A new method to investigate the catalytic mechanism of YhdE pyrophosphatase by using a pyrophosphate fluorescence probe
Abstract YhdE is a Maf (multicopy associated filamentation) proteins from Escherichia coli which exhibits pyrophosphatase activity towards selected nucleotides, although its catalytic mechanism remains unclear. Herein we used a novel fluorescence probe (4-isoACBA–Zn(II) complex) to characterize the...
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Main Authors: | Qingya Shen, Hongwei Tan, Guo-wen Xing, Jimin Zheng, Zongchao Jia |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2017
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Online Access: | https://doaj.org/article/91bceb3d4a0e41c5ac21ecf6090b2147 |
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