Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves

Abstract. Suryanti V, Kusumaningsih T, Marliyana SD, Setyono HA, Trisnawati EW. 2020. Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves. Biodiversitas 21: 941-947. Teak (Tectona grandis) grows in tropical area and the teak wood is widely used for building c...

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Autores principales:	Venty Suryanti, TRIANA KUSUMANINGSIH, SOERYA DEWI MARLIYANA, HILDA ALFIANI SETYONO, ELYNA WAHYU TRISNAWATI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	antioxidant activity, tectona grandis, teak leaves, total anthocyanins, total phenolics Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/91ee35de2ba8459a882af5ba74f529f6
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spelling	oai:doaj.org-article:91ee35de2ba8459a882af5ba74f529f62021-11-22T00:28:56ZIdentification of active compounds and antioxidant activity of teak (Tectona grandis) leaves1412-033X2085-472210.13057/biodiv/d210313https://doaj.org/article/91ee35de2ba8459a882af5ba74f529f62020-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5154https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Suryanti V, Kusumaningsih T, Marliyana SD, Setyono HA, Trisnawati EW. 2020. Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves. Biodiversitas 21: 941-947. Teak (Tectona grandis) grows in tropical area and the teak wood is widely used for building construction and furniture. Young teak leaf contains red pigment and often are used for natural food coloring. The research objectives were to isolate the young teak leaf chemical compounds and to investigate their antioxidant activities. The compounds identification was carried out by UV-Vis Spectroscopy, GC-MS and LC-MS. The antioxidant activity determination was carried out by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method. The total phenolics content was determined using Folin-Ciocalteu method and total anthocyanins content was examined using the H-differential method. Two fractions were observed further by UV-Vis Spectroscopy, GC-MS and LC-MS. The UV-Vis spectrum of FA confirmed the presence of anthocyanins which has maximum absorbance at 208 and 492 nm. The GC-MS spectrum of fraction A (FA) revealed 4-hydroxy-4-methyl-2-pentanone, glycerin monoacetate, glycerin diacetate and 1-eicosanol. The UV-Vis spectrum of FC also confirms the presence of anthocyanins which has maximum absorbance at 489 nm. The LC-MS spectrum of fraction C (FC) shows anthocyanins, namely malvidin-3-o- (6-o-acetyl)-5-o-diglucoside. The FA and FC teak leaf fractions have strong antioxidant activities as their IC50 values are less than 50 ppm. Total phenolic of teak leaf extract was 6.17 mg GAE/g and total anthocyanins of teak leaf extract were 67.5 mg/L.Venty SuryantiTRIANA KUSUMANINGSIHSOERYA DEWI MARLIYANAHILDA ALFIANI SETYONOELYNA WAHYU TRISNAWATIMBI & UNS Soloarticleantioxidant activity, tectona grandis, teak leaves, total anthocyanins, total phenolicsBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant activity, tectona grandis, teak leaves, total anthocyanins, total phenolics Biology (General) QH301-705.5
spellingShingle	antioxidant activity, tectona grandis, teak leaves, total anthocyanins, total phenolics Biology (General) QH301-705.5 Venty Suryanti TRIANA KUSUMANINGSIH SOERYA DEWI MARLIYANA HILDA ALFIANI SETYONO ELYNA WAHYU TRISNAWATI Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves
description	Abstract. Suryanti V, Kusumaningsih T, Marliyana SD, Setyono HA, Trisnawati EW. 2020. Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves. Biodiversitas 21: 941-947. Teak (Tectona grandis) grows in tropical area and the teak wood is widely used for building construction and furniture. Young teak leaf contains red pigment and often are used for natural food coloring. The research objectives were to isolate the young teak leaf chemical compounds and to investigate their antioxidant activities. The compounds identification was carried out by UV-Vis Spectroscopy, GC-MS and LC-MS. The antioxidant activity determination was carried out by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method. The total phenolics content was determined using Folin-Ciocalteu method and total anthocyanins content was examined using the H-differential method. Two fractions were observed further by UV-Vis Spectroscopy, GC-MS and LC-MS. The UV-Vis spectrum of FA confirmed the presence of anthocyanins which has maximum absorbance at 208 and 492 nm. The GC-MS spectrum of fraction A (FA) revealed 4-hydroxy-4-methyl-2-pentanone, glycerin monoacetate, glycerin diacetate and 1-eicosanol. The UV-Vis spectrum of FC also confirms the presence of anthocyanins which has maximum absorbance at 489 nm. The LC-MS spectrum of fraction C (FC) shows anthocyanins, namely malvidin-3-o- (6-o-acetyl)-5-o-diglucoside. The FA and FC teak leaf fractions have strong antioxidant activities as their IC50 values are less than 50 ppm. Total phenolic of teak leaf extract was 6.17 mg GAE/g and total anthocyanins of teak leaf extract were 67.5 mg/L.
format	article
author	Venty Suryanti TRIANA KUSUMANINGSIH SOERYA DEWI MARLIYANA HILDA ALFIANI SETYONO ELYNA WAHYU TRISNAWATI
author_facet	Venty Suryanti TRIANA KUSUMANINGSIH SOERYA DEWI MARLIYANA HILDA ALFIANI SETYONO ELYNA WAHYU TRISNAWATI
author_sort	Venty Suryanti
title	Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves
title_short	Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves
title_full	Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves
title_fullStr	Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves
title_full_unstemmed	Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves
title_sort	identification of active compounds and antioxidant activity of teak (tectona grandis) leaves
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/91ee35de2ba8459a882af5ba74f529f6
work_keys_str_mv	AT ventysuryanti identificationofactivecompoundsandantioxidantactivityofteaktectonagrandisleaves AT trianakusumaningsih identificationofactivecompoundsandantioxidantactivityofteaktectonagrandisleaves AT soeryadewimarliyana identificationofactivecompoundsandantioxidantactivityofteaktectonagrandisleaves AT hildaalfianisetyono identificationofactivecompoundsandantioxidantactivityofteaktectonagrandisleaves AT elynawahyutrisnawati identificationofactivecompoundsandantioxidantactivityofteaktectonagrandisleaves
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Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves

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