Involvement of GATA1 and Sp3 in the activation of the murine STING gene promoter in NIH3T3 cells

Abstract Stimulator of Interferon Gene (STING) is a key mediator of innate immune signaling. STING plays a pivotal role in the pathogenesis of many diseases including infectious diseases, auto-immune diseases and cancer. Many studies have been carried out recently in the field of STING-regulated pat...

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Autores principales:	Yan-Yan Xu, Rui Jin, Guo-Ping Zhou, Hua-Guo Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9217eb2fd9ec45ef8ee24e0b91145b09
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spelling	oai:doaj.org-article:9217eb2fd9ec45ef8ee24e0b91145b092021-12-02T11:52:31ZInvolvement of GATA1 and Sp3 in the activation of the murine STING gene promoter in NIH3T3 cells10.1038/s41598-017-02242-w2045-2322https://doaj.org/article/9217eb2fd9ec45ef8ee24e0b91145b092017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02242-whttps://doaj.org/toc/2045-2322Abstract Stimulator of Interferon Gene (STING) is a key mediator of innate immune signaling. STING plays a pivotal role in the pathogenesis of many diseases including infectious diseases, auto-immune diseases and cancer. Many studies have been carried out recently in the field of STING-regulated pathway, however, rarely of transcriptional mechanisms. To characterize the murine STING (mSTING) promoter, we cloned a series of different nucleotide sequences of the 5′-flanking region of the mSTING gene. Transient transfection of promoter-reporter recombinant plasmids and luciferase assay illustrated the region (−77/+177) relative to the transcription start site (TSS) of the mSTING gene was sufficient for full promoter activity. This region contains GATA1, IK2, Sp1/Sp3 and STAT putative transcription factor binding sites. Mutation of GATA1 or Sp1/Sp3 sites led to obvious decrease of the mSTING promoter activity. Overexpression of GATA1 and Sp3 enhanced the mSTING promoter activity, whereas knockdown of GATA1 and Sp3 by a siRNA strategy significantly reduced the transcription activity. Chromatin immunoprecipitation assays demonstrated that GATA1 and Sp3 interact with the mSTING promoter in vivo. These results provided the first analysis of mSTING promoter and demonstrated that transcription factor GATA1 and Sp3 positively regulate the basal transcription of the mSTING gene.Yan-Yan XuRui JinGuo-Ping ZhouHua-Guo XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan-Yan Xu Rui Jin Guo-Ping Zhou Hua-Guo Xu Involvement of GATA1 and Sp3 in the activation of the murine STING gene promoter in NIH3T3 cells
description	Abstract Stimulator of Interferon Gene (STING) is a key mediator of innate immune signaling. STING plays a pivotal role in the pathogenesis of many diseases including infectious diseases, auto-immune diseases and cancer. Many studies have been carried out recently in the field of STING-regulated pathway, however, rarely of transcriptional mechanisms. To characterize the murine STING (mSTING) promoter, we cloned a series of different nucleotide sequences of the 5′-flanking region of the mSTING gene. Transient transfection of promoter-reporter recombinant plasmids and luciferase assay illustrated the region (−77/+177) relative to the transcription start site (TSS) of the mSTING gene was sufficient for full promoter activity. This region contains GATA1, IK2, Sp1/Sp3 and STAT putative transcription factor binding sites. Mutation of GATA1 or Sp1/Sp3 sites led to obvious decrease of the mSTING promoter activity. Overexpression of GATA1 and Sp3 enhanced the mSTING promoter activity, whereas knockdown of GATA1 and Sp3 by a siRNA strategy significantly reduced the transcription activity. Chromatin immunoprecipitation assays demonstrated that GATA1 and Sp3 interact with the mSTING promoter in vivo. These results provided the first analysis of mSTING promoter and demonstrated that transcription factor GATA1 and Sp3 positively regulate the basal transcription of the mSTING gene.
format	article
author	Yan-Yan Xu Rui Jin Guo-Ping Zhou Hua-Guo Xu
author_facet	Yan-Yan Xu Rui Jin Guo-Ping Zhou Hua-Guo Xu
author_sort	Yan-Yan Xu
title	Involvement of GATA1 and Sp3 in the activation of the murine STING gene promoter in NIH3T3 cells
title_short	Involvement of GATA1 and Sp3 in the activation of the murine STING gene promoter in NIH3T3 cells
title_full	Involvement of GATA1 and Sp3 in the activation of the murine STING gene promoter in NIH3T3 cells
title_fullStr	Involvement of GATA1 and Sp3 in the activation of the murine STING gene promoter in NIH3T3 cells
title_full_unstemmed	Involvement of GATA1 and Sp3 in the activation of the murine STING gene promoter in NIH3T3 cells
title_sort	involvement of gata1 and sp3 in the activation of the murine sting gene promoter in nih3t3 cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9217eb2fd9ec45ef8ee24e0b91145b09
work_keys_str_mv	AT yanyanxu involvementofgata1andsp3intheactivationofthemurinestinggenepromoterinnih3t3cells AT ruijin involvementofgata1andsp3intheactivationofthemurinestinggenepromoterinnih3t3cells AT guopingzhou involvementofgata1andsp3intheactivationofthemurinestinggenepromoterinnih3t3cells AT huaguoxu involvementofgata1andsp3intheactivationofthemurinestinggenepromoterinnih3t3cells
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Involvement of GATA1 and Sp3 in the activation of the murine STING gene promoter in NIH3T3 cells

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