Adaptation in a Fibronectin Binding Autolysin of <named-content content-type="genus-species">Staphylococcus saprophyticus</named-content>

ABSTRACT Human-pathogenic bacteria are found in a variety of niches, including free-living, zoonotic, and microbiome environments. Identifying bacterial adaptations that enable invasive disease is an important means of gaining insight into the molecular basis of pathogenesis and understanding pathog...

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Autores principales: Tatum D. Mortimer, Douglas S. Annis, Mary B. O’Neill, Lindsey L. Bohr, Tracy M. Smith, Hendrik N. Poinar, Deane F. Mosher, Caitlin S. Pepperell
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Publicado: American Society for Microbiology 2017
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spelling oai:doaj.org-article:91e797bf65b746edb92975a1a0a9391a2021-11-15T15:21:52ZAdaptation in a Fibronectin Binding Autolysin of <named-content content-type="genus-species">Staphylococcus saprophyticus</named-content>10.1128/mSphere.00511-172379-5042https://doaj.org/article/91e797bf65b746edb92975a1a0a9391a2017-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00511-17https://doaj.org/toc/2379-5042ABSTRACT Human-pathogenic bacteria are found in a variety of niches, including free-living, zoonotic, and microbiome environments. Identifying bacterial adaptations that enable invasive disease is an important means of gaining insight into the molecular basis of pathogenesis and understanding pathogen emergence. Staphylococcus saprophyticus, a leading cause of urinary tract infections, can be found in the environment, food, animals, and the human microbiome. We identified a selective sweep in the gene encoding the Aas adhesin, a key virulence factor that binds host fibronectin. We hypothesize that the mutation under selection (aas_2206A>C) facilitates colonization of the urinary tract, an environment where bacteria are subject to strong shearing forces. The mutation appears to have enabled emergence and expansion of a human-pathogenic lineage of S. saprophyticus. These results demonstrate the power of evolutionary genomic approaches in discovering the genetic basis of virulence and emphasize the pleiotropy and adaptability of bacteria occupying diverse niches. IMPORTANCE Staphylococcus saprophyticus is an important cause of urinary tract infections (UTI) in women; such UTI are common, can be severe, and are associated with significant impacts to public health. In addition to being a cause of human UTI, S. saprophyticus can be found in the environment, in food, and associated with animals. After discovering that UTI strains of S. saprophyticus are for the most part closely related to each other, we sought to determine whether these strains are specially adapted to cause disease in humans. We found evidence suggesting that a mutation in the gene aas is advantageous in the context of human infection. We hypothesize that the mutation allows S. saprophyticus to survive better in the human urinary tract. These results show how bacteria found in the environment can evolve to cause disease.Tatum D. MortimerDouglas S. AnnisMary B. O’NeillLindsey L. BohrTracy M. SmithHendrik N. PoinarDeane F. MosherCaitlin S. PepperellAmerican Society for MicrobiologyarticleStaphylococcus saprophyticusadhesinsevolutionpositive selectionurinary tract infectionMicrobiologyQR1-502ENmSphere, Vol 2, Iss 6 (2017)
institution DOAJ
collection DOAJ
language EN
topic Staphylococcus saprophyticus
adhesins
evolution
positive selection
urinary tract infection
Microbiology
QR1-502
spellingShingle Staphylococcus saprophyticus
adhesins
evolution
positive selection
urinary tract infection
Microbiology
QR1-502
Tatum D. Mortimer
Douglas S. Annis
Mary B. O’Neill
Lindsey L. Bohr
Tracy M. Smith
Hendrik N. Poinar
Deane F. Mosher
Caitlin S. Pepperell
Adaptation in a Fibronectin Binding Autolysin of <named-content content-type="genus-species">Staphylococcus saprophyticus</named-content>
description ABSTRACT Human-pathogenic bacteria are found in a variety of niches, including free-living, zoonotic, and microbiome environments. Identifying bacterial adaptations that enable invasive disease is an important means of gaining insight into the molecular basis of pathogenesis and understanding pathogen emergence. Staphylococcus saprophyticus, a leading cause of urinary tract infections, can be found in the environment, food, animals, and the human microbiome. We identified a selective sweep in the gene encoding the Aas adhesin, a key virulence factor that binds host fibronectin. We hypothesize that the mutation under selection (aas_2206A>C) facilitates colonization of the urinary tract, an environment where bacteria are subject to strong shearing forces. The mutation appears to have enabled emergence and expansion of a human-pathogenic lineage of S. saprophyticus. These results demonstrate the power of evolutionary genomic approaches in discovering the genetic basis of virulence and emphasize the pleiotropy and adaptability of bacteria occupying diverse niches. IMPORTANCE Staphylococcus saprophyticus is an important cause of urinary tract infections (UTI) in women; such UTI are common, can be severe, and are associated with significant impacts to public health. In addition to being a cause of human UTI, S. saprophyticus can be found in the environment, in food, and associated with animals. After discovering that UTI strains of S. saprophyticus are for the most part closely related to each other, we sought to determine whether these strains are specially adapted to cause disease in humans. We found evidence suggesting that a mutation in the gene aas is advantageous in the context of human infection. We hypothesize that the mutation allows S. saprophyticus to survive better in the human urinary tract. These results show how bacteria found in the environment can evolve to cause disease.
format article
author Tatum D. Mortimer
Douglas S. Annis
Mary B. O’Neill
Lindsey L. Bohr
Tracy M. Smith
Hendrik N. Poinar
Deane F. Mosher
Caitlin S. Pepperell
author_facet Tatum D. Mortimer
Douglas S. Annis
Mary B. O’Neill
Lindsey L. Bohr
Tracy M. Smith
Hendrik N. Poinar
Deane F. Mosher
Caitlin S. Pepperell
author_sort Tatum D. Mortimer
title Adaptation in a Fibronectin Binding Autolysin of <named-content content-type="genus-species">Staphylococcus saprophyticus</named-content>
title_short Adaptation in a Fibronectin Binding Autolysin of <named-content content-type="genus-species">Staphylococcus saprophyticus</named-content>
title_full Adaptation in a Fibronectin Binding Autolysin of <named-content content-type="genus-species">Staphylococcus saprophyticus</named-content>
title_fullStr Adaptation in a Fibronectin Binding Autolysin of <named-content content-type="genus-species">Staphylococcus saprophyticus</named-content>
title_full_unstemmed Adaptation in a Fibronectin Binding Autolysin of <named-content content-type="genus-species">Staphylococcus saprophyticus</named-content>
title_sort adaptation in a fibronectin binding autolysin of <named-content content-type="genus-species">staphylococcus saprophyticus</named-content>
publisher American Society for Microbiology
publishDate 2017
url https://doaj.org/article/91e797bf65b746edb92975a1a0a9391a
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