Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease

Abstract There is increasing evidence that some functionally related, co-expressed genes cluster within eukaryotic genomes. We present a novel pipeline that delineates such eukaryotic gene clusters. Using this tool for bread wheat, we uncovered 44 clusters of genes that are responsive to the fungal...

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Autores principales:	Alexandre Perochon, Harriet R. Benbow, Katarzyna Ślęczka-Brady, Keshav B. Malla, Fiona M. Doohan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/920bee864ca84e5a88c4c3fd3057993b
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spelling	oai:doaj.org-article:920bee864ca84e5a88c4c3fd3057993b2021-12-02T14:23:05ZAnalysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease10.1038/s41598-021-86362-42045-2322https://doaj.org/article/920bee864ca84e5a88c4c3fd3057993b2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86362-4https://doaj.org/toc/2045-2322Abstract There is increasing evidence that some functionally related, co-expressed genes cluster within eukaryotic genomes. We present a novel pipeline that delineates such eukaryotic gene clusters. Using this tool for bread wheat, we uncovered 44 clusters of genes that are responsive to the fungal pathogen Fusarium graminearum. As expected, these Fusarium-responsive gene clusters (FRGCs) included metabolic gene clusters, many of which are associated with disease resistance, but hitherto not described for wheat. However, the majority of the FRGCs are non-metabolic, many of which contain clusters of paralogues, including those implicated in plant disease responses, such as glutathione transferases, MAP kinases, and germin-like proteins. 20 of the FRGCs encode nonhomologous, non-metabolic genes (including defence-related genes). One of these clusters includes the characterised Fusarium resistance orphan gene, TaFROG. Eight of the FRGCs map within 6 FHB resistance loci. One small QTL on chromosome 7D (4.7 Mb) encodes eight Fusarium-responsive genes, five of which are within a FRGC. This study provides a new tool to identify genomic regions enriched in genes responsive to specific traits of interest and applied herein it highlighted gene families, genetic loci and biological pathways of importance in the response of wheat to disease.Alexandre PerochonHarriet R. BenbowKatarzyna Ślęczka-BradyKeshav B. MallaFiona M. DoohanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandre Perochon Harriet R. Benbow Katarzyna Ślęczka-Brady Keshav B. Malla Fiona M. Doohan Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease
description	Abstract There is increasing evidence that some functionally related, co-expressed genes cluster within eukaryotic genomes. We present a novel pipeline that delineates such eukaryotic gene clusters. Using this tool for bread wheat, we uncovered 44 clusters of genes that are responsive to the fungal pathogen Fusarium graminearum. As expected, these Fusarium-responsive gene clusters (FRGCs) included metabolic gene clusters, many of which are associated with disease resistance, but hitherto not described for wheat. However, the majority of the FRGCs are non-metabolic, many of which contain clusters of paralogues, including those implicated in plant disease responses, such as glutathione transferases, MAP kinases, and germin-like proteins. 20 of the FRGCs encode nonhomologous, non-metabolic genes (including defence-related genes). One of these clusters includes the characterised Fusarium resistance orphan gene, TaFROG. Eight of the FRGCs map within 6 FHB resistance loci. One small QTL on chromosome 7D (4.7 Mb) encodes eight Fusarium-responsive genes, five of which are within a FRGC. This study provides a new tool to identify genomic regions enriched in genes responsive to specific traits of interest and applied herein it highlighted gene families, genetic loci and biological pathways of importance in the response of wheat to disease.
format	article
author	Alexandre Perochon Harriet R. Benbow Katarzyna Ślęczka-Brady Keshav B. Malla Fiona M. Doohan
author_facet	Alexandre Perochon Harriet R. Benbow Katarzyna Ślęczka-Brady Keshav B. Malla Fiona M. Doohan
author_sort	Alexandre Perochon
title	Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease
title_short	Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease
title_full	Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease
title_fullStr	Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease
title_full_unstemmed	Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease
title_sort	analysis of the chromosomal clustering of fusarium-responsive wheat genes uncovers new players in the defence against head blight disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/920bee864ca84e5a88c4c3fd3057993b
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Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease

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