Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran

Background: Rotavirus (RV) is one of the most important causes of diarrhea in the calf and human neonates. Rotaviruses are divided into nine different serogroups, of which group A is more important compared to other groups. Objective: This study was performed because of the lack of information about...

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Autores principales:	Ahmad Nazaktabar, Omid Madadgar
Formato:	article
Lenguaje:	EN
Publicado:	Alborz University of Medical Sciences 2020
Materias:	rotaviruses a rotaviruses b rotaviruses c vp6 gene phylogenetic analysis genotyping Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/91fb773f81e244c3b6484a1632409b03
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spelling	oai:doaj.org-article:91fb773f81e244c3b6484a1632409b032021-11-18T06:56:28ZPhylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran2345-33622322-586610.34172/ijep.2020.14https://doaj.org/article/91fb773f81e244c3b6484a1632409b032020-05-01T00:00:00Zhttp://enterpathog.abzums.ac.ir/PDF/ijep-4339https://doaj.org/toc/2345-3362https://doaj.org/toc/2322-5866Background: Rotavirus (RV) is one of the most important causes of diarrhea in the calf and human neonates. Rotaviruses are divided into nine different serogroups, of which group A is more important compared to other groups. Objective: This study was performed because of the lack of information about the importance and prevalence of bovine rotaviruses B (RVB) and C (RVC) and human genotypes of rotavirus A (RVA) in the bovine population in Iran. Phylogenetic analysis of VP6 of bovine RVA was the second aim of the present study. Materials and Methods: A total of 581 stool specimens were collected from diarrheic calves of 14 provinces and were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and 485 of them were investigated by PAGE electrophoresis to determine the frequency of three rotaviruses A (RVA), B (RVB), and C (RVC). The presence of human G and P genotypes in Iranian bovine population was also evaluated using semi-nested multiplex RT-PCR. Results: RVA was detected by RT-PCR (VP6 gene detection) in 16.2% (94/581) and by PAGE in 22.16% (108/485) and no positive cases of RVB and RVC were confirmed by polyacrylamide gel electrophoresis (PAGE). This study showed that non-A RV groups (B and C) have little role in calf diarrhea in Iran. The results of the phylogenetic study of VP6 sequences of rotaviruses A identified in this study showed that they all belonged to genotype I2 and were classified into three different branches. Specimen isolated in Zanjan showed the highest difference (maximum identity of 94%) with other sequences and clustered along with the Japanese strain, R22. Human G and P genotypes were not found in the studied samples. Conclusion: The results showed that non-A rotaviruses and human genotypes of RVA are of little importance in calf rotavirus diarrhea in Iran. Also, there is the first phylogenetic study of rotavirus A VP6 protein in Iran.Ahmad NazaktabarOmid MadadgarAlborz University of Medical Sciencesarticlerotaviruses a rotaviruses b rotaviruses c vp6 gene phylogenetic analysis genotypingDiseases of the digestive system. GastroenterologyRC799-869ENInternational Journal of Enteric Pathogens, Vol 8, Iss 2, Pp 66-72 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	rotaviruses a rotaviruses b rotaviruses c vp6 gene phylogenetic analysis genotyping Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	rotaviruses a rotaviruses b rotaviruses c vp6 gene phylogenetic analysis genotyping Diseases of the digestive system. Gastroenterology RC799-869 Ahmad Nazaktabar Omid Madadgar Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran
description	Background: Rotavirus (RV) is one of the most important causes of diarrhea in the calf and human neonates. Rotaviruses are divided into nine different serogroups, of which group A is more important compared to other groups. Objective: This study was performed because of the lack of information about the importance and prevalence of bovine rotaviruses B (RVB) and C (RVC) and human genotypes of rotavirus A (RVA) in the bovine population in Iran. Phylogenetic analysis of VP6 of bovine RVA was the second aim of the present study. Materials and Methods: A total of 581 stool specimens were collected from diarrheic calves of 14 provinces and were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and 485 of them were investigated by PAGE electrophoresis to determine the frequency of three rotaviruses A (RVA), B (RVB), and C (RVC). The presence of human G and P genotypes in Iranian bovine population was also evaluated using semi-nested multiplex RT-PCR. Results: RVA was detected by RT-PCR (VP6 gene detection) in 16.2% (94/581) and by PAGE in 22.16% (108/485) and no positive cases of RVB and RVC were confirmed by polyacrylamide gel electrophoresis (PAGE). This study showed that non-A RV groups (B and C) have little role in calf diarrhea in Iran. The results of the phylogenetic study of VP6 sequences of rotaviruses A identified in this study showed that they all belonged to genotype I2 and were classified into three different branches. Specimen isolated in Zanjan showed the highest difference (maximum identity of 94%) with other sequences and clustered along with the Japanese strain, R22. Human G and P genotypes were not found in the studied samples. Conclusion: The results showed that non-A rotaviruses and human genotypes of RVA are of little importance in calf rotavirus diarrhea in Iran. Also, there is the first phylogenetic study of rotavirus A VP6 protein in Iran.
format	article
author	Ahmad Nazaktabar Omid Madadgar
author_facet	Ahmad Nazaktabar Omid Madadgar
author_sort	Ahmad Nazaktabar
title	Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran
title_short	Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran
title_full	Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran
title_fullStr	Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran
title_full_unstemmed	Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran
title_sort	phylogenetic study of vp6 gene of bovine rotavirus a and molecular survey of bovine rotaviruses b and c, and human g and p genotypes of rotavirus a in calves in iran
publisher	Alborz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/91fb773f81e244c3b6484a1632409b03
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Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran

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