Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies

Abstract The ability to measure dynamic structural changes within a cell under applied load is essential for developing more accurate models of cell mechanics and mechanotransduction. Atomic force microscopy is a powerful tool for evaluating cell mechanics, but the dominant applied forces and sample...

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Autores principales:	Kellie Beicker, E. Timothy O’Brien, Michael R. Falvo, Richard Superfine
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/92046a0712794e21814f8b6b140870cc
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spelling	oai:doaj.org-article:92046a0712794e21814f8b6b140870cc2021-12-02T15:08:53ZVertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies10.1038/s41598-018-19791-32045-2322https://doaj.org/article/92046a0712794e21814f8b6b140870cc2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19791-3https://doaj.org/toc/2045-2322Abstract The ability to measure dynamic structural changes within a cell under applied load is essential for developing more accurate models of cell mechanics and mechanotransduction. Atomic force microscopy is a powerful tool for evaluating cell mechanics, but the dominant applied forces and sample strains are in the vertical direction, perpendicular to the imaging plane of standard fluorescence imaging. Here we report on a combined sideways imaging and vertical light sheet illumination system integrated with AFM. Our system enables high frame rate, low background imaging of subcellular structural dynamics in the vertical plane synchronized with AFM force data. Using our system for cell compression measurements, we correlated stiffening features in the force indentation data with onset of nuclear deformation revealed in the imaging data. In adhesion studies we were able to correlate detailed features in the force data during adhesive release events with strain at the membrane and within the nucleus.Kellie BeickerE. Timothy O’BrienMichael R. FalvoRichard SuperfineNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kellie Beicker E. Timothy O’Brien Michael R. Falvo Richard Superfine Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies
description	Abstract The ability to measure dynamic structural changes within a cell under applied load is essential for developing more accurate models of cell mechanics and mechanotransduction. Atomic force microscopy is a powerful tool for evaluating cell mechanics, but the dominant applied forces and sample strains are in the vertical direction, perpendicular to the imaging plane of standard fluorescence imaging. Here we report on a combined sideways imaging and vertical light sheet illumination system integrated with AFM. Our system enables high frame rate, low background imaging of subcellular structural dynamics in the vertical plane synchronized with AFM force data. Using our system for cell compression measurements, we correlated stiffening features in the force indentation data with onset of nuclear deformation revealed in the imaging data. In adhesion studies we were able to correlate detailed features in the force data during adhesive release events with strain at the membrane and within the nucleus.
format	article
author	Kellie Beicker E. Timothy O’Brien Michael R. Falvo Richard Superfine
author_facet	Kellie Beicker E. Timothy O’Brien Michael R. Falvo Richard Superfine
author_sort	Kellie Beicker
title	Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies
title_short	Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies
title_full	Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies
title_fullStr	Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies
title_full_unstemmed	Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies
title_sort	vertical light sheet enhanced side-view imaging for afm cell mechanics studies
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/92046a0712794e21814f8b6b140870cc
work_keys_str_mv	AT kelliebeicker verticallightsheetenhancedsideviewimagingforafmcellmechanicsstudies AT etimothyobrien verticallightsheetenhancedsideviewimagingforafmcellmechanicsstudies AT michaelrfalvo verticallightsheetenhancedsideviewimagingforafmcellmechanicsstudies AT richardsuperfine verticallightsheetenhancedsideviewimagingforafmcellmechanicsstudies
_version_	1718388014666743808

Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies

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