Tailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization
Ibrahim Elsayed,1,2 Sinar Sayed1 1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 2Department of Pharmaceutical Sciences, College of Pharmacy, Gulf Medical University, Ajman, United Arab Emirates Abstract: Ocular drug delivery systems suffe...
Guardado en:
| Autores principales: | , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/91c5f2d3514f49ee85de927f44d3b545 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:91c5f2d3514f49ee85de927f44d3b545 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:91c5f2d3514f49ee85de927f44d3b5452021-12-02T00:35:52ZTailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization1178-2013https://doaj.org/article/91c5f2d3514f49ee85de927f44d3b5452017-10-01T00:00:00Zhttps://www.dovepress.com/tailored-nanostructured-platforms-for-boosting-transcorneal-permeation-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ibrahim Elsayed,1,2 Sinar Sayed1 1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 2Department of Pharmaceutical Sciences, College of Pharmacy, Gulf Medical University, Ajman, United Arab Emirates Abstract: Ocular drug delivery systems suffer from rapid drainage, intractable corneal permeation and short dosing intervals. Transcorneal drug permeation could increase the drug availability and efficiency in the aqueous humor. The aim of this study was to develop and optimize nanostructured formulations to provide accurate doses, long contact time and enhanced drug permeation. Nanovesicles were designed based on Box–Behnken model and prepared using the thin film hydration technique. The formed nanodispersions were evaluated by measuring the particle size, polydispersity index, zeta potential, entrapment efficiency and gelation temperature. The obtained desirability values were utilized to develop an optimized nanostructured in situ gel and insert. The optimized formulations were imaged by transmission and scanning electron microscopes. In addition, rheological characters, in vitro drug diffusion, ex vivo and in vivo permeation and safety of the optimized formulation were investigated. The optimized insert formulation was found to have a relatively lower viscosity, higher diffusion, ex vivo and in vivo permeation, when compared to the optimized in situ gel. So, the lyophilized nanostructured insert could be considered as a promising carrier and transporter for drugs across the cornea with high biocompatibility and effectiveness. Keywords: thin-film hydration, in situ, insert, confocal, transcorneal, tenoxicamElsayed ISayed SDove Medical PressarticleNanostructuredin situinsertconfocaltranscornealtenoxicam.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7947-7962 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Nanostructured in situ insert confocal transcorneal tenoxicam. Medicine (General) R5-920 |
| spellingShingle |
Nanostructured in situ insert confocal transcorneal tenoxicam. Medicine (General) R5-920 Elsayed I Sayed S Tailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization |
| description |
Ibrahim Elsayed,1,2 Sinar Sayed1 1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 2Department of Pharmaceutical Sciences, College of Pharmacy, Gulf Medical University, Ajman, United Arab Emirates Abstract: Ocular drug delivery systems suffer from rapid drainage, intractable corneal permeation and short dosing intervals. Transcorneal drug permeation could increase the drug availability and efficiency in the aqueous humor. The aim of this study was to develop and optimize nanostructured formulations to provide accurate doses, long contact time and enhanced drug permeation. Nanovesicles were designed based on Box–Behnken model and prepared using the thin film hydration technique. The formed nanodispersions were evaluated by measuring the particle size, polydispersity index, zeta potential, entrapment efficiency and gelation temperature. The obtained desirability values were utilized to develop an optimized nanostructured in situ gel and insert. The optimized formulations were imaged by transmission and scanning electron microscopes. In addition, rheological characters, in vitro drug diffusion, ex vivo and in vivo permeation and safety of the optimized formulation were investigated. The optimized insert formulation was found to have a relatively lower viscosity, higher diffusion, ex vivo and in vivo permeation, when compared to the optimized in situ gel. So, the lyophilized nanostructured insert could be considered as a promising carrier and transporter for drugs across the cornea with high biocompatibility and effectiveness. Keywords: thin-film hydration, in situ, insert, confocal, transcorneal, tenoxicam |
| format |
article |
| author |
Elsayed I Sayed S |
| author_facet |
Elsayed I Sayed S |
| author_sort |
Elsayed I |
| title |
Tailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization |
| title_short |
Tailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization |
| title_full |
Tailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization |
| title_fullStr |
Tailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization |
| title_full_unstemmed |
Tailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization |
| title_sort |
tailored nanostructured platforms for boosting transcorneal permeation: box–behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/91c5f2d3514f49ee85de927f44d3b545 |
| work_keys_str_mv |
AT elsayedi tailorednanostructuredplatformsforboostingtranscornealpermeationboxndashbehnkenstatisticaloptimizationcomprehensiveinvitroexvivoandinvivocharacterization AT sayeds tailorednanostructuredplatformsforboostingtranscornealpermeationboxndashbehnkenstatisticaloptimizationcomprehensiveinvitroexvivoandinvivocharacterization |
| _version_ |
1718403631528542208 |