Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A

Abstract Expression of OCT4A is one of the hallmarks of pluripotency, defined as a stem cell’s ability to differentiate into all the lineages of the three germ layers. Despite being defined as non-tumorigenic cells with high translational potential, human mid-trimester amniotic fluid stem cells (hAF...

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Autores principales:	Filipa Vlahova, Kate E. Hawkins, Anna Maria Ranzoni, Kwan-Leong Hau, Rachel Sagar, Paolo De Coppi, Anna L. David, James Adjaye, Pascale V. Guillot
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/91eed63b43694b80bf06048c8f368e29
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spelling	oai:doaj.org-article:91eed63b43694b80bf06048c8f368e292021-12-02T15:09:19ZHuman mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A10.1038/s41598-019-44572-x2045-2322https://doaj.org/article/91eed63b43694b80bf06048c8f368e292019-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44572-xhttps://doaj.org/toc/2045-2322Abstract Expression of OCT4A is one of the hallmarks of pluripotency, defined as a stem cell’s ability to differentiate into all the lineages of the three germ layers. Despite being defined as non-tumorigenic cells with high translational potential, human mid-trimester amniotic fluid stem cells (hAFSCs) are often described as sharing features with embryonic stem cells, including the expression of OCT4A, which could hinder their clinical potential. To clarify the OCT4A status of hAFSCs, we first undertook a systematic review of the literature. We then performed extensive gene and protein expression analyses to discover that neither frozen, nor fresh hAFSCs cultivated in multipotent stem cell culture conditions expressed OCT4A, and that the OCT4A positive results from the literature are likely to be attributed to the expression of pseudogenes or other OCT4 variants. To address this issue, we provide a robust protocol for the assessment of OCT4A in other stem cells.Filipa VlahovaKate E. HawkinsAnna Maria RanzoniKwan-Leong HauRachel SagarPaolo De CoppiAnna L. DavidJames AdjayePascale V. GuillotNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Filipa Vlahova Kate E. Hawkins Anna Maria Ranzoni Kwan-Leong Hau Rachel Sagar Paolo De Coppi Anna L. David James Adjaye Pascale V. Guillot Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
description	Abstract Expression of OCT4A is one of the hallmarks of pluripotency, defined as a stem cell’s ability to differentiate into all the lineages of the three germ layers. Despite being defined as non-tumorigenic cells with high translational potential, human mid-trimester amniotic fluid stem cells (hAFSCs) are often described as sharing features with embryonic stem cells, including the expression of OCT4A, which could hinder their clinical potential. To clarify the OCT4A status of hAFSCs, we first undertook a systematic review of the literature. We then performed extensive gene and protein expression analyses to discover that neither frozen, nor fresh hAFSCs cultivated in multipotent stem cell culture conditions expressed OCT4A, and that the OCT4A positive results from the literature are likely to be attributed to the expression of pseudogenes or other OCT4 variants. To address this issue, we provide a robust protocol for the assessment of OCT4A in other stem cells.
format	article
author	Filipa Vlahova Kate E. Hawkins Anna Maria Ranzoni Kwan-Leong Hau Rachel Sagar Paolo De Coppi Anna L. David James Adjaye Pascale V. Guillot
author_facet	Filipa Vlahova Kate E. Hawkins Anna Maria Ranzoni Kwan-Leong Hau Rachel Sagar Paolo De Coppi Anna L. David James Adjaye Pascale V. Guillot
author_sort	Filipa Vlahova
title	Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
title_short	Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
title_full	Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
title_fullStr	Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
title_full_unstemmed	Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
title_sort	human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker oct4a
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/91eed63b43694b80bf06048c8f368e29
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Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A

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