Highly efficient baculovirus-mediated multigene delivery in primary cells

Current viral gene delivery systems are limited in the amount of foreign DNA they can deliver to cells. Here the authors develop MultiPrime, a baculovirus-based vector system capable of multigene delivery into a wide variety of cells, and use Multiprime for genome engineering by CRISPR/Cas9.

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Autores principales:	Maysam Mansouri, Itxaso Bellon-Echeverria, Aurélien Rizk, Zahra Ehsaei, Chiara Cianciolo Cosentino, Catarina S. Silva, Ye Xie, Frederick M. Boyce, M. Wayne Davis, Stephan C. F. Neuhauss, Verdon Taylor, Kurt Ballmer-Hofer, Imre Berger, Philipp Berger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/91e7d81de6ed4df8941fdab73f25c864
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spelling	oai:doaj.org-article:91e7d81de6ed4df8941fdab73f25c8642021-12-02T15:35:22ZHighly efficient baculovirus-mediated multigene delivery in primary cells10.1038/ncomms115292041-1723https://doaj.org/article/91e7d81de6ed4df8941fdab73f25c8642016-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms11529https://doaj.org/toc/2041-1723Current viral gene delivery systems are limited in the amount of foreign DNA they can deliver to cells. Here the authors develop MultiPrime, a baculovirus-based vector system capable of multigene delivery into a wide variety of cells, and use Multiprime for genome engineering by CRISPR/Cas9.Maysam MansouriItxaso Bellon-EcheverriaAurélien RizkZahra EhsaeiChiara Cianciolo CosentinoCatarina S. SilvaYe XieFrederick M. BoyceM. Wayne DavisStephan C. F. NeuhaussVerdon TaylorKurt Ballmer-HoferImre BergerPhilipp BergerNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-13 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Maysam Mansouri Itxaso Bellon-Echeverria Aurélien Rizk Zahra Ehsaei Chiara Cianciolo Cosentino Catarina S. Silva Ye Xie Frederick M. Boyce M. Wayne Davis Stephan C. F. Neuhauss Verdon Taylor Kurt Ballmer-Hofer Imre Berger Philipp Berger Highly efficient baculovirus-mediated multigene delivery in primary cells
description	Current viral gene delivery systems are limited in the amount of foreign DNA they can deliver to cells. Here the authors develop MultiPrime, a baculovirus-based vector system capable of multigene delivery into a wide variety of cells, and use Multiprime for genome engineering by CRISPR/Cas9.
format	article
author	Maysam Mansouri Itxaso Bellon-Echeverria Aurélien Rizk Zahra Ehsaei Chiara Cianciolo Cosentino Catarina S. Silva Ye Xie Frederick M. Boyce M. Wayne Davis Stephan C. F. Neuhauss Verdon Taylor Kurt Ballmer-Hofer Imre Berger Philipp Berger
author_facet	Maysam Mansouri Itxaso Bellon-Echeverria Aurélien Rizk Zahra Ehsaei Chiara Cianciolo Cosentino Catarina S. Silva Ye Xie Frederick M. Boyce M. Wayne Davis Stephan C. F. Neuhauss Verdon Taylor Kurt Ballmer-Hofer Imre Berger Philipp Berger
author_sort	Maysam Mansouri
title	Highly efficient baculovirus-mediated multigene delivery in primary cells
title_short	Highly efficient baculovirus-mediated multigene delivery in primary cells
title_full	Highly efficient baculovirus-mediated multigene delivery in primary cells
title_fullStr	Highly efficient baculovirus-mediated multigene delivery in primary cells
title_full_unstemmed	Highly efficient baculovirus-mediated multigene delivery in primary cells
title_sort	highly efficient baculovirus-mediated multigene delivery in primary cells
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/91e7d81de6ed4df8941fdab73f25c864
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Highly efficient baculovirus-mediated multigene delivery in primary cells

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