-01-01T00:00:00Zhttps://www.dovepress.com/bioglass-implant-coating-interactions-in-synthetic-physiological-fluid-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013AC Popa,1,2 GE Stan,1 MA Husanu,1 I Mercioniu,1 LF Santos,3 HR Fernandes,4 JMF Ferreira4 1National Institute of Materials Physics, Măgurele, 2Army Centre for Medical Research, Bucharest, Romania; 3Centro de Química Estrutural, Instituto Superior Técnico (CQE-IST), University of Lisbon, Lisbon, 4Department of Materials and Ceramics Engineering, Centre for Research in Ceramics and Composite Materials (CICECO), University of Aveiro, Aveiro, Portugal Abstract: Synthetic physiological fluids are currently used as a first in vitro bioactivity assessment for bone grafts. Our understanding about the interactions taking place at the fluid–implant interface has evolved remarkably during the last decade, and does not comply with the traditional International Organization for Standardization/final draft International Standard 23317 protocol in purely inorganic simulated body fluid. The advances in our knowledge point to the need of a true paradigm shift toward testing physiological fluids with enhanced biomimicry and a better understanding of the materials’ structure-dissolution behavior. This will contribute to “upgrade” our vision of entire cascades of events taking place at the implant surfaces upon immersion in the testing media or after implantation. Starting from an osteoinductive bioglass composition with the ability to alleviate the oxidative stress, thin bioglass films with different degrees of polymerization were deposited onto titanium substrates. Their biomineralization activity in simulated body fluid and in a series of new inorganic–organic media with increasing biomimicry that more closely simulated the human intercellular environment was compared. A comprehensive range of advanced characterization tools (scanning electron microscopy; grazing-incidence X-ray diffraction; Fourier-transform infrared, micro-Raman, energy-dispersive, X-ray photoelectron, and surface-enhanced laser desorption/ionization time-of-flight mass spectroscopies; and cytocompatibility assays using mesenchymal stem cells) were used. The information gathered is very useful to biologists, biophysicists, clinicians, and material scientists with special interest in teaching and research. By combining all the analyses, we propose herein a step forward toward establishing an improved unified protocol for testing the bioactivity of implant materials. Keywords: biomaterials, bioglass, in vitro biomimetic assays, proteinsPopa ACStan GEHusanu MAMercioniu ISantos LFFernandes HRFerreira JMFDove Medical Pressarticlebiomaterialsbioglassin vitro biomimetic assaysproteins.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 683-707 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	biomaterials bioglass in vitro biomimetic assays proteins. Medicine (General) R5-920
spellingShingle	biomaterials bioglass in vitro biomimetic assays proteins. Medicine (General) R5-920 Popa AC Stan GE Husanu MA Mercioniu I Santos LF Fernandes HR Ferreira JMF Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry
description	AC Popa,1,2 GE Stan,1 MA Husanu,1 I Mercioniu,1 LF Santos,3 HR Fernandes,4 JMF Ferreira4 1National Institute of Materials Physics, Măgurele, 2Army Centre for Medical Research, Bucharest, Romania; 3Centro de Química Estrutural, Instituto Superior Técnico (CQE-IST), University of Lisbon, Lisbon, 4Department of Materials and Ceramics Engineering, Centre for Research in Ceramics and Composite Materials (CICECO), University of Aveiro, Aveiro, Portugal Abstract: Synthetic physiological fluids are currently used as a first in vitro bioactivity assessment for bone grafts. Our understanding about the interactions taking place at the fluid–implant interface has evolved remarkably during the last decade, and does not comply with the traditional International Organization for Standardization/final draft International Standard 23317 protocol in purely inorganic simulated body fluid. The advances in our knowledge point to the need of a true paradigm shift toward testing physiological fluids with enhanced biomimicry and a better understanding of the materials’ structure-dissolution behavior. This will contribute to “upgrade” our vision of entire cascades of events taking place at the implant surfaces upon immersion in the testing media or after implantation. Starting from an osteoinductive bioglass composition with the ability to alleviate the oxidative stress, thin bioglass films with different degrees of polymerization were deposited onto titanium substrates. Their biomineralization activity in simulated body fluid and in a series of new inorganic–organic media with increasing biomimicry that more closely simulated the human intercellular environment was compared. A comprehensive range of advanced characterization tools (scanning electron microscopy; grazing-incidence X-ray diffraction; Fourier-transform infrared, micro-Raman, energy-dispersive, X-ray photoelectron, and surface-enhanced laser desorption/ionization time-of-flight mass spectroscopies; and cytocompatibility assays using mesenchymal stem cells) were used. The information gathered is very useful to biologists, biophysicists, clinicians, and material scientists with special interest in teaching and research. By combining all the analyses, we propose herein a step forward toward establishing an improved unified protocol for testing the bioactivity of implant materials. Keywords: biomaterials, bioglass, in vitro biomimetic assays, proteins
format	article
author	Popa AC Stan GE Husanu MA Mercioniu I Santos LF Fernandes HR Ferreira JMF
author_facet	Popa AC Stan GE Husanu MA Mercioniu I Santos LF Fernandes HR Ferreira JMF
author_sort	Popa AC
title	Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry
title_short	Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry
title_full	Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry
title_fullStr	Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry
title_full_unstemmed	Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry
title_sort	bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/91b8bb6f6be845f3801c4859840705a2
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Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

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