Experimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics

Context. The wettability of the chemically modified dentin substrate is a condition that intervenes in dentin-vascular and cellular interaction across regenerative endodontics. Aims. To compare the effect of CS and CSnp on the wettability in root dentine with other irrigation protocols with an exper...

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Autores principales:	Fernando Arias Alvarado, Maira Rivero Iriarte, Freddy Jordan Mariño, Sara Quijano-Guauque, León D. Pérez, Yolima Baena, Claudia García-Guerrero
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/91e69b6afe2c432bb4d600b9b5a37418
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spelling	oai:doaj.org-article:91e69b6afe2c432bb4d600b9b5a374182021-11-08T02:35:56ZExperimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics1687-879510.1155/2021/8772706https://doaj.org/article/91e69b6afe2c432bb4d600b9b5a374182021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/8772706https://doaj.org/toc/1687-8795Context. The wettability of the chemically modified dentin substrate is a condition that intervenes in dentin-vascular and cellular interaction across regenerative endodontics. Aims. To compare the effect of CS and CSnp on the wettability in root dentine with other irrigation protocols with an experimental in vitro model prior regenerative endodontics. Methods and Material. An in vitro experimental study that included eighty hemisected human root distributed into 8 groups: G1- distilled water; G2- 1% NaOCl/17% EDTA; G3- hypochlorous acid 0.025% HOCl, G4- 1% NaOCl/0.025% HOCl/17% EDTA, G5- 0.2 g/100 mL CS, G6- 1% NaOCl/0.2 g/100 mL CS, G7- CSnp, and G8- 1% NaOCl/CSnp. The wettability analysis calculated the contact angle (θ) between a drop of a blood-like and root dentinal surface; topographic characterization with scanning electron microscopy (SEM) quantified the diameter and number of tubules per area; spectroscopy infrared analyses (IR-S) identified chemical changes in the inorganic (phosphate/carbonate) and organic phase (amide/methyl). Statistical analysis: a linear mixed model, Kruskal–Wallis, and Holm–Bonferroni correction (P < 0.05) were used. Results. Significantly higher wettability for G2 (27.1 (P = 0.0001)) was found. A mean value of 67°±°for experimental groups (P = 0.07) was found, and we did not identify differences between them. The SEM identified greater tubular opening and erosion for G4 and greater dentinal permeability per area for NaOCl/CS. IR-S identified dentinal organic integrity with NaOCl-CS/CSnp compared to organic reduction promoted for NaOCl/EDTA. Conclusions. This in vitro dentin determined an indirect association between the wettability and organic contents. The oxidative effect of NaOCl could be neutralized by CS-CSnp, and consequently, the wettability of the substrate decreases.Fernando Arias AlvaradoMaira Rivero IriarteFreddy Jordan MariñoSara Quijano-GuauqueLeón D. PérezYolima BaenaClaudia García-GuerreroHindawi LimitedarticleBiotechnologyTP248.13-248.65ENInternational Journal of Biomaterials, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Fernando Arias Alvarado Maira Rivero Iriarte Freddy Jordan Mariño Sara Quijano-Guauque León D. Pérez Yolima Baena Claudia García-Guerrero Experimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics
description	Context. The wettability of the chemically modified dentin substrate is a condition that intervenes in dentin-vascular and cellular interaction across regenerative endodontics. Aims. To compare the effect of CS and CSnp on the wettability in root dentine with other irrigation protocols with an experimental in vitro model prior regenerative endodontics. Methods and Material. An in vitro experimental study that included eighty hemisected human root distributed into 8 groups: G1- distilled water; G2- 1% NaOCl/17% EDTA; G3- hypochlorous acid 0.025% HOCl, G4- 1% NaOCl/0.025% HOCl/17% EDTA, G5- 0.2 g/100 mL CS, G6- 1% NaOCl/0.2 g/100 mL CS, G7- CSnp, and G8- 1% NaOCl/CSnp. The wettability analysis calculated the contact angle (θ) between a drop of a blood-like and root dentinal surface; topographic characterization with scanning electron microscopy (SEM) quantified the diameter and number of tubules per area; spectroscopy infrared analyses (IR-S) identified chemical changes in the inorganic (phosphate/carbonate) and organic phase (amide/methyl). Statistical analysis: a linear mixed model, Kruskal–Wallis, and Holm–Bonferroni correction (P < 0.05) were used. Results. Significantly higher wettability for G2 (27.1 (P = 0.0001)) was found. A mean value of 67°±°for experimental groups (P = 0.07) was found, and we did not identify differences between them. The SEM identified greater tubular opening and erosion for G4 and greater dentinal permeability per area for NaOCl/CS. IR-S identified dentinal organic integrity with NaOCl-CS/CSnp compared to organic reduction promoted for NaOCl/EDTA. Conclusions. This in vitro dentin determined an indirect association between the wettability and organic contents. The oxidative effect of NaOCl could be neutralized by CS-CSnp, and consequently, the wettability of the substrate decreases.
format	article
author	Fernando Arias Alvarado Maira Rivero Iriarte Freddy Jordan Mariño Sara Quijano-Guauque León D. Pérez Yolima Baena Claudia García-Guerrero
author_facet	Fernando Arias Alvarado Maira Rivero Iriarte Freddy Jordan Mariño Sara Quijano-Guauque León D. Pérez Yolima Baena Claudia García-Guerrero
author_sort	Fernando Arias Alvarado
title	Experimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics
title_short	Experimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics
title_full	Experimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics
title_fullStr	Experimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics
title_full_unstemmed	Experimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics
title_sort	experimental solution of chitosan and nanochitosan on wettability in root dentine: in vitro model prior regenerative endodontics
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/91e69b6afe2c432bb4d600b9b5a37418
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Experimental Solution of Chitosan and Nanochitosan on Wettability in Root Dentine: In Vitro Model Prior Regenerative Endodontics

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