In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.

Medical treatment using non-thermal atmospheric pressure plasma (NTAPP) is rapidly gaining recognition. NTAPP is thought to be a new therapeutic method because it could generate highly reactive species in an ambient atmosphere which could be exposed to biological targets (e.g., cells and tissues). I...

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Autores principales:	Akiyoshi Shimatani, Hiromitsu Toyoda, Kumi Orita, Yoshihiro Hirakawa, Kodai Aoki, Jun-Seok Oh, Tatsuru Shirafuji, Hiroaki Nakamura
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/91debacda1c64b799f2f735534945741
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spelling	oai:doaj.org-article:91debacda1c64b799f2f7355349457412021-12-02T20:13:45ZIn vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.1932-620310.1371/journal.pone.0255861https://doaj.org/article/91debacda1c64b799f2f7355349457412021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255861https://doaj.org/toc/1932-6203Medical treatment using non-thermal atmospheric pressure plasma (NTAPP) is rapidly gaining recognition. NTAPP is thought to be a new therapeutic method because it could generate highly reactive species in an ambient atmosphere which could be exposed to biological targets (e.g., cells and tissues). If plasma-generated reactive species could stimulate bone regeneration, NTAPP can provide a new treatment opportunity in regenerative medicine. Here, we investigated the impact of NTAPP on bone regeneration using a large bone defect in New Zealand White rabbits and a simple atmospheric pressure plasma (helium microplasma jet). We observed the recovery progress of the large bone defects by X-ray imaging over eight weeks after surgery. The X-ray results showed a clear difference in the occupancy of the new bone of the large bone defect among groups with different plasma treatment times, whereas the new bone occupancy was not substantial in the untreated control group. According to the results of micro-computed tomography analysis at eight weeks, the most successful bone regeneration was achieved using a plasma treatment time of 10 min, wherein the new bone volume was 1.51 times larger than that in the plasma untreated control group. Using H&E and Masson trichrome stains, nucleated cells were uniformly observed, and no inclusion was confirmed, respectively, in the groups of plasma treatment. We concluded the critical large bone defect were filled with new bone. Overall, these results suggest that NTAPP is promising for fracture treatment.Akiyoshi ShimataniHiromitsu ToyodaKumi OritaYoshihiro HirakawaKodai AokiJun-Seok OhTatsuru ShirafujiHiroaki NakamuraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0255861 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akiyoshi Shimatani Hiromitsu Toyoda Kumi Orita Yoshihiro Hirakawa Kodai Aoki Jun-Seok Oh Tatsuru Shirafuji Hiroaki Nakamura In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.
description	Medical treatment using non-thermal atmospheric pressure plasma (NTAPP) is rapidly gaining recognition. NTAPP is thought to be a new therapeutic method because it could generate highly reactive species in an ambient atmosphere which could be exposed to biological targets (e.g., cells and tissues). If plasma-generated reactive species could stimulate bone regeneration, NTAPP can provide a new treatment opportunity in regenerative medicine. Here, we investigated the impact of NTAPP on bone regeneration using a large bone defect in New Zealand White rabbits and a simple atmospheric pressure plasma (helium microplasma jet). We observed the recovery progress of the large bone defects by X-ray imaging over eight weeks after surgery. The X-ray results showed a clear difference in the occupancy of the new bone of the large bone defect among groups with different plasma treatment times, whereas the new bone occupancy was not substantial in the untreated control group. According to the results of micro-computed tomography analysis at eight weeks, the most successful bone regeneration was achieved using a plasma treatment time of 10 min, wherein the new bone volume was 1.51 times larger than that in the plasma untreated control group. Using H&E and Masson trichrome stains, nucleated cells were uniformly observed, and no inclusion was confirmed, respectively, in the groups of plasma treatment. We concluded the critical large bone defect were filled with new bone. Overall, these results suggest that NTAPP is promising for fracture treatment.
format	article
author	Akiyoshi Shimatani Hiromitsu Toyoda Kumi Orita Yoshihiro Hirakawa Kodai Aoki Jun-Seok Oh Tatsuru Shirafuji Hiroaki Nakamura
author_facet	Akiyoshi Shimatani Hiromitsu Toyoda Kumi Orita Yoshihiro Hirakawa Kodai Aoki Jun-Seok Oh Tatsuru Shirafuji Hiroaki Nakamura
author_sort	Akiyoshi Shimatani
title	In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.
title_short	In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.
title_full	In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.
title_fullStr	In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.
title_full_unstemmed	In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.
title_sort	in vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/91debacda1c64b799f2f735534945741
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In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma.

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