Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing

Abstract We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorect...

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Autores principales:	Vivian W. Xue, Moon T. Cheung, Pak T. Chan, Lewis L. Y. Luk, Vivian H. Lee, Thomas C. Au, Allen C. Yu, William C. S. Cho, Hin Fung Andy Tsang, Amanda K. Chan, S. C. Cesar Wong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/920747b01adf430ab2b4c76f1356e300
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spelling	oai:doaj.org-article:920747b01adf430ab2b4c76f1356e3002021-12-02T16:08:16ZNon-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing10.1038/s41598-019-49445-x2045-2322https://doaj.org/article/920747b01adf430ab2b4c76f1356e3002019-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49445-xhttps://doaj.org/toc/2045-2322Abstract We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed that GSK3A and RHOA were differential expressed genes identified by a cut-off of fold change >2 and adjusted P value < 0.05. More detailed analysis showed that the expression of both GSK3A (0.01-fold with adjusted P < 1 × 10−6) and RHOA (0.35-fold with adjusted P < 0.01) in adenoma patients was significantly lower than those in normal healthy subjects. Based on the enrichment analysis of biological process for potential markers, we found that the regulation of programmed cell death (GO: 0043067; GO: 0043069), regulation of cell death (GO: 0010941; GO: 0060548) and cell differentiation (GO: 0021861) were the main processes involved in adenoma formation. In summary, this study is a cutting-edge research on the detection of plasma mRNA in colorectal adenoma patients and normal healthy subjects.Vivian W. XueMoon T. CheungPak T. ChanLewis L. Y. LukVivian H. LeeThomas C. AuAllen C. YuWilliam C. S. ChoHin Fung Andy TsangAmanda K. ChanS. C. Cesar WongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vivian W. Xue Moon T. Cheung Pak T. Chan Lewis L. Y. Luk Vivian H. Lee Thomas C. Au Allen C. Yu William C. S. Cho Hin Fung Andy Tsang Amanda K. Chan S. C. Cesar Wong Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing
description	Abstract We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed that GSK3A and RHOA were differential expressed genes identified by a cut-off of fold change >2 and adjusted P value < 0.05. More detailed analysis showed that the expression of both GSK3A (0.01-fold with adjusted P < 1 × 10−6) and RHOA (0.35-fold with adjusted P < 0.01) in adenoma patients was significantly lower than those in normal healthy subjects. Based on the enrichment analysis of biological process for potential markers, we found that the regulation of programmed cell death (GO: 0043067; GO: 0043069), regulation of cell death (GO: 0010941; GO: 0060548) and cell differentiation (GO: 0021861) were the main processes involved in adenoma formation. In summary, this study is a cutting-edge research on the detection of plasma mRNA in colorectal adenoma patients and normal healthy subjects.
format	article
author	Vivian W. Xue Moon T. Cheung Pak T. Chan Lewis L. Y. Luk Vivian H. Lee Thomas C. Au Allen C. Yu William C. S. Cho Hin Fung Andy Tsang Amanda K. Chan S. C. Cesar Wong
author_facet	Vivian W. Xue Moon T. Cheung Pak T. Chan Lewis L. Y. Luk Vivian H. Lee Thomas C. Au Allen C. Yu William C. S. Cho Hin Fung Andy Tsang Amanda K. Chan S. C. Cesar Wong
author_sort	Vivian W. Xue
title	Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing
title_short	Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing
title_full	Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing
title_fullStr	Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing
title_full_unstemmed	Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing
title_sort	non-invasive potential circulating mrna markers for colorectal adenoma using targeted sequencing
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/920747b01adf430ab2b4c76f1356e300
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Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing

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