Highly efficient baculovirus-mediated multigene delivery in primary cells
Current viral gene delivery systems are limited in the amount of foreign DNA they can deliver to cells. Here the authors develop MultiPrime, a baculovirus-based vector system capable of multigene delivery into a wide variety of cells, and use Multiprime for genome engineering by CRISPR/Cas9.
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Auteurs principaux: | Maysam Mansouri, Itxaso Bellon-Echeverria, Aurélien Rizk, Zahra Ehsaei, Chiara Cianciolo Cosentino, Catarina S. Silva, Ye Xie, Frederick M. Boyce, M. Wayne Davis, Stephan C. F. Neuhauss, Verdon Taylor, Kurt Ballmer-Hofer, Imre Berger, Philipp Berger |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2016
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Sujets: | |
Accès en ligne: | https://doaj.org/article/91e7d81de6ed4df8941fdab73f25c864 |
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