Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus

Abstract. Rahayuwati S, Kusumah YM, Prawirosukarto S, Dadang, Santoso T. 2020. Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus. Biodiversitas 21: 2047-2055. Oryctes rhinoceros nudivirus (OrNV) is known as a successful classical biological control agen...

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Autores principales:	Sat Rahayuwati, Yayi Munara Kusumah, Sudharto Prawirosukarto, Dadang, Teguh Santoso
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	dna polymerase gene, genetic variability, nudiviridae family, oryctes rhinoceros nudivirus, ornv Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/91c969d412b14c23883e687b4d76ac3b
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spelling	oai:doaj.org-article:91c969d412b14c23883e687b4d76ac3b2021-11-22T00:35:33ZGenetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus1412-033X2085-472210.13057/biodiv/d210530https://doaj.org/article/91c969d412b14c23883e687b4d76ac3b2020-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5203https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Rahayuwati S, Kusumah YM, Prawirosukarto S, Dadang, Santoso T. 2020. Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus. Biodiversitas 21: 2047-2055. Oryctes rhinoceros nudivirus (OrNV) is known as a successful classical biological control agent. Recent reports have revealed differences in pathogenicity among OrNV isolates, with correlation suspected between virus virulence and genetic variability. The aim of the research is to study the genetic variability of OrNV isolates based on the DNA polymerase (DNA pol) gene and their phylogenetic relationship with Genus Alphanudivirus. Oryctes rhinoceros (Coleoptera: Scarabaeidae) as an OrNV host were collected from Sumatra, Belitung, Java, Kalimantan, and Sulawesi. Primers were designed to amplify complete gene of DNA pol. Sequencing, phylogenetic, and investigate genetic diversity scores were used to determine variability at nucleotide level. Five primers successfully amplified the entire DNA pol gene. Genetic variability of OrNV was high, from nine isolates found eight genetically different. Isolates could be divided into two groups of genetic diversity: high and low. High genetic diversity of OrNV supposed as an old population that occupied Sundaland and Wallacean paleogeography at first appearance in Miocene Era until early Pliocene 5 Mya. New population of OrNV had low genetic diversity were travel cross-island that supposed to correlate with oil palm plantation development and the genetic drift. The benefit information of the OrNV genetic variability is as preliminary guideline for virulence test. OrNV is a member of Nudiviridae family, Genus Alphanudivirus, and at recent moment they consist of eight species. Alphanudivirus infect Coleoptera, Diptera, Hemiptera and Orthoptera order that soil as immature niche may explain their closely related taxa.Sat RahayuwatiYayi Munara KusumahSudharto PrawirosukartoDadangTeguh SantosoMBI & UNS Soloarticledna polymerase gene, genetic variability, nudiviridae family, oryctes rhinoceros nudivirus, ornvBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	dna polymerase gene, genetic variability, nudiviridae family, oryctes rhinoceros nudivirus, ornv Biology (General) QH301-705.5
spellingShingle	dna polymerase gene, genetic variability, nudiviridae family, oryctes rhinoceros nudivirus, ornv Biology (General) QH301-705.5 Sat Rahayuwati Yayi Munara Kusumah Sudharto Prawirosukarto Dadang Teguh Santoso Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus
description	Abstract. Rahayuwati S, Kusumah YM, Prawirosukarto S, Dadang, Santoso T. 2020. Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus. Biodiversitas 21: 2047-2055. Oryctes rhinoceros nudivirus (OrNV) is known as a successful classical biological control agent. Recent reports have revealed differences in pathogenicity among OrNV isolates, with correlation suspected between virus virulence and genetic variability. The aim of the research is to study the genetic variability of OrNV isolates based on the DNA polymerase (DNA pol) gene and their phylogenetic relationship with Genus Alphanudivirus. Oryctes rhinoceros (Coleoptera: Scarabaeidae) as an OrNV host were collected from Sumatra, Belitung, Java, Kalimantan, and Sulawesi. Primers were designed to amplify complete gene of DNA pol. Sequencing, phylogenetic, and investigate genetic diversity scores were used to determine variability at nucleotide level. Five primers successfully amplified the entire DNA pol gene. Genetic variability of OrNV was high, from nine isolates found eight genetically different. Isolates could be divided into two groups of genetic diversity: high and low. High genetic diversity of OrNV supposed as an old population that occupied Sundaland and Wallacean paleogeography at first appearance in Miocene Era until early Pliocene 5 Mya. New population of OrNV had low genetic diversity were travel cross-island that supposed to correlate with oil palm plantation development and the genetic drift. The benefit information of the OrNV genetic variability is as preliminary guideline for virulence test. OrNV is a member of Nudiviridae family, Genus Alphanudivirus, and at recent moment they consist of eight species. Alphanudivirus infect Coleoptera, Diptera, Hemiptera and Orthoptera order that soil as immature niche may explain their closely related taxa.
format	article
author	Sat Rahayuwati Yayi Munara Kusumah Sudharto Prawirosukarto Dadang Teguh Santoso
author_facet	Sat Rahayuwati Yayi Munara Kusumah Sudharto Prawirosukarto Dadang Teguh Santoso
author_sort	Sat Rahayuwati
title	Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus
title_short	Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus
title_full	Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus
title_fullStr	Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus
title_full_unstemmed	Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus
title_sort	genetic variability of indonesian oryctes rhinoceros nudivirus (ornv) as genus of alphanudivirus
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/91c969d412b14c23883e687b4d76ac3b
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Genetic variability of Indonesian Oryctes rhinoceros nudivirus (OrNV) as genus of Alphanudivirus

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